Estimating rumen degradability of forages from semi-natural grasslands, using nylon bag and gas production techniques

M.H. Bruinenberg, A.H. van Gelder, P. Gonzalez Perez, V.A. Hindle, J.W. Cone

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5 Citations (Scopus)


To determine the ruminal digestion of forages from extensively managed semi-natural grasslands, degradation characteristics and kinetics of silages of three different forages in the rumen of lactating dairy cows were estimated in vitro using the gas production technique (GPT), and in situ using the nylon bag technique. Silages originated from intensively managed grassland (IMG), extensively managed species-poor grassland (SPP) and extensively managed species-rich grassland (SPR). Some individual species originating from extensively managed species-poor and species-rich grasslands were used to estimate their degradability with GPT in order to compare the differences among the species occurring on these two types of grassland. All samples were also analysed for in vitro organic matter digestibility. In situ degradability was estimated by nylon bag incubation in the rumen of three dairy cows at two different periods. Rate of organic matter degradation was highest for IMG (4.93 and 4.54% h-1), intermediate for SPR (3.50 and 4.11% h-1) and lowest for SPP (2.62 and 2.72% h-1). The rates of degradation for protein and neutral detergent fibre were highest for IMG. The undegradable fraction was the same for SPP and SPR. Highest cell wall fermentation was observed for IMG and lowest for SPP, but SPP and SPR did not differ statistically in this respect. Cell wall degradability of the individual species from the species-poor and species-rich grasslands were highest for Lolium perenne and Dactylis glomerata and lowest for Lathyrus pratensis and Anthriscus sylvestris. It is concluded that SPR has the highest potential to become a component of the dairy cow ration.
Original languageEnglish
Pages (from-to)351-368
JournalNetherlands Journal of Agricultural Science
Issue number4
Publication statusPublished - 2004


  • rumen digestion
  • forage
  • grasses
  • grasslands
  • silage
  • dairy-cows
  • degradation
  • digestibility
  • fermentation
  • digestion
  • ryegrass
  • nitrogen
  • maturity
  • kinetics
  • protein

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