Establishment of a cell line from Chrysodeixis chalcites permissive for Chrysodeixis chalcites and Trichoplusia ni nucleopolyhedrovirus

F. Xu, D.E. Lynn, E.C. Roode, D. Muñoz, J.W.M. van Lent, J.M. Vlak, M.M. van Oers

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

A new cell line was established from the embryos of the insect Chrysodeixis chalcites (Lepidoptera, Noctuidae, Plusiinae). The cell line contains several morphologically different cell types and was distinguished from three other lepidopteran cell lines propagated in the laboratory by DNA amplification fingerprinting. The cultured cells, which we officially named WU-CcE-1 cells, were permissive for infection by C. chalcites nucleopolyhedrovirus (ChchNPV) and large numbers of occlusion bodies were produced that retained their infectivity for C. chalcites larvae. The CcE-1 cells were also permissive for Trichoplusia ni single nucleopolyhedrovirus (TnSNPV). ChchNPV could be passaged in these cells for at least four passages indicating that budded virus production was supported. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Helicoverpa armigera (Hear) NPV both induced apoptosis in these cells. The results obtained indicate that the CcE-1 cell line will be a useful tool in the study of both ChchNPV and TnSNPV
Original languageEnglish
Pages (from-to)56-62
JournalJournal of Invertebrate Pathology
Volume105
Issue number1
DOIs
Publication statusPublished - 2010

Fingerprint

Chrysodeixis chalcites
Trichoplusia ni
infectivity
apoptosis
Baculoviridae
embryo
amplification
virus
cell lines
Trichoplusia ni single nucleopolyhedrovirus
insect
larva
DNA
cells
Lepidoptera
Autographa californica multiple nucleopolyhedrovirus
Helicoverpa armigera
Noctuidae
cultured cells
pathogenicity

Keywords

  • nuclear polyhedrosis-virus
  • single-nucleocapsid nucleopolyhedrovirus
  • dna photolyase genes
  • insect cells
  • baculovirus vectors
  • identification
  • infectivity
  • protein
  • genome
  • replication

Cite this

@article{4baf2af4309749ceb411173b1b9a9450,
title = "Establishment of a cell line from Chrysodeixis chalcites permissive for Chrysodeixis chalcites and Trichoplusia ni nucleopolyhedrovirus",
abstract = "A new cell line was established from the embryos of the insect Chrysodeixis chalcites (Lepidoptera, Noctuidae, Plusiinae). The cell line contains several morphologically different cell types and was distinguished from three other lepidopteran cell lines propagated in the laboratory by DNA amplification fingerprinting. The cultured cells, which we officially named WU-CcE-1 cells, were permissive for infection by C. chalcites nucleopolyhedrovirus (ChchNPV) and large numbers of occlusion bodies were produced that retained their infectivity for C. chalcites larvae. The CcE-1 cells were also permissive for Trichoplusia ni single nucleopolyhedrovirus (TnSNPV). ChchNPV could be passaged in these cells for at least four passages indicating that budded virus production was supported. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Helicoverpa armigera (Hear) NPV both induced apoptosis in these cells. The results obtained indicate that the CcE-1 cell line will be a useful tool in the study of both ChchNPV and TnSNPV",
keywords = "nuclear polyhedrosis-virus, single-nucleocapsid nucleopolyhedrovirus, dna photolyase genes, insect cells, baculovirus vectors, identification, infectivity, protein, genome, replication",
author = "F. Xu and D.E. Lynn and E.C. Roode and D. Mu{\~n}oz and {van Lent}, J.W.M. and J.M. Vlak and {van Oers}, M.M.",
year = "2010",
doi = "10.1016/j.jip.2010.05.008",
language = "English",
volume = "105",
pages = "56--62",
journal = "Journal of Invertebrate Pathology",
issn = "0022-2011",
publisher = "Elsevier",
number = "1",

}

Establishment of a cell line from Chrysodeixis chalcites permissive for Chrysodeixis chalcites and Trichoplusia ni nucleopolyhedrovirus. / Xu, F.; Lynn, D.E.; Roode, E.C.; Muñoz, D.; van Lent, J.W.M.; Vlak, J.M.; van Oers, M.M.

In: Journal of Invertebrate Pathology, Vol. 105, No. 1, 2010, p. 56-62.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Establishment of a cell line from Chrysodeixis chalcites permissive for Chrysodeixis chalcites and Trichoplusia ni nucleopolyhedrovirus

AU - Xu, F.

AU - Lynn, D.E.

AU - Roode, E.C.

AU - Muñoz, D.

AU - van Lent, J.W.M.

AU - Vlak, J.M.

AU - van Oers, M.M.

PY - 2010

Y1 - 2010

N2 - A new cell line was established from the embryos of the insect Chrysodeixis chalcites (Lepidoptera, Noctuidae, Plusiinae). The cell line contains several morphologically different cell types and was distinguished from three other lepidopteran cell lines propagated in the laboratory by DNA amplification fingerprinting. The cultured cells, which we officially named WU-CcE-1 cells, were permissive for infection by C. chalcites nucleopolyhedrovirus (ChchNPV) and large numbers of occlusion bodies were produced that retained their infectivity for C. chalcites larvae. The CcE-1 cells were also permissive for Trichoplusia ni single nucleopolyhedrovirus (TnSNPV). ChchNPV could be passaged in these cells for at least four passages indicating that budded virus production was supported. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Helicoverpa armigera (Hear) NPV both induced apoptosis in these cells. The results obtained indicate that the CcE-1 cell line will be a useful tool in the study of both ChchNPV and TnSNPV

AB - A new cell line was established from the embryos of the insect Chrysodeixis chalcites (Lepidoptera, Noctuidae, Plusiinae). The cell line contains several morphologically different cell types and was distinguished from three other lepidopteran cell lines propagated in the laboratory by DNA amplification fingerprinting. The cultured cells, which we officially named WU-CcE-1 cells, were permissive for infection by C. chalcites nucleopolyhedrovirus (ChchNPV) and large numbers of occlusion bodies were produced that retained their infectivity for C. chalcites larvae. The CcE-1 cells were also permissive for Trichoplusia ni single nucleopolyhedrovirus (TnSNPV). ChchNPV could be passaged in these cells for at least four passages indicating that budded virus production was supported. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Helicoverpa armigera (Hear) NPV both induced apoptosis in these cells. The results obtained indicate that the CcE-1 cell line will be a useful tool in the study of both ChchNPV and TnSNPV

KW - nuclear polyhedrosis-virus

KW - single-nucleocapsid nucleopolyhedrovirus

KW - dna photolyase genes

KW - insect cells

KW - baculovirus vectors

KW - identification

KW - infectivity

KW - protein

KW - genome

KW - replication

U2 - 10.1016/j.jip.2010.05.008

DO - 10.1016/j.jip.2010.05.008

M3 - Article

VL - 105

SP - 56

EP - 62

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

SN - 0022-2011

IS - 1

ER -