Establishing in vitro Zinnia elegans cell suspension culture with high tracheary elements differentiation

P. Twumasi, J.H.N. Schel, W. van Ieperen, E.J. Woltering, A.M.C. Emons

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

The Zinnia elegans mesophyll cell culture is a useful system for xylogenesis studies. The system is associated with highly synchronous tracheary element (TE) differentiation, making it more suitable for molecular studies requiring larger amounts of molecular isolates, such as mRNA and proteins and for studying cellulose synthesis. There is, however, the problem of non-uniformity and significant variations in the yields of TEs (%TE). One possible cause for this variability in the %TE could be the lack of a standardized experimental protocol in various research laboratories for establishing the Zinnia culture. Mesophyll cells isolated from the first true leaves of Z. elegans var Envy seedlings of approximately 14 days old were cultured in vitro and differentiated into TEs. The xylogenic culture medium was supplied with 1 mg/l each of benzylaminopurine (BA) and ¿-naphthalene acetic acid (NAA). Application of this improved culture method resulted in stable and reproducible amounts of TE as high as 76% in the Zinnia culture. The increase was mainly due to conditioning of the mesophyll cell culture and adjustments of the phytohormonal balance in the cultures. Also, certain biochemical and cytological methods have been shown to reliably monitor progress of TE differentiation. We conclude that, with the adoption of current improvement in the xylogenic Z. elegans culture, higher amounts of tracheary elements can be produced. This successful outcome raises the potential of the Zinnia system as a suitable model for cellulose and xylogenesis research.
LanguageEnglish
Pages524-533
JournalCell Biology International
Volume33
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

Mesophyll Cells
Suspensions
Cell Culture Techniques
Cellulose
Seedlings
Research
Acetic Acid
Culture Media
Messenger RNA
In Vitro Techniques
Proteins

Keywords

  • cellulose synthesis
  • death
  • mesophyll
  • lignification
  • xylogenesis
  • involvement
  • apoptosis
  • xylem

Cite this

@article{6f125b8ad5c84f59b6de78e7d27aed7b,
title = "Establishing in vitro Zinnia elegans cell suspension culture with high tracheary elements differentiation",
abstract = "The Zinnia elegans mesophyll cell culture is a useful system for xylogenesis studies. The system is associated with highly synchronous tracheary element (TE) differentiation, making it more suitable for molecular studies requiring larger amounts of molecular isolates, such as mRNA and proteins and for studying cellulose synthesis. There is, however, the problem of non-uniformity and significant variations in the yields of TEs ({\%}TE). One possible cause for this variability in the {\%}TE could be the lack of a standardized experimental protocol in various research laboratories for establishing the Zinnia culture. Mesophyll cells isolated from the first true leaves of Z. elegans var Envy seedlings of approximately 14 days old were cultured in vitro and differentiated into TEs. The xylogenic culture medium was supplied with 1 mg/l each of benzylaminopurine (BA) and ¿-naphthalene acetic acid (NAA). Application of this improved culture method resulted in stable and reproducible amounts of TE as high as 76{\%} in the Zinnia culture. The increase was mainly due to conditioning of the mesophyll cell culture and adjustments of the phytohormonal balance in the cultures. Also, certain biochemical and cytological methods have been shown to reliably monitor progress of TE differentiation. We conclude that, with the adoption of current improvement in the xylogenic Z. elegans culture, higher amounts of tracheary elements can be produced. This successful outcome raises the potential of the Zinnia system as a suitable model for cellulose and xylogenesis research.",
keywords = "cellulose synthesis, death, mesophyll, lignification, xylogenesis, involvement, apoptosis, xylem",
author = "P. Twumasi and J.H.N. Schel and {van Ieperen}, W. and E.J. Woltering and A.M.C. Emons",
note = "009/09",
year = "2009",
doi = "10.1016/j.cellbi.2009.01.019",
language = "English",
volume = "33",
pages = "524--533",
journal = "Cell Biology International",
issn = "1065-6995",
publisher = "Wiley",
number = "4",

}

Establishing in vitro Zinnia elegans cell suspension culture with high tracheary elements differentiation. / Twumasi, P.; Schel, J.H.N.; van Ieperen, W.; Woltering, E.J.; Emons, A.M.C.

In: Cell Biology International, Vol. 33, No. 4, 2009, p. 524-533.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Establishing in vitro Zinnia elegans cell suspension culture with high tracheary elements differentiation

AU - Twumasi, P.

AU - Schel, J.H.N.

AU - van Ieperen, W.

AU - Woltering, E.J.

AU - Emons, A.M.C.

N1 - 009/09

PY - 2009

Y1 - 2009

N2 - The Zinnia elegans mesophyll cell culture is a useful system for xylogenesis studies. The system is associated with highly synchronous tracheary element (TE) differentiation, making it more suitable for molecular studies requiring larger amounts of molecular isolates, such as mRNA and proteins and for studying cellulose synthesis. There is, however, the problem of non-uniformity and significant variations in the yields of TEs (%TE). One possible cause for this variability in the %TE could be the lack of a standardized experimental protocol in various research laboratories for establishing the Zinnia culture. Mesophyll cells isolated from the first true leaves of Z. elegans var Envy seedlings of approximately 14 days old were cultured in vitro and differentiated into TEs. The xylogenic culture medium was supplied with 1 mg/l each of benzylaminopurine (BA) and ¿-naphthalene acetic acid (NAA). Application of this improved culture method resulted in stable and reproducible amounts of TE as high as 76% in the Zinnia culture. The increase was mainly due to conditioning of the mesophyll cell culture and adjustments of the phytohormonal balance in the cultures. Also, certain biochemical and cytological methods have been shown to reliably monitor progress of TE differentiation. We conclude that, with the adoption of current improvement in the xylogenic Z. elegans culture, higher amounts of tracheary elements can be produced. This successful outcome raises the potential of the Zinnia system as a suitable model for cellulose and xylogenesis research.

AB - The Zinnia elegans mesophyll cell culture is a useful system for xylogenesis studies. The system is associated with highly synchronous tracheary element (TE) differentiation, making it more suitable for molecular studies requiring larger amounts of molecular isolates, such as mRNA and proteins and for studying cellulose synthesis. There is, however, the problem of non-uniformity and significant variations in the yields of TEs (%TE). One possible cause for this variability in the %TE could be the lack of a standardized experimental protocol in various research laboratories for establishing the Zinnia culture. Mesophyll cells isolated from the first true leaves of Z. elegans var Envy seedlings of approximately 14 days old were cultured in vitro and differentiated into TEs. The xylogenic culture medium was supplied with 1 mg/l each of benzylaminopurine (BA) and ¿-naphthalene acetic acid (NAA). Application of this improved culture method resulted in stable and reproducible amounts of TE as high as 76% in the Zinnia culture. The increase was mainly due to conditioning of the mesophyll cell culture and adjustments of the phytohormonal balance in the cultures. Also, certain biochemical and cytological methods have been shown to reliably monitor progress of TE differentiation. We conclude that, with the adoption of current improvement in the xylogenic Z. elegans culture, higher amounts of tracheary elements can be produced. This successful outcome raises the potential of the Zinnia system as a suitable model for cellulose and xylogenesis research.

KW - cellulose synthesis

KW - death

KW - mesophyll

KW - lignification

KW - xylogenesis

KW - involvement

KW - apoptosis

KW - xylem

U2 - 10.1016/j.cellbi.2009.01.019

DO - 10.1016/j.cellbi.2009.01.019

M3 - Article

VL - 33

SP - 524

EP - 533

JO - Cell Biology International

T2 - Cell Biology International

JF - Cell Biology International

SN - 1065-6995

IS - 4

ER -