Essentials to assess the impact of bio-based practices on soil life - sampling strategies at landscape scale

C.W. Quist, G. Gort, D.J. Brus, C. Mulder, A. Termorshuizen, J. Bakker, J. Helder

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

To assess and monitor the impact of novel bio-based economy related crops and processes on soil life, ideally organismal groups with representatives in all major multiple trophic layers should be used as reporters. Nematodes are represented in each of the three major thropic levels in the soil food web, are highly abundant, and easily extractable from soil (Neher et al., 2005). Moreover, the availability of quantitative molecular assays for a wide range of nematode taxa (Vervoort et al., 2012), allows for a high throughput analysis of samples. Detailed insights in spatial distributions of nematode taxa at meso- (~1 m2) and macro- (~hectare) scale are a prerequisite for (a) the selection of the most informative taxa, and (b) the design of strategies for the affordable and accurate sampling of relatively large areas. To address these two issues ~ 1,500 spatially distant soil samples were collected and ~ 40,000 qPCR reactions were run to detect and quantify ~ 25 nematode taxa per soil sample. This was done for the three main soil types in The Netherlands, namely marine clay, river clay and sandy soil. Results demonstrate that at meso-scale a relatively extensive sampling strategy is sufficient to get insight in the densities of non-rare taxa, and the same strategy can be used for all major Dutch soil types irrespective whether this involves arable or natural soil ecosystems. Hence, statistically robust sampling of nematode communities at landscape scale is far less labor intensive than previously expected. As a next step we present a geo-statistical sampling approach to investigate spatial distribution of nematode taxa on macro-scale.
LanguageEnglish
Title of host publicationProceedings of the BE-Basic Symposium 2015
Pages134-134
Publication statusPublished - 2015
EventBE-Basic Symposium 2015, Noordwijkerhout, The Netherlands -
Duration: 31 Mar 20151 Apr 2015

Conference

ConferenceBE-Basic Symposium 2015, Noordwijkerhout, The Netherlands
Period31/03/151/04/15

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nematode
sampling
soil
soil type
spatial distribution
soil ecosystem
clay soil
sandy soil
food web
labor
assay
clay
crop
river

Cite this

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title = "Essentials to assess the impact of bio-based practices on soil life - sampling strategies at landscape scale",
abstract = "To assess and monitor the impact of novel bio-based economy related crops and processes on soil life, ideally organismal groups with representatives in all major multiple trophic layers should be used as reporters. Nematodes are represented in each of the three major thropic levels in the soil food web, are highly abundant, and easily extractable from soil (Neher et al., 2005). Moreover, the availability of quantitative molecular assays for a wide range of nematode taxa (Vervoort et al., 2012), allows for a high throughput analysis of samples. Detailed insights in spatial distributions of nematode taxa at meso- (~1 m2) and macro- (~hectare) scale are a prerequisite for (a) the selection of the most informative taxa, and (b) the design of strategies for the affordable and accurate sampling of relatively large areas. To address these two issues ~ 1,500 spatially distant soil samples were collected and ~ 40,000 qPCR reactions were run to detect and quantify ~ 25 nematode taxa per soil sample. This was done for the three main soil types in The Netherlands, namely marine clay, river clay and sandy soil. Results demonstrate that at meso-scale a relatively extensive sampling strategy is sufficient to get insight in the densities of non-rare taxa, and the same strategy can be used for all major Dutch soil types irrespective whether this involves arable or natural soil ecosystems. Hence, statistically robust sampling of nematode communities at landscape scale is far less labor intensive than previously expected. As a next step we present a geo-statistical sampling approach to investigate spatial distribution of nematode taxa on macro-scale.",
author = "C.W. Quist and G. Gort and D.J. Brus and C. Mulder and A. Termorshuizen and J. Bakker and J. Helder",
year = "2015",
language = "English",
pages = "134--134",
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}

Quist, CW, Gort, G, Brus, DJ, Mulder, C, Termorshuizen, A, Bakker, J & Helder, J 2015, Essentials to assess the impact of bio-based practices on soil life - sampling strategies at landscape scale. in Proceedings of the BE-Basic Symposium 2015. pp. 134-134, BE-Basic Symposium 2015, Noordwijkerhout, The Netherlands, 31/03/15.

Essentials to assess the impact of bio-based practices on soil life - sampling strategies at landscape scale. / Quist, C.W.; Gort, G.; Brus, D.J.; Mulder, C.; Termorshuizen, A.; Bakker, J.; Helder, J.

Proceedings of the BE-Basic Symposium 2015. 2015. p. 134-134.

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

TY - CHAP

T1 - Essentials to assess the impact of bio-based practices on soil life - sampling strategies at landscape scale

AU - Quist, C.W.

AU - Gort, G.

AU - Brus, D.J.

AU - Mulder, C.

AU - Termorshuizen, A.

AU - Bakker, J.

AU - Helder, J.

PY - 2015

Y1 - 2015

N2 - To assess and monitor the impact of novel bio-based economy related crops and processes on soil life, ideally organismal groups with representatives in all major multiple trophic layers should be used as reporters. Nematodes are represented in each of the three major thropic levels in the soil food web, are highly abundant, and easily extractable from soil (Neher et al., 2005). Moreover, the availability of quantitative molecular assays for a wide range of nematode taxa (Vervoort et al., 2012), allows for a high throughput analysis of samples. Detailed insights in spatial distributions of nematode taxa at meso- (~1 m2) and macro- (~hectare) scale are a prerequisite for (a) the selection of the most informative taxa, and (b) the design of strategies for the affordable and accurate sampling of relatively large areas. To address these two issues ~ 1,500 spatially distant soil samples were collected and ~ 40,000 qPCR reactions were run to detect and quantify ~ 25 nematode taxa per soil sample. This was done for the three main soil types in The Netherlands, namely marine clay, river clay and sandy soil. Results demonstrate that at meso-scale a relatively extensive sampling strategy is sufficient to get insight in the densities of non-rare taxa, and the same strategy can be used for all major Dutch soil types irrespective whether this involves arable or natural soil ecosystems. Hence, statistically robust sampling of nematode communities at landscape scale is far less labor intensive than previously expected. As a next step we present a geo-statistical sampling approach to investigate spatial distribution of nematode taxa on macro-scale.

AB - To assess and monitor the impact of novel bio-based economy related crops and processes on soil life, ideally organismal groups with representatives in all major multiple trophic layers should be used as reporters. Nematodes are represented in each of the three major thropic levels in the soil food web, are highly abundant, and easily extractable from soil (Neher et al., 2005). Moreover, the availability of quantitative molecular assays for a wide range of nematode taxa (Vervoort et al., 2012), allows for a high throughput analysis of samples. Detailed insights in spatial distributions of nematode taxa at meso- (~1 m2) and macro- (~hectare) scale are a prerequisite for (a) the selection of the most informative taxa, and (b) the design of strategies for the affordable and accurate sampling of relatively large areas. To address these two issues ~ 1,500 spatially distant soil samples were collected and ~ 40,000 qPCR reactions were run to detect and quantify ~ 25 nematode taxa per soil sample. This was done for the three main soil types in The Netherlands, namely marine clay, river clay and sandy soil. Results demonstrate that at meso-scale a relatively extensive sampling strategy is sufficient to get insight in the densities of non-rare taxa, and the same strategy can be used for all major Dutch soil types irrespective whether this involves arable or natural soil ecosystems. Hence, statistically robust sampling of nematode communities at landscape scale is far less labor intensive than previously expected. As a next step we present a geo-statistical sampling approach to investigate spatial distribution of nematode taxa on macro-scale.

M3 - Abstract

SP - 134

EP - 134

BT - Proceedings of the BE-Basic Symposium 2015

ER -

Quist CW, Gort G, Brus DJ, Mulder C, Termorshuizen A, Bakker J et al. Essentials to assess the impact of bio-based practices on soil life - sampling strategies at landscape scale. In Proceedings of the BE-Basic Symposium 2015. 2015. p. 134-134