Erythrocyte fouling on micro-engineered membranes

Levy I. Amar*, Daniela Guisado, Monica Faria, James P. Jones, Cees J.M. van Rijn, Michael I. Hill, Edward F. Leonard

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Crossflow microfiltration of plasma from blood through microsieves in a microchannel is potentially useful in many biomedical applications, including clinically as a wearable water removal device under development by the authors. We report experiments that correlate filtration rates, transmembrane pressures (TMP) and shear rates during filtration through a microscopically high channel bounded by a low intrinsic resistance photolithographically-produced porous semiconductor membrane. These experiments allowed observation of erythrocyte behavior at the filtering surface and showed how their unique deformability properties dominated filtration resistance. At low filtration rates (corresponding to low TMP), they rolled along the filter surface, but at higher filtration rates (corresponding to higher TMP), they anchored themselves to the filter membrane, forming a self-assembled, incomplete monolayer. The incompleteness of the layer was an essential feature of the monolayer’s ability to support sustainable filtration. Maximum steady-state filtration flux was a function of wall shear rate, as predicted by conventional crossflow filtration theory, but, contrary to theories based on convective diffusion, showed weak dependence of filtration on erythrocyte concentration. Post-filtration scanning electron micrographs revealed significant capture and deformation of erythrocytes in all filter pores in the range 0.25 to 2 μm diameter. We report filtration rates through these filters and describe a largely unrecognized mechanism that allows stable filtration in the presence of substantial cell layers.

Original languageEnglish
Article number55
JournalBiomedical Microdevices
Volume20
Issue number3
DOIs
Publication statusPublished - 1 Sep 2018

Fingerprint

Fouling
Erythrocytes
Membranes
Pressure
Shear deformation
Device Removal
Semiconductors
Aptitude
Microfiltration
Self assembled monolayers
Formability
Microchannels
Monolayers
Blood
Experiments
Cells
Electrons
Semiconductor materials
Fluxes
Scanning

Keywords

  • Blood
  • Cross-flow
  • Erythrocytes
  • Fouling
  • Microfiltration model
  • Microfluidics
  • Microsieve
  • Nanopores
  • Photolithography
  • Sieve

Cite this

Amar, L. I., Guisado, D., Faria, M., Jones, J. P., van Rijn, C. J. M., Hill, M. I., & Leonard, E. F. (2018). Erythrocyte fouling on micro-engineered membranes. Biomedical Microdevices, 20(3), [55]. https://doi.org/10.1007/s10544-018-0297-1
Amar, Levy I. ; Guisado, Daniela ; Faria, Monica ; Jones, James P. ; van Rijn, Cees J.M. ; Hill, Michael I. ; Leonard, Edward F. / Erythrocyte fouling on micro-engineered membranes. In: Biomedical Microdevices. 2018 ; Vol. 20, No. 3.
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author = "Amar, {Levy I.} and Daniela Guisado and Monica Faria and Jones, {James P.} and {van Rijn}, {Cees J.M.} and Hill, {Michael I.} and Leonard, {Edward F.}",
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Amar, LI, Guisado, D, Faria, M, Jones, JP, van Rijn, CJM, Hill, MI & Leonard, EF 2018, 'Erythrocyte fouling on micro-engineered membranes', Biomedical Microdevices, vol. 20, no. 3, 55. https://doi.org/10.1007/s10544-018-0297-1

Erythrocyte fouling on micro-engineered membranes. / Amar, Levy I.; Guisado, Daniela; Faria, Monica; Jones, James P.; van Rijn, Cees J.M.; Hill, Michael I.; Leonard, Edward F.

In: Biomedical Microdevices, Vol. 20, No. 3, 55, 01.09.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Erythrocyte fouling on micro-engineered membranes

AU - Amar, Levy I.

AU - Guisado, Daniela

AU - Faria, Monica

AU - Jones, James P.

AU - van Rijn, Cees J.M.

AU - Hill, Michael I.

AU - Leonard, Edward F.

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Crossflow microfiltration of plasma from blood through microsieves in a microchannel is potentially useful in many biomedical applications, including clinically as a wearable water removal device under development by the authors. We report experiments that correlate filtration rates, transmembrane pressures (TMP) and shear rates during filtration through a microscopically high channel bounded by a low intrinsic resistance photolithographically-produced porous semiconductor membrane. These experiments allowed observation of erythrocyte behavior at the filtering surface and showed how their unique deformability properties dominated filtration resistance. At low filtration rates (corresponding to low TMP), they rolled along the filter surface, but at higher filtration rates (corresponding to higher TMP), they anchored themselves to the filter membrane, forming a self-assembled, incomplete monolayer. The incompleteness of the layer was an essential feature of the monolayer’s ability to support sustainable filtration. Maximum steady-state filtration flux was a function of wall shear rate, as predicted by conventional crossflow filtration theory, but, contrary to theories based on convective diffusion, showed weak dependence of filtration on erythrocyte concentration. Post-filtration scanning electron micrographs revealed significant capture and deformation of erythrocytes in all filter pores in the range 0.25 to 2 μm diameter. We report filtration rates through these filters and describe a largely unrecognized mechanism that allows stable filtration in the presence of substantial cell layers.

AB - Crossflow microfiltration of plasma from blood through microsieves in a microchannel is potentially useful in many biomedical applications, including clinically as a wearable water removal device under development by the authors. We report experiments that correlate filtration rates, transmembrane pressures (TMP) and shear rates during filtration through a microscopically high channel bounded by a low intrinsic resistance photolithographically-produced porous semiconductor membrane. These experiments allowed observation of erythrocyte behavior at the filtering surface and showed how their unique deformability properties dominated filtration resistance. At low filtration rates (corresponding to low TMP), they rolled along the filter surface, but at higher filtration rates (corresponding to higher TMP), they anchored themselves to the filter membrane, forming a self-assembled, incomplete monolayer. The incompleteness of the layer was an essential feature of the monolayer’s ability to support sustainable filtration. Maximum steady-state filtration flux was a function of wall shear rate, as predicted by conventional crossflow filtration theory, but, contrary to theories based on convective diffusion, showed weak dependence of filtration on erythrocyte concentration. Post-filtration scanning electron micrographs revealed significant capture and deformation of erythrocytes in all filter pores in the range 0.25 to 2 μm diameter. We report filtration rates through these filters and describe a largely unrecognized mechanism that allows stable filtration in the presence of substantial cell layers.

KW - Blood

KW - Cross-flow

KW - Erythrocytes

KW - Fouling

KW - Microfiltration model

KW - Microfluidics

KW - Microsieve

KW - Nanopores

KW - Photolithography

KW - Sieve

U2 - 10.1007/s10544-018-0297-1

DO - 10.1007/s10544-018-0297-1

M3 - Article

VL - 20

JO - Biomedical Microdevices

JF - Biomedical Microdevices

SN - 1387-2176

IS - 3

M1 - 55

ER -