Ercella succinigenes gen. nov., sp. nov., an anaerobic succinate-producing bacterium

A.H. van Gelder, D.Z. Sousa, W.I. Rijpstra, J.S. Damsté, A.J.M. Stams, I. Sanchez Andrea

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

A novel anaerobic succinate-producing bacterium, strain ZWBT, was isolated from sludge collected from a biogas desulfurization bioreactor (Eerbeek, the Netherlands). Cells were non-spore-forming, motile, slightly curved rods (0.4–0.5 µm in diameter and 2–3 µm in length), and stained Gram-negative. The temperature range for growth was 25–40 °C, with an optimum at 37 °C. The pH range for growth was 7.0–9.0, with an optimum at pH 7.5. Strain ZWBT was able to ferment glycerol and several carbohydrates mainly to H2, succinate and acetate. Sulfur and fumarate could be used as electron acceptors by strain ZWBT. The G+C content of the genomic DNA was 37.6 mol%. The most abundant fatty acids were iso-C14¿:¿0 and iso-C16¿:¿0 DMA. On the basis of 16S rRNA gene sequence similarity, strain ZWBT belongs to the family Ruminococcaceae and it is distantly related to Saccharofermentans acetigenes JCM 14006T (92.1¿%). Based on the physiological features and phylogenetic analysis, strain ZWBT represents a novel species of a new genus, for which the name Ercella succinigenes gen. nov., sp. nov. is proposed. The type strain of Ercella succinigenes is ZWBT (¿=¿DSM 27333T¿=¿JCM 19283T).
LanguageEnglish
Pages2449-2454
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
Issue number7
DOIs
Publication statusPublished - 2014

Fingerprint

Succinic Acid
succinic acid
Bacteria
Fumarates
strain analysis
bacterium
Biofuels
new species
bacteria
Base Composition
Bioreactors
Growth
Sewage
biogas
rRNA Genes
Sulfur
Netherlands
bioreactor
Glycerol
Names

Keywords

  • ribosomal-rna genes
  • acid
  • heterogeneity
  • fermentation
  • sequences
  • biofuels
  • glycerol
  • industry
  • operons
  • genomes

Cite this

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title = "Ercella succinigenes gen. nov., sp. nov., an anaerobic succinate-producing bacterium",
abstract = "A novel anaerobic succinate-producing bacterium, strain ZWBT, was isolated from sludge collected from a biogas desulfurization bioreactor (Eerbeek, the Netherlands). Cells were non-spore-forming, motile, slightly curved rods (0.4–0.5 µm in diameter and 2–3 µm in length), and stained Gram-negative. The temperature range for growth was 25–40 °C, with an optimum at 37 °C. The pH range for growth was 7.0–9.0, with an optimum at pH 7.5. Strain ZWBT was able to ferment glycerol and several carbohydrates mainly to H2, succinate and acetate. Sulfur and fumarate could be used as electron acceptors by strain ZWBT. The G+C content of the genomic DNA was 37.6 mol{\%}. The most abundant fatty acids were iso-C14¿:¿0 and iso-C16¿:¿0 DMA. On the basis of 16S rRNA gene sequence similarity, strain ZWBT belongs to the family Ruminococcaceae and it is distantly related to Saccharofermentans acetigenes JCM 14006T (92.1¿{\%}). Based on the physiological features and phylogenetic analysis, strain ZWBT represents a novel species of a new genus, for which the name Ercella succinigenes gen. nov., sp. nov. is proposed. The type strain of Ercella succinigenes is ZWBT (¿=¿DSM 27333T¿=¿JCM 19283T).",
keywords = "ribosomal-rna genes, acid, heterogeneity, fermentation, sequences, biofuels, glycerol, industry, operons, genomes",
author = "{van Gelder}, A.H. and D.Z. Sousa and W.I. Rijpstra and J.S. Damst{\'e} and A.J.M. Stams and {Sanchez Andrea}, I.",
year = "2014",
doi = "10.1099/ijs.0.058966-0",
language = "English",
volume = "64",
pages = "2449--2454",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Microbiology Society",
number = "7",

}

Ercella succinigenes gen. nov., sp. nov., an anaerobic succinate-producing bacterium. / van Gelder, A.H.; Sousa, D.Z.; Rijpstra, W.I.; Damsté, J.S.; Stams, A.J.M.; Sanchez Andrea, I.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, No. 7, 2014, p. 2449-2454.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Ercella succinigenes gen. nov., sp. nov., an anaerobic succinate-producing bacterium

AU - van Gelder, A.H.

AU - Sousa, D.Z.

AU - Rijpstra, W.I.

AU - Damsté, J.S.

AU - Stams, A.J.M.

AU - Sanchez Andrea, I.

PY - 2014

Y1 - 2014

N2 - A novel anaerobic succinate-producing bacterium, strain ZWBT, was isolated from sludge collected from a biogas desulfurization bioreactor (Eerbeek, the Netherlands). Cells were non-spore-forming, motile, slightly curved rods (0.4–0.5 µm in diameter and 2–3 µm in length), and stained Gram-negative. The temperature range for growth was 25–40 °C, with an optimum at 37 °C. The pH range for growth was 7.0–9.0, with an optimum at pH 7.5. Strain ZWBT was able to ferment glycerol and several carbohydrates mainly to H2, succinate and acetate. Sulfur and fumarate could be used as electron acceptors by strain ZWBT. The G+C content of the genomic DNA was 37.6 mol%. The most abundant fatty acids were iso-C14¿:¿0 and iso-C16¿:¿0 DMA. On the basis of 16S rRNA gene sequence similarity, strain ZWBT belongs to the family Ruminococcaceae and it is distantly related to Saccharofermentans acetigenes JCM 14006T (92.1¿%). Based on the physiological features and phylogenetic analysis, strain ZWBT represents a novel species of a new genus, for which the name Ercella succinigenes gen. nov., sp. nov. is proposed. The type strain of Ercella succinigenes is ZWBT (¿=¿DSM 27333T¿=¿JCM 19283T).

AB - A novel anaerobic succinate-producing bacterium, strain ZWBT, was isolated from sludge collected from a biogas desulfurization bioreactor (Eerbeek, the Netherlands). Cells were non-spore-forming, motile, slightly curved rods (0.4–0.5 µm in diameter and 2–3 µm in length), and stained Gram-negative. The temperature range for growth was 25–40 °C, with an optimum at 37 °C. The pH range for growth was 7.0–9.0, with an optimum at pH 7.5. Strain ZWBT was able to ferment glycerol and several carbohydrates mainly to H2, succinate and acetate. Sulfur and fumarate could be used as electron acceptors by strain ZWBT. The G+C content of the genomic DNA was 37.6 mol%. The most abundant fatty acids were iso-C14¿:¿0 and iso-C16¿:¿0 DMA. On the basis of 16S rRNA gene sequence similarity, strain ZWBT belongs to the family Ruminococcaceae and it is distantly related to Saccharofermentans acetigenes JCM 14006T (92.1¿%). Based on the physiological features and phylogenetic analysis, strain ZWBT represents a novel species of a new genus, for which the name Ercella succinigenes gen. nov., sp. nov. is proposed. The type strain of Ercella succinigenes is ZWBT (¿=¿DSM 27333T¿=¿JCM 19283T).

KW - ribosomal-rna genes

KW - acid

KW - heterogeneity

KW - fermentation

KW - sequences

KW - biofuels

KW - glycerol

KW - industry

KW - operons

KW - genomes

U2 - 10.1099/ijs.0.058966-0

DO - 10.1099/ijs.0.058966-0

M3 - Article

VL - 64

SP - 2449

EP - 2454

JO - International Journal of Systematic and Evolutionary Microbiology

T2 - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 7

ER -