Ercc1-/¿7 mice are a suitable model for ageing of mucosal immunity and microbiota

A.A. van Beek, B. Meijer, B. Sovran, P. de Vos, J.J.H. Hoeijmakers, P.J. Leenen, F. Hugenholtz, M.V. Boekschoten, H.F.J. Savelkoul

Research output: Contribution to conferenceAbstract

Abstract

As ageing profoundly affects the immune system, we investigated the effects of ageing on systemic and mucosal immunity, gut barrier and physiology, and microbiota composition. We compared the intestinal physiology and the mucosal immune system of young and old fast ageing Ercc1-/¿7 and wild-type (WT) mice. Multicolour flow cytometry was performed on several immune organs, and histology and FISH were performed on jejunum, ileum and colon of both Ercc1-/¿7 and WT mice. Gene expression arrays on ileum and colon and MIT-Chips were performed in Ercc1-/¿7 mice. In aged Ercc1-/¿7 and WT mice, a decrease in mucus thickness with bacteria touching the epithelium and leukocyte infiltration was observed both in ileum and colon. Multicolour flow cytometry showed several changes in immune status, including increased neutrophil and decreased lymphocyte frequencies in bone marrow and spleen. Gene expression arrays revealed upregulation of immune pathways in the ileum and colon of aged mice, such as pathways of granulocyte adhesion and diapedesis, and leukocyte extravastion. TGF-ß1, IFN-¿ and TNF were predicted to be more active (p
Original languageEnglish
Publication statusPublished - 2014
Event50th Anniversary Dutch Society for Immunology (NVVI), Kaatsheuvel NL -
Duration: 17 Dec 201419 Dec 2014

Conference

Conference50th Anniversary Dutch Society for Immunology (NVVI), Kaatsheuvel NL
Period17/12/1419/12/14

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