eQTL mapping of candidate genes for flower colour as a model for genetical genomics in Azalea

E. de Keyser, M.J.M. Smulders, E. van Bockstaele, J. de Riek

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademic

Abstract

Flower colour in azalea is inherited as a semi-qualitative trait and is mainly determined by differences in anthocyanins and flavonols. A two-gene model is used to explain the phenotypic variation between white, brick red and carmine red colour: W in case the flower petals contain anthocyanins and Q if flavonols are present as co-pigments. However, the regulatory network behind this pathway is still unclear. To identify key driver genes of the molecular pathway involved, a genetical genomics approach was followed. Genetics was combined with gene expression profiling, resulting in eQTL mapping. So far, mainly micro-array data have been used for this, limiting the technique to large scale projects. Nevertheless, qPCR can be a good and cost?efficient alternative, as we will demonstrate for flower colour in azalea. A genetic map of 13 linkage groups was constructed using a crossing population segregating for flower colour (250 plants). Besides anonymous AFLP and SSR markers also a set of functional markers
Original languageEnglish
Title of host publicationProceedings of the 19th Eucarpia General Congress, 21-24 May 2012, Budapest, Hungary
EditorsZ. Bedö, L. Láng
Pages102-105
Publication statusPublished - 2012
Event19th Eucarpia General Congress, Budapest, Hungary -
Duration: 21 May 201224 May 2012

Conference

Conference19th Eucarpia General Congress, Budapest, Hungary
Period21/05/1224/05/12

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