eQTL mapping of candidate genes for flower colour as a model for genetical genomics in Azalea

E. de Keyser, M.J.M. Smulders, E. van Bockstaele, J. de Riek

Research output: Chapter in Book/Report/Conference proceedingConference paper

Abstract

Flower colour in azalea is inherited as a semi-qualitative trait and is mainly determined by differences in anthocyanins and flavonols. A two-gene model is used to explain the phenotypic variation between white, brick red and carmine red colour: W in case the flower petals contain anthocyanins and Q if flavonols are present as co-pigments. However, the regulatory network behind this pathway is still unclear. To identify key driver genes of the molecular pathway involved, a genetical genomics approach was followed. Genetics was combined with gene expression profiling, resulting in eQTL mapping. So far, mainly micro-array data have been used for this, limiting the technique to large scale projects. Nevertheless, qPCR can be a good and cost?efficient alternative, as we will demonstrate for flower colour in azalea. A genetic map of 13 linkage groups was constructed using a crossing population segregating for flower colour (250 plants). Besides anonymous AFLP and SSR markers also a set of functional markers
Original languageEnglish
Title of host publicationProceedings of the 19th Eucarpia General Congress, 21-24 May 2012, Budapest, Hungary
EditorsZ. Bedö, L. Láng
Pages102-105
Publication statusPublished - 2012
Event19th Eucarpia General Congress, Budapest, Hungary -
Duration: 21 May 201224 May 2012

Conference

Conference19th Eucarpia General Congress, Budapest, Hungary
Period21/05/1224/05/12

Fingerprint

Rhododendron
chromosome mapping
flowers
genomics
color
flavonols
anthocyanins
bricks
linkage groups
phenotypic variation
corolla
amplified fragment length polymorphism
genes
pigments
gene expression
methodology

Cite this

de Keyser, E., Smulders, M. J. M., van Bockstaele, E., & de Riek, J. (2012). eQTL mapping of candidate genes for flower colour as a model for genetical genomics in Azalea. In Z. Bedö, & L. Láng (Eds.), Proceedings of the 19th Eucarpia General Congress, 21-24 May 2012, Budapest, Hungary (pp. 102-105)
de Keyser, E. ; Smulders, M.J.M. ; van Bockstaele, E. ; de Riek, J. / eQTL mapping of candidate genes for flower colour as a model for genetical genomics in Azalea. Proceedings of the 19th Eucarpia General Congress, 21-24 May 2012, Budapest, Hungary. editor / Z. Bedö ; L. Láng. 2012. pp. 102-105
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de Keyser, E, Smulders, MJM, van Bockstaele, E & de Riek, J 2012, eQTL mapping of candidate genes for flower colour as a model for genetical genomics in Azalea. in Z Bedö & L Láng (eds), Proceedings of the 19th Eucarpia General Congress, 21-24 May 2012, Budapest, Hungary. pp. 102-105, 19th Eucarpia General Congress, Budapest, Hungary, 21/05/12.

eQTL mapping of candidate genes for flower colour as a model for genetical genomics in Azalea. / de Keyser, E.; Smulders, M.J.M.; van Bockstaele, E.; de Riek, J.

Proceedings of the 19th Eucarpia General Congress, 21-24 May 2012, Budapest, Hungary. ed. / Z. Bedö; L. Láng. 2012. p. 102-105.

Research output: Chapter in Book/Report/Conference proceedingConference paper

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de Keyser E, Smulders MJM, van Bockstaele E, de Riek J. eQTL mapping of candidate genes for flower colour as a model for genetical genomics in Azalea. In Bedö Z, Láng L, editors, Proceedings of the 19th Eucarpia General Congress, 21-24 May 2012, Budapest, Hungary. 2012. p. 102-105