Epigenetic Approaches in Non-Model Plants

Teresa M. Boquete, Niels C.A.M. Wagemaker, Philippine Vergeer, Jeannie Mounger, Christina L. Richards*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

1 Citation (Scopus)

Abstract

Reduced representation bisulfite sequencing is an emerging methodology for evolutionary and ecological genomics and epigenomics research because it provides a cost-effective, high-resolution tool for exploration and comparative analysis of DNA methylation and genetic variation. Here we describe how digestion of genomic plant DNA with restriction enzymes, subsequent bisulfite conversion of unmethylated cytosines, and final DNA sequencing allow for the examination of genome-wide genetic and epigenetic variation in plants without the need for a reference genome. We explain how the use of several combinations of barcoded adapters for the creation of highly multiplexed libraries allows the inclusion of up to 144 different samples/individuals in only one sequencing lane.

Original languageEnglish
Title of host publicationPlant Epigenetics and Epigenomics
Place of PublicationNew York
PublisherHumana Press
Pages203-215
Number of pages13
Volume2093
ISBN (Electronic)9781071601792
ISBN (Print)9781071601785
DOIs
Publication statusPublished - 23 Feb 2020

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherSpringer Verlag
ISSN (Print)1064-3745

Keywords

  • Bisulfite treatment
  • DNA methylation
  • epiGBS
  • Epigenetics
  • Multiplexing
  • Next-generation sequencing
  • Reduced representation sequencing
  • Restriction enzymes

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