EPG monitoring of the probing behaviour of the common brown leafhopper Orosius orientalis on artificial diet and selected host plants

P. Trebicki, W.F. Tjallingii, R.M. Harding, B.C. Rodoni, K.S. Powell

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)

Abstract

The common brown leafhopper Orosius orientalis (Hemiptera: Cicadellidae) is a polyphagous vector of a range of economically important pathogens, including phytoplasmas and viruses, which infect a diverse range of crops. Studies on the plant penetration behaviour by O. orientalis were conducted using the electrical penetration graph (EPG) technique to assist in the characterisation of pathogen acquisition and transmission. EPG waveforms representing different probing activities were acquired from adult O. orientalis probing in planta, using two host species, tobacco Nicotiana tabacum and bean Phaseolus vulgaris, and in vitro using a simple sucrose-based artificial diet. Five waveforms (O1–O5) were evident when O. orientalis fed on bean, whereas only four waveforms (O1–O4) and three waveforms (O1–O3) were observed when the leafhopper fed on tobacco and on the artificial diet, respectively. Both the mean duration of each waveform and waveform type differed markedly depending on the food substrate. Waveform O4 was not observed on the artificial diet and occurred relatively rarely on tobacco plants when compared with bean plants. Waveform O5 was only observed with leafhoppers probing on beans. The attributes of the waveforms and comparative analyses with previously published Hemipteran data are presented and discussed, but further characterisation studies will be needed to confirm our suggestions.
Original languageEnglish
Pages (from-to)405-415
JournalArthropod-Plant Interactions
Volume6
Issue number3
DOIs
Publication statusPublished - 2012

Keywords

  • electrical penetration graphs
  • susceptible rice varieties
  • feeding-behavior
  • nilaparvata-lugens
  • southeastern australia
  • stylet penetration
  • xylella-fastidiosa
  • fine-structure
  • nymphal stages
  • aphids

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