Enrichment of ANME-1 from Eckernförde Bay sediment on thiosulfate, methane and short-chain fatty acids

C.G. Jagersma, R.J.W. Meulepas, P.H.A. Timmers, A. Szperl, P.N.L. Lens, A.J.M. Stams

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13 Citations (Scopus)

Abstract

The microorganisms involved in sulfate-dependent anaerobic oxidation of methane (AOM) have not yet been isolated. In an attempt to stimulate the growth of anaerobic methanotrophs and associated sulfate reducing bacteria (SRB), Eckernförde Bay sediment was incubated with different combinations of electron donors and acceptors. The organisms involved in AOM coupled to sulfate reduction (ANME-1, ANME-2, and Desulfosarcina/Desulfococcus) were monitored using specific primers and probes. With thiosulfate as sole electron acceptor and acetate, pyruvate or butyrate as the sole electron donor, ANME-1 became the dominant archaeal species. This finding suggests that ANME-1 archaea are not obligate methanotrophs and that ANME-1 can grow on acetate, pyruvate or butyrate
Original languageEnglish
Pages (from-to)482-489
JournalJournal of Biotechnology
Volume157
Issue number4
DOIs
Publication statusPublished - 2012

Keywords

  • sulfate-reducing bacteria
  • continuous-flow bioreactor
  • 16s ribosomal-rna
  • anaerobic oxidation
  • marine-sediments
  • methanotrophic archaea
  • microbial-communities
  • oxidizing archaea
  • electron-donor
  • seep sediments

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