Enhancing leaf photosynthesis from altered chlorophyll content requires optimal partitioning of nitrogen

Zhenxiang Zhou, Paul C. Struik, Junfei Gu, Peter E.L. van der Putten, Zhiqin Wang, Xinyou Yin*, Jianchang Yang*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)

Abstract

While optimising leaf chlorophyll content ([CHL]) has been proposed as a relevant means to manipulate canopy light penetration and canopy photosynthesis, effects of modifying [CHL] on leaf photosynthesis are yet to be investigated thoroughly. A greenhouse experiment and a field experiment were conducted involving rice genotypes of different genetic backgrounds and their leaf-colour variants. Leaf photosynthesis was more influenced by alteration to yellow-leaf than to stay-green cases. Higher specific leaf area and stomatal conductance were observed in two yellow-leaf variants, while only one yellow-leaf variant showed significantly increased Rubisco carboxylation capacity (Vcmax), maximum electron transport rate (Jmax), and photosynthetic nitrogen-use efficiency (PNUE). Model analysis indicated that reducing leaf [CHL] decreased the energy loss via non-photochemical quenching, but improving Vcmax, Jmax, and PNUE would require an improved nitrogen distribution pattern within the leaf. Label-free quantitative proteomics confirmed that an increased investment of nitrogen in Cyt b6/f and Rubisco was observed in the yellow-leaf variant of the genetic background with improved Vcmax, Jmax, and PNUE, but not in the other background. Our results suggest that reducing [CHL] can improve leaf photosynthesis only if the saved nitrogen is optimally distributed to proteins that are more rate-limiting to photosynthesis.

Original languageEnglish
Pages (from-to)24-36
Number of pages13
JournalCrop and Environment
Volume2
Issue number1
DOIs
Publication statusPublished - Mar 2023

Keywords

  • Chlorophyll content
  • Light energy utilisation efficiency
  • Nitrogen partitioning
  • Non-photochemical quenching
  • Oryza sativa
  • Photosynthetic capacity

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