Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA

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Abstract

Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.
Original languageEnglish
Pages (from-to)7817-7820
JournalApplied and Environmental Microbiology
Volume74
Issue number24
DOIs
Publication statusPublished - 2008

Keywords

  • restriction
  • thuringiensis
  • strains

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