Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA

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Abstract

Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.
Original languageEnglish
Pages (from-to)7817-7820
JournalApplied and Environmental Microbiology
Volume74
Issue number24
DOIs
Publication statusPublished - 2008

Fingerprint

Bacillus weihenstephanensis
Bacillus cereus
methylation
DNA Methylation
plasmid
Bacillus
plasmids
Plasmids
DNA Restriction-Modification Enzymes
DNA
Electroporation
electroporation
restriction endonucleases
digestion
Digestion
In Vitro Techniques

Keywords

  • restriction
  • thuringiensis
  • strains

Cite this

@article{c798dc45dd09490587c959e0342e1812,
title = "Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA",
abstract = "Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.",
keywords = "restriction, thuringiensis, strains",
author = "{Nierop Groot}, M.N. and F. Nieboer and T. Abee",
year = "2008",
doi = "10.1128/AEM.01932-08",
language = "English",
volume = "74",
pages = "7817--7820",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "24",

}

TY - JOUR

T1 - Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA

AU - Nierop Groot, M.N.

AU - Nieboer, F.

AU - Abee, T.

PY - 2008

Y1 - 2008

N2 - Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.

AB - Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.

KW - restriction

KW - thuringiensis

KW - strains

U2 - 10.1128/AEM.01932-08

DO - 10.1128/AEM.01932-08

M3 - Article

VL - 74

SP - 7817

EP - 7820

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 24

ER -