Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA

M.N. Nierop Groot; F. Nieboer; T. Abee

doi:10.1128/AEM.01932-08

Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA

M.N. Nierop Groot, F. Nieboer, T. Abee

Research output: Contribution to journal › Article › Academic › peer-review

13 Citations (Scopus)

Abstract

Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.

Original language	English
Pages (from-to)	7817-7820
Journal	Applied and Environmental Microbiology
Volume	74
Issue number	24
DOIs	https://doi.org/10.1128/AEM.01932-08
Publication status	Published - 2008

Keywords

restriction
thuringiensis
strains

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10.1128/AEM.01932-08

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title = "Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA",

abstract = "Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.",

keywords = "restriction, thuringiensis, strains",

author = "{Nierop Groot}, M.N. and F. Nieboer and T. Abee",

year = "2008",

doi = "10.1128/AEM.01932-08",

language = "English",

volume = "74",

pages = "7817--7820",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

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T1 - Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA

AU - Nierop Groot, M.N.

AU - Nieboer, F.

AU - Abee, T.

PY - 2008

Y1 - 2008

N2 - Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.

AB - Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.

KW - restriction

KW - thuringiensis

KW - strains

U2 - 10.1128/AEM.01932-08

DO - 10.1128/AEM.01932-08

M3 - Article

VL - 74

SP - 7817

EP - 7820

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 24

ER -