Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 7817-7820 |
| Journal | Applied and Environmental Microbiology |
| Volume | 74 |
| Issue number | 24 |
| DOIs | |
| Publication status | Published - 2008 |
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Keywords
- restriction
- thuringiensis
- strains
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Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA. / Nierop Groot, M.N.; Nieboer, F.; Abee, T.
In: Applied and Environmental Microbiology, Vol. 74, No. 24, 2008, p. 7817-7820.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Enhanced transformation efficiency of recalcitrant Bacillus cereus and Bacillus weihenstephanensis isolates upon in vitro methylation of plasmid DNA
AU - Nierop Groot, M.N.
AU - Nieboer, F.
AU - Abee, T.
PY - 2008
Y1 - 2008
N2 - Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.
AB - Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.
KW - restriction
KW - thuringiensis
KW - strains
U2 - 10.1128/AEM.01932-08
DO - 10.1128/AEM.01932-08
M3 - Article
VL - 74
SP - 7817
EP - 7820
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 24
ER -