Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana

C. Safi*, L. Cabas Rodriguez, W.J. Mulder, N. Engelen-Smit, W. Spekking, L.A.M. van den Broek, G. Olivieri, L. Sijtsma

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)

Abstract

Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95% cell disintegration, ±50% (w/w) release of total proteins and low energy input (<0.5 kWh.kg−1 biomass). Enzymatic treatment required low energy input (<0.34 kWh.kg−1 biomass), but it only released ±35% protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10.44 kWh.kg−1 biomass) nor successful for protein release (only 10% proteins w/w) and cell disintegration. The release of proteins after applying HPH and bead milling always required less intensive operating conditions for cell disruption. The energy cost per unit of released protein ranged from 0.15–0.25 €.kgProtein −1 in case of HPH, and up to 2–20 €.kgProtein −1 in case of PEF.
Original languageEnglish
Pages (from-to)204-210
JournalBioresource Technology
Volume239
DOIs
Publication statusPublished - 2017

Fingerprint

Energy utilization
Cells
Proteins
protein
Water
Disintegration
Biomass
energy
water
electric field
biomass
Electric fields
energy consumption
cost
Costs

Keywords

  • Bead milling
  • Enzymes
  • High-pressure homogenization
  • Pulsed Electric Field
  • Soluble proteins

Cite this

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title = "Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana",
abstract = "Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95{\%} cell disintegration, ±50{\%} (w/w) release of total proteins and low energy input (<0.5 kWh.kg−1 biomass). Enzymatic treatment required low energy input (<0.34 kWh.kg−1 biomass), but it only released ±35{\%} protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10.44 kWh.kg−1 biomass) nor successful for protein release (only 10{\%} proteins w/w) and cell disintegration. The release of proteins after applying HPH and bead milling always required less intensive operating conditions for cell disruption. The energy cost per unit of released protein ranged from 0.15–0.25 €.kgProtein −1 in case of HPH, and up to 2–20 €.kgProtein −1 in case of PEF.",
keywords = "Bead milling, Enzymes, High-pressure homogenization, Pulsed Electric Field, Soluble proteins",
author = "C. Safi and {Cabas Rodriguez}, L. and W.J. Mulder and N. Engelen-Smit and W. Spekking and {van den Broek}, L.A.M. and G. Olivieri and L. Sijtsma",
year = "2017",
doi = "10.1016/j.biortech.2017.05.012",
language = "English",
volume = "239",
pages = "204--210",
journal = "Bioresource Technology",
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Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana. / Safi, C.; Cabas Rodriguez, L.; Mulder, W.J.; Engelen-Smit, N.; Spekking, W.; van den Broek, L.A.M.; Olivieri, G.; Sijtsma, L.

In: Bioresource Technology, Vol. 239, 2017, p. 204-210.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana

AU - Safi, C.

AU - Cabas Rodriguez, L.

AU - Mulder, W.J.

AU - Engelen-Smit, N.

AU - Spekking, W.

AU - van den Broek, L.A.M.

AU - Olivieri, G.

AU - Sijtsma, L.

PY - 2017

Y1 - 2017

N2 - Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95% cell disintegration, ±50% (w/w) release of total proteins and low energy input (<0.5 kWh.kg−1 biomass). Enzymatic treatment required low energy input (<0.34 kWh.kg−1 biomass), but it only released ±35% protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10.44 kWh.kg−1 biomass) nor successful for protein release (only 10% proteins w/w) and cell disintegration. The release of proteins after applying HPH and bead milling always required less intensive operating conditions for cell disruption. The energy cost per unit of released protein ranged from 0.15–0.25 €.kgProtein −1 in case of HPH, and up to 2–20 €.kgProtein −1 in case of PEF.

AB - Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95% cell disintegration, ±50% (w/w) release of total proteins and low energy input (<0.5 kWh.kg−1 biomass). Enzymatic treatment required low energy input (<0.34 kWh.kg−1 biomass), but it only released ±35% protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10.44 kWh.kg−1 biomass) nor successful for protein release (only 10% proteins w/w) and cell disintegration. The release of proteins after applying HPH and bead milling always required less intensive operating conditions for cell disruption. The energy cost per unit of released protein ranged from 0.15–0.25 €.kgProtein −1 in case of HPH, and up to 2–20 €.kgProtein −1 in case of PEF.

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