TY - JOUR
T1 - Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana
AU - Safi, C.
AU - Cabas Rodriguez, L.
AU - Mulder, W.J.
AU - Engelen-Smit, N.
AU - Spekking, W.
AU - van den Broek, L.A.M.
AU - Olivieri, G.
AU - Sijtsma, L.
PY - 2017
Y1 - 2017
N2 - Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95% cell disintegration, ±50% (w/w) release of total proteins and low energy input (<0.5 kWh.kg−1 biomass). Enzymatic treatment required low energy input (<0.34 kWh.kg−1 biomass), but it only released ±35% protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10.44 kWh.kg−1 biomass) nor successful for protein release (only 10% proteins w/w) and cell disintegration. The release of proteins after applying HPH and bead milling always required less intensive operating conditions for cell disruption. The energy cost per unit of released protein ranged from 0.15–0.25 €.kgProtein −1 in case of HPH, and up to 2–20 €.kgProtein −1 in case of PEF.
AB - Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95% cell disintegration, ±50% (w/w) release of total proteins and low energy input (<0.5 kWh.kg−1 biomass). Enzymatic treatment required low energy input (<0.34 kWh.kg−1 biomass), but it only released ±35% protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10.44 kWh.kg−1 biomass) nor successful for protein release (only 10% proteins w/w) and cell disintegration. The release of proteins after applying HPH and bead milling always required less intensive operating conditions for cell disruption. The energy cost per unit of released protein ranged from 0.15–0.25 €.kgProtein −1 in case of HPH, and up to 2–20 €.kgProtein −1 in case of PEF.
KW - Bead milling
KW - Enzymes
KW - High-pressure homogenization
KW - Pulsed Electric Field
KW - Soluble proteins
U2 - 10.1016/j.biortech.2017.05.012
DO - 10.1016/j.biortech.2017.05.012
M3 - Article
AN - SCOPUS:85019270194
SN - 0960-8524
VL - 239
SP - 204
EP - 210
JO - Bioresource Technology
JF - Bioresource Technology
ER -
