Endurance exercise increases intestinal uptake of the peanut allergen Ara h 6 after peanut consumption in humans

Lonneke M. Janssen Duijghuijsen, Klaske Van Norren, Sander Grefte, Stef J. Koppelman, Kaatje Lenaerts, Jaap Keijer, Renger F. Witkamp, Harry J. Wichers

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Controlled studies on the effect of exercise on intestinal uptake of protein are scarce and underlying mechanisms largely unclear. We studied the uptake of the major allergen Ara h 6 following peanut consumption in an exercise model and compared this with changes in markers of intestinal permeability and integrity. Ten overnight-fasted healthy non-allergic men (n = 4) and women (n = 6) (23 ± 4 years) ingested 100 g of peanuts together with a lactulose/rhamnose (L/R) solution, followed by rest or by 60 min cycling at 70% of their maximal workload. Significantly higher, though variable, levels of Ara h 6 in serum were found during exercise compared to rest (Peak p = 0.03, area under the curve p = 0.006), with individual fold changes ranging from no increase to an increase of over 150-fold in the uptake of Ara h 6. Similarly, uptake of lactulose (2-18 fold change, p = 0.0009) and L/R ratios (0.4-7.9 fold change, p = 0.04) were significantly increased which indicates an increase in intestinal permeability. Intestinal permeability and uptake of Ara h 6 were strongly correlated (r = 0.77, p <0.0001 for lactulose and Ara h 6). Endurance exercise after consumption may lead to increased paracellular intestinal uptake of food proteins.

LanguageEnglish
Article number84
JournalNutrients
Volume9
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

Lactulose
allergens
peanuts
lactulose
exercise
Exercise
uptake mechanisms
Rhamnose
Permeability
permeability
rhamnose
Workload
Area Under Curve
Proteins
Food
protein sources
Arachis hypogaea Ara h 6 allergen
Arachis
Serum

Keywords

  • Allergen
  • Ara h 6
  • Endurance exercise
  • Food-dependent exercise-induced anaphylaxis
  • Intestinal permeability

Cite this

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title = "Endurance exercise increases intestinal uptake of the peanut allergen Ara h 6 after peanut consumption in humans",
abstract = "Controlled studies on the effect of exercise on intestinal uptake of protein are scarce and underlying mechanisms largely unclear. We studied the uptake of the major allergen Ara h 6 following peanut consumption in an exercise model and compared this with changes in markers of intestinal permeability and integrity. Ten overnight-fasted healthy non-allergic men (n = 4) and women (n = 6) (23 ± 4 years) ingested 100 g of peanuts together with a lactulose/rhamnose (L/R) solution, followed by rest or by 60 min cycling at 70{\%} of their maximal workload. Significantly higher, though variable, levels of Ara h 6 in serum were found during exercise compared to rest (Peak p = 0.03, area under the curve p = 0.006), with individual fold changes ranging from no increase to an increase of over 150-fold in the uptake of Ara h 6. Similarly, uptake of lactulose (2-18 fold change, p = 0.0009) and L/R ratios (0.4-7.9 fold change, p = 0.04) were significantly increased which indicates an increase in intestinal permeability. Intestinal permeability and uptake of Ara h 6 were strongly correlated (r = 0.77, p <0.0001 for lactulose and Ara h 6). Endurance exercise after consumption may lead to increased paracellular intestinal uptake of food proteins.",
keywords = "Allergen, Ara h 6, Endurance exercise, Food-dependent exercise-induced anaphylaxis, Intestinal permeability",
author = "{Janssen Duijghuijsen}, {Lonneke M.} and {Van Norren}, Klaske and Sander Grefte and Koppelman, {Stef J.} and Kaatje Lenaerts and Jaap Keijer and Witkamp, {Renger F.} and Wichers, {Harry J.}",
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Endurance exercise increases intestinal uptake of the peanut allergen Ara h 6 after peanut consumption in humans. / Janssen Duijghuijsen, Lonneke M.; Van Norren, Klaske; Grefte, Sander; Koppelman, Stef J.; Lenaerts, Kaatje; Keijer, Jaap; Witkamp, Renger F.; Wichers, Harry J.

In: Nutrients, Vol. 9, No. 1, 84, 2017.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Endurance exercise increases intestinal uptake of the peanut allergen Ara h 6 after peanut consumption in humans

AU - Janssen Duijghuijsen, Lonneke M.

AU - Van Norren, Klaske

AU - Grefte, Sander

AU - Koppelman, Stef J.

AU - Lenaerts, Kaatje

AU - Keijer, Jaap

AU - Witkamp, Renger F.

AU - Wichers, Harry J.

PY - 2017

Y1 - 2017

N2 - Controlled studies on the effect of exercise on intestinal uptake of protein are scarce and underlying mechanisms largely unclear. We studied the uptake of the major allergen Ara h 6 following peanut consumption in an exercise model and compared this with changes in markers of intestinal permeability and integrity. Ten overnight-fasted healthy non-allergic men (n = 4) and women (n = 6) (23 ± 4 years) ingested 100 g of peanuts together with a lactulose/rhamnose (L/R) solution, followed by rest or by 60 min cycling at 70% of their maximal workload. Significantly higher, though variable, levels of Ara h 6 in serum were found during exercise compared to rest (Peak p = 0.03, area under the curve p = 0.006), with individual fold changes ranging from no increase to an increase of over 150-fold in the uptake of Ara h 6. Similarly, uptake of lactulose (2-18 fold change, p = 0.0009) and L/R ratios (0.4-7.9 fold change, p = 0.04) were significantly increased which indicates an increase in intestinal permeability. Intestinal permeability and uptake of Ara h 6 were strongly correlated (r = 0.77, p <0.0001 for lactulose and Ara h 6). Endurance exercise after consumption may lead to increased paracellular intestinal uptake of food proteins.

AB - Controlled studies on the effect of exercise on intestinal uptake of protein are scarce and underlying mechanisms largely unclear. We studied the uptake of the major allergen Ara h 6 following peanut consumption in an exercise model and compared this with changes in markers of intestinal permeability and integrity. Ten overnight-fasted healthy non-allergic men (n = 4) and women (n = 6) (23 ± 4 years) ingested 100 g of peanuts together with a lactulose/rhamnose (L/R) solution, followed by rest or by 60 min cycling at 70% of their maximal workload. Significantly higher, though variable, levels of Ara h 6 in serum were found during exercise compared to rest (Peak p = 0.03, area under the curve p = 0.006), with individual fold changes ranging from no increase to an increase of over 150-fold in the uptake of Ara h 6. Similarly, uptake of lactulose (2-18 fold change, p = 0.0009) and L/R ratios (0.4-7.9 fold change, p = 0.04) were significantly increased which indicates an increase in intestinal permeability. Intestinal permeability and uptake of Ara h 6 were strongly correlated (r = 0.77, p <0.0001 for lactulose and Ara h 6). Endurance exercise after consumption may lead to increased paracellular intestinal uptake of food proteins.

KW - Allergen

KW - Ara h 6

KW - Endurance exercise

KW - Food-dependent exercise-induced anaphylaxis

KW - Intestinal permeability

U2 - 10.3390/nu9010084

DO - 10.3390/nu9010084

M3 - Article

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JO - Nutrients

T2 - Nutrients

JF - Nutrients

SN - 2072-6643

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ER -