Endurance exercise increases intestinal uptake of the peanut allergen Ara h 6 after peanut consumption in humans

Lonneke M. Janssen Duijghuijsen, Klaske Van Norren, Sander Grefte, Stef J. Koppelman, Kaatje Lenaerts, Jaap Keijer, Renger F. Witkamp, Harry J. Wichers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)


Controlled studies on the effect of exercise on intestinal uptake of protein are scarce and underlying mechanisms largely unclear. We studied the uptake of the major allergen Ara h 6 following peanut consumption in an exercise model and compared this with changes in markers of intestinal permeability and integrity. Ten overnight-fasted healthy non-allergic men (n = 4) and women (n = 6) (23 ± 4 years) ingested 100 g of peanuts together with a lactulose/rhamnose (L/R) solution, followed by rest or by 60 min cycling at 70% of their maximal workload. Significantly higher, though variable, levels of Ara h 6 in serum were found during exercise compared to rest (Peak p = 0.03, area under the curve p = 0.006), with individual fold changes ranging from no increase to an increase of over 150-fold in the uptake of Ara h 6. Similarly, uptake of lactulose (2-18 fold change, p = 0.0009) and L/R ratios (0.4-7.9 fold change, p = 0.04) were significantly increased which indicates an increase in intestinal permeability. Intestinal permeability and uptake of Ara h 6 were strongly correlated (r = 0.77, p <0.0001 for lactulose and Ara h 6). Endurance exercise after consumption may lead to increased paracellular intestinal uptake of food proteins.

Original languageEnglish
Article number84
Issue number1
Publication statusPublished - 2017


  • Allergen
  • Ara h 6
  • Endurance exercise
  • Food-dependent exercise-induced anaphylaxis
  • Intestinal permeability


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