Endocytic and Secretory Traffic in Arabidopsis Merge in the Trans-Golgi Network/Early Endosome, an Independent and Highly Dynamic Organelle

C. Viotti, J. Bubeck, Y.D. Stierhof, M. Krebs, M. Langhans, W.A.M. van den Berg, W.M.A.M. van Dongen, S. Richter, N. Geldner, J. Takano, G. Juergens, S.C. de Vries, D.G. Robinson, K. Schumacher

Research output: Contribution to journalArticleAcademicpeer-review

292 Citations (Scopus)

Abstract

Plants constantly adjust their repertoire of plasma membrane proteins that mediates transduction of environmental and developmental signals as well as transport of ions, nutrients, and hormones. The importance of regulated secretory and endocytic trafficking is becoming increasingly clear; however, our knowledge of the compartments and molecular machinery involved is still fragmentary. We used immunogold electron microscopy and confocal laser scanning microscopy to trace the route of cargo molecules, including the BRASSINOSTEROID INSENSITIVE1 receptor and the REQUIRES HIGH BORON1 boron exporter, throughout the plant endomembrane system. Our results provide evidence that both endocytic and secretory cargo pass through the trans-Golgi network/early endosome (TGN/EE) and demonstrate that cargo in late endosomes/multivesicular bodies is destined for vacuolar degradation. Moreover, using spinning disc microscopy, we show that TGN/EEs move independently and are only transiently associated with an individual Golgi stack.
Original languageEnglish
Pages (from-to)1344-1357
JournalThe Plant Cell
Volume22
Issue number4
DOIs
Publication statusPublished - 2010

Keywords

  • cell-wall pectins
  • endoplasmic-reticulum
  • plant-cells
  • root-cells
  • prevacuolar compartments
  • boron transporter
  • homotypic fusion
  • atpase activity
  • brefeldin-a
  • by-2 cells

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