Abstract
A cDNA library of Aspergillus tubingensis was constructed in the yeast Kluyveromyces lactis, using a carbon source rich in xylogalacturonan. The library was screened using a hairy regions preparation from apple, and xylogalacturonan prepared from gum tragacanth as substrates. A novel endo-xylogalacturonase was found, XGH. The enzyme specifically degrades xylose-substituted galacturonic acid backbones. In lab scale filtration experiments, XGH was able to decrease membrane fouling caused by hairy regions from apple
Original language | English |
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Title of host publication | Advances in pectin and pectinase research |
Editors | A.G.J. Voragen, H.A. Schols, R.G.F. Visser |
Place of Publication | Dordrecht |
Pages | 257-266 |
Publication status | Published - 2003 |
Keywords
- pectins
- polygalacturonase
- enzymes
- apple juice
- aspergillus