Abstract
A cDNA library of Aspergillus tubingensis was constructed in the yeast Kluyveromyces lactis, using a carbon source rich in xylogalacturonan. The library was screened using a hairy regions preparation from apple, and xylogalacturonan prepared from gum tragacanth as substrates. A novel endo-xylogalacturonase was found, XGH. The enzyme specifically degrades xylose-substituted galacturonic acid backbones. In lab scale filtration experiments, XGH was able to decrease membrane fouling caused by hairy regions from apple
| Original language | English |
|---|---|
| Title of host publication | Advances in pectin and pectinase research |
| Editors | A.G.J. Voragen, H.A. Schols, R.G.F. Visser |
| Place of Publication | Dordrecht |
| Pages | 257-266 |
| Publication status | Published - 2003 |
Keywords
- pectins
- polygalacturonase
- enzymes
- apple juice
- aspergillus
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Herweijer, M. A., Vincken, J. P., Meeuwsen, P. J. A., van der Vlugt-Bergmans, C. J. B., Beldman, G., van Ooyen, A. J. J., & Voragen, A. G. J. (2003). Endo-xylogalacturonan hydrolase. In A. G. J. Voragen, H. A. Schols, & R. G. F. Visser (Eds.), Advances in pectin and pectinase research (pp. 257-266).