Endo-xylogalacturonan hydrolase

M.A. Herweijer, J.P. Vincken, P.J.A. Meeuwsen, C.J.B. van der Vlugt-Bergmans, G. Beldman, A.J.J. van Ooyen, A.G.J. Voragen

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A cDNA library of Aspergillus tubingensis was constructed in the yeast Kluyveromyces lactis, using a carbon source rich in xylogalacturonan. The library was screened using a hairy regions preparation from apple, and xylogalacturonan prepared from gum tragacanth as substrates. A novel endo-xylogalacturonase was found, XGH. The enzyme specifically degrades xylose-substituted galacturonic acid backbones. In lab scale filtration experiments, XGH was able to decrease membrane fouling caused by hairy regions from apple
Original languageEnglish
Title of host publicationAdvances in pectin and pectinase research
EditorsA.G.J. Voragen, H.A. Schols, R.G.F. Visser
Place of PublicationDordrecht
Pages257-266
Publication statusPublished - 2003

Keywords

  • pectins
  • polygalacturonase
  • enzymes
  • apple juice
  • aspergillus

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    Herweijer, M. A., Vincken, J. P., Meeuwsen, P. J. A., van der Vlugt-Bergmans, C. J. B., Beldman, G., van Ooyen, A. J. J., & Voragen, A. G. J. (2003). Endo-xylogalacturonan hydrolase. In A. G. J. Voragen, H. A. Schols, & R. G. F. Visser (Eds.), Advances in pectin and pectinase research (pp. 257-266).