A cDNA library of Aspergillus tubingensis was constructed in the yeast Kluyveromyces lactis, using a carbon source rich in xylogalacturonan. The library was screened using a hairy regions preparation from apple, and xylogalacturonan prepared from gum tragacanth as substrates. A novel endo-xylogalacturonase was found, XGH. The enzyme specifically degrades xylose-substituted galacturonic acid backbones. In lab scale filtration experiments, XGH was able to decrease membrane fouling caused by hairy regions from apple
|Title of host publication||Advances in pectin and pectinase research|
|Editors||A.G.J. Voragen, H.A. Schols, R.G.F. Visser|
|Place of Publication||Dordrecht|
|Publication status||Published - 2003|
- apple juice
Herweijer, M. A., Vincken, J. P., Meeuwsen, P. J. A., van der Vlugt-Bergmans, C. J. B., Beldman, G., van Ooyen, A. J. J., & Voragen, A. G. J. (2003). Endo-xylogalacturonan hydrolase. In A. G. J. Voragen, H. A. Schols, & R. G. F. Visser (Eds.), Advances in pectin and pectinase research (pp. 257-266).