Endo-xylogalacturonan hydrolase

M.A. Herweijer; J.P. Vincken; P.J.A. Meeuwsen; C.J.B. van der Vlugt-Bergmans; G. Beldman; A.J.J. van Ooyen; A.G.J. Voragen

Endo-xylogalacturonan hydrolase

M.A. Herweijer, J.P. Vincken, P.J.A. Meeuwsen, C.J.B. van der Vlugt-Bergmans, G. Beldman, A.J.J. van Ooyen, A.G.J. Voragen

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

Abstract

A cDNA library of Aspergillus tubingensis was constructed in the yeast Kluyveromyces lactis, using a carbon source rich in xylogalacturonan. The library was screened using a hairy regions preparation from apple, and xylogalacturonan prepared from gum tragacanth as substrates. A novel endo-xylogalacturonase was found, XGH. The enzyme specifically degrades xylose-substituted galacturonic acid backbones. In lab scale filtration experiments, XGH was able to decrease membrane fouling caused by hairy regions from apple

Original language	English
Title of host publication	Advances in pectin and pectinase research
Editors	A.G.J. Voragen, H.A. Schols, R.G.F. Visser
Place of Publication	Dordrecht
Pages	257-266
Publication status	Published - 2003

Keywords

pectins
polygalacturonase
enzymes
apple juice
aspergillus

Cite this

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title = "Endo-xylogalacturonan hydrolase",

abstract = "A cDNA library of Aspergillus tubingensis was constructed in the yeast Kluyveromyces lactis, using a carbon source rich in xylogalacturonan. The library was screened using a hairy regions preparation from apple, and xylogalacturonan prepared from gum tragacanth as substrates. A novel endo-xylogalacturonase was found, XGH. The enzyme specifically degrades xylose-substituted galacturonic acid backbones. In lab scale filtration experiments, XGH was able to decrease membrane fouling caused by hairy regions from apple",

keywords = "pectinen, polygalacturonase, enzymen, appelsap, aspergillus, pectins, polygalacturonase, enzymes, apple juice, aspergillus",

author = "M.A. Herweijer and J.P. Vincken and P.J.A. Meeuwsen and {van der Vlugt-Bergmans}, C.J.B. and G. Beldman and {van Ooyen}, A.J.J. and A.G.J. Voragen",

year = "2003",

language = "English",

isbn = "9781402011443",

pages = "257--266",

editor = "A.G.J. Voragen and H.A. Schols and R.G.F. Visser",

booktitle = "Advances in pectin and pectinase research",

}

TY - CHAP

T1 - Endo-xylogalacturonan hydrolase

AU - Herweijer, M.A.

AU - Vincken, J.P.

AU - Meeuwsen, P.J.A.

AU - van der Vlugt-Bergmans, C.J.B.

AU - Beldman, G.

AU - van Ooyen, A.J.J.

AU - Voragen, A.G.J.

PY - 2003

Y1 - 2003

N2 - A cDNA library of Aspergillus tubingensis was constructed in the yeast Kluyveromyces lactis, using a carbon source rich in xylogalacturonan. The library was screened using a hairy regions preparation from apple, and xylogalacturonan prepared from gum tragacanth as substrates. A novel endo-xylogalacturonase was found, XGH. The enzyme specifically degrades xylose-substituted galacturonic acid backbones. In lab scale filtration experiments, XGH was able to decrease membrane fouling caused by hairy regions from apple

AB - A cDNA library of Aspergillus tubingensis was constructed in the yeast Kluyveromyces lactis, using a carbon source rich in xylogalacturonan. The library was screened using a hairy regions preparation from apple, and xylogalacturonan prepared from gum tragacanth as substrates. A novel endo-xylogalacturonase was found, XGH. The enzyme specifically degrades xylose-substituted galacturonic acid backbones. In lab scale filtration experiments, XGH was able to decrease membrane fouling caused by hairy regions from apple

KW - pectinen

KW - polygalacturonase

KW - enzymen

KW - appelsap

KW - aspergillus

KW - pectins

KW - polygalacturonase

KW - enzymes

KW - apple juice

KW - aspergillus

M3 - Chapter

SN - 9781402011443

SP - 257

EP - 266

BT - Advances in pectin and pectinase research

A2 - Voragen, A.G.J.

A2 - Schols, H.A.

A2 - Visser, R.G.F.

CY - Dordrecht

ER -

Endo-xylogalacturonan hydrolase

Abstract

Keywords

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