Endo-glucanase digestion of oat β-Glucan enhances Dectin-1 activation in human dendritic cells

N.M. Sahasrabudhe, Lingmin Tian, Marco van den Berg, Geert Bruggeman, Erik Bruininx, H.A. Schols, M.M. Faas, Paul de Vos

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)


Oat β-Glucans were studied for their immunological impact before and after enzymatic digestion in order to enhance the efficacy of oat β-Glucans for application in functional foods. Oat β-Glucan is reported to have minimal impact compared to its fungal counterpart in vitro. Digestion with endo-glucanase enhanced its efficacy towards stimulating MCP-1, RANTES, IL-8, and IL-production in human dendritic cells as compared to the nondigested β-Glucan. This effect resulted from an enhanced activation of the Dectin-receptor. Our data suggest that the immune-stimulation was dependent on the β-(1-3) linkages and the reduced particle size of digested β-Glucans. Thus, we show that enzymatic pre-digestion of dietary fibres such as oat β-Glucan enhances its impact on specific immune receptors. We also demonstrate that particle size and/or molecular weight of oat β-Glucans and exposure of specific binding sites for the receptors might be important tools for designing efficacious functional feed and food additives.

Original languageEnglish
Pages (from-to)104-112
JournalJournal of Functional Foods
Publication statusPublished - 2016


  • Dectin-1
  • Dendritic cells
  • Endo-glucanase
  • Oat
  • Particle size
  • β-(1-3, 1-4) Glucan

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