Endo-1,3(4)-β-glucanase-treatment of oat β-glucan enhances fermentability by infant fecal microbiota, stimulates dectin-1 activation and attenuates inflammatory responses in immature dendritic cells

Renate Akkerman*, Madelon J. Logtenberg, Ran An, Marco A. Van Den Berg, Bart J. de Haan, Marijke M. Faas, Erwin Zoetendal, Paul de Vos, Henk A. Schols

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background: Non-digestible carbohydrates are added to infant formula to mimic the effects of human milk oligosaccharide by acting as prebiotics and stimulating the immune system. Although not yet used in infant formulas, β-glucans are known to have beneficial health effects, and are therefore of potential interest for supplementation. Methods and results: We investigated the in vitro fermentation of native and endo-1,3(4)-β-glucanase-treated oat β-glucan using pooled fecal inocula of 2-and 8-week-old infants. While native oat β-glucan was not utilized, both inocula specifically utilized oat β-glucan oligomers containing β(1→4)-linkages formed upon enzyme treatment. The fermentation rate was highest in the fecal microbiota of 2-week-old infants, and correlated with a high lactate production. Fermentation of media supplemented with native and enzyme-treated oat β-glucans increased the relative abundance of Enterococcus and attenuated proinflammatory cytokine production (IL-1β, IL-6, TNFα) in immature dendritic cells. This attenuating effect was more pronounced after enzyme treatment. This attenuation might result from the enhanced ability of fermented oat β-glucan to stimulate Dectin-1 receptors. Conclusion: Our findings demonstrate that endo-1,3(4)-β-glucanase treatment enhances the fermentability of oat β-glucan and attenuates pro-inflammatory responses. Hence, this study shows that especially enzyme-treated oat β-glucans have a high potential for supplementation of infant formula.

Original languageEnglish
Article number1660
JournalNutrients
Volume12
Issue number6
DOIs
Publication statusPublished - 3 Jun 2020

Keywords

  • Cytokine production
  • Dendritic cells
  • In vitro fermentation
  • Infant formula
  • Microbiota
  • Oat β-Glucan

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