Embryogenesis from isolated microspores of tulip : towards developing F1 hybrid varieties

J.B.M. Custers; E. Ennik; W. Eikelboom; J.J.M. Dons; M.M. van Lookeren Campagne

doi:10.17660/ActaHortic.1997.430.38

Embryogenesis from isolated microspores of tulip : towards developing F1 hybrid varieties

J.B.M. Custers, E. Ennik, W. Eikelboom, J.J.M. Dons, M.M. van Lookeren Campagne

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Scopus)

Abstract

This report describes advances in the technique of embryogenesis from tulip microspores in culture. High temperature pretreatment (32°C) of bulbs, which contain fully developed inflorescences, had a positive effect on the production of microspore embryos. The pretreatment shortened the time for embryo formation and stimulated the progression from the multicellular microspore stage into the embryo proper. Early unicellular microspores were found to be better starting material for culture than mid unicellular microspores. The technique of microspore embryogenesis was applicable to various tulip cultivars. The improved protocol now allows the production of large numbers of microspore-derived plants, and the further evaluation of doubled haploid plant technology for tulip.

Original language	Undefined/Unknown
Pages (from-to)	259-266
Journal	Acta Horticulturae
Volume	430
DOIs	https://doi.org/10.17660/ActaHortic.1997.430.38
Publication status	Published - 1997

Keywords

Doubled haploid
Homozygous
Tulipa

Access to Document

10.17660/ActaHortic.1997.430.38

Cite this

@article{981cd15260c54b50bb7c507e0127c4e4,

title = "Embryogenesis from isolated microspores of tulip : towards developing F1 hybrid varieties",

abstract = "This report describes advances in the technique of embryogenesis from tulip microspores in culture. High temperature pretreatment (32°C) of bulbs, which contain fully developed inflorescences, had a positive effect on the production of microspore embryos. The pretreatment shortened the time for embryo formation and stimulated the progression from the multicellular microspore stage into the embryo proper. Early unicellular microspores were found to be better starting material for culture than mid unicellular microspores. The technique of microspore embryogenesis was applicable to various tulip cultivars. The improved protocol now allows the production of large numbers of microspore-derived plants, and the further evaluation of doubled haploid plant technology for tulip.",

keywords = "Doubled haploid, Homozygous, Tulipa",

author = "J.B.M. Custers and E. Ennik and W. Eikelboom and J.J.M. Dons and {van Lookeren Campagne}, M.M.",

year = "1997",

doi = "10.17660/ActaHortic.1997.430.38",

language = "Undefined/Unknown",

volume = "430",

pages = "259--266",

journal = "Acta Horticulturae",

issn = "0567-7572",

publisher = "International Society for Horticultural Science",

}

TY - JOUR

T1 - Embryogenesis from isolated microspores of tulip : towards developing F1 hybrid varieties

AU - Custers, J.B.M.

AU - Ennik, E.

AU - Eikelboom, W.

AU - Dons, J.J.M.

AU - van Lookeren Campagne, M.M.

PY - 1997

Y1 - 1997

N2 - This report describes advances in the technique of embryogenesis from tulip microspores in culture. High temperature pretreatment (32°C) of bulbs, which contain fully developed inflorescences, had a positive effect on the production of microspore embryos. The pretreatment shortened the time for embryo formation and stimulated the progression from the multicellular microspore stage into the embryo proper. Early unicellular microspores were found to be better starting material for culture than mid unicellular microspores. The technique of microspore embryogenesis was applicable to various tulip cultivars. The improved protocol now allows the production of large numbers of microspore-derived plants, and the further evaluation of doubled haploid plant technology for tulip.

AB - This report describes advances in the technique of embryogenesis from tulip microspores in culture. High temperature pretreatment (32°C) of bulbs, which contain fully developed inflorescences, had a positive effect on the production of microspore embryos. The pretreatment shortened the time for embryo formation and stimulated the progression from the multicellular microspore stage into the embryo proper. Early unicellular microspores were found to be better starting material for culture than mid unicellular microspores. The technique of microspore embryogenesis was applicable to various tulip cultivars. The improved protocol now allows the production of large numbers of microspore-derived plants, and the further evaluation of doubled haploid plant technology for tulip.

KW - Doubled haploid

KW - Homozygous

KW - Tulipa

U2 - 10.17660/ActaHortic.1997.430.38

DO - 10.17660/ActaHortic.1997.430.38

M3 - Article

VL - 430

SP - 259

EP - 266

JO - Acta Horticulturae

JF - Acta Horticulturae

SN - 0567-7572

ER -