This report describes advances in the technique of embryogenesis from tulip microspores in culture. High temperature pretreatment (32°C) of bulbs, which contain fully developed inflorescences, had a positive effect on the production of microspore embryos. The pretreatment shortened the time for embryo formation and stimulated the progression from the multicellular microspore stage into the embryo proper. Early unicellular microspores were found to be better starting material for culture than mid unicellular microspores. The technique of microspore embryogenesis was applicable to various tulip cultivars. The improved protocol now allows the production of large numbers of microspore-derived plants, and the further evaluation of doubled haploid plant technology for tulip.
Custers, J. B. M., Ennik, E., Eikelboom, W., Dons, J. J. M., & van Lookeren Campagne, M. M. (1997). Embryogenesis from isolated microspores of tulip : towards developing F1 hybrid varieties. Acta Horticulturae, 430, 259-266. https://doi.org/10.17660/ActaHortic.1997.430.38