@inbook{b8e22876a10b4267a7d57de031feba30,
title = "Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes",
abstract = "The Electrophoretic Mobility Shift Assay is a straightforward and inexpensive method for the determination and quantification of protein-nucleic acid interactions. It relies on the different mobility of free and protein-bound nucleic acid in a gel matrix during electrophoresis. Nucleic acid affinities of crRNA-Cas complexes can be quantified by calculating the dissociation constant (Kd). Here, we describe how two types of EMSA assays are performed using the Cascade ribonucleoprotein complex from Escherichia coli as an example",
keywords = "Affi nity, Binding assay, Cascade, CRISPR, Electrophoretic mobility shift assay, EMSA, Gel shift, Protein–DNA interaction, RNA guide",
author = "T.A. K{\"u}nne and E.R. Westra and S.J.J. Brouns",
year = "2015",
doi = "10.1007/978-1-4939-2687-9_11",
language = "English",
isbn = "9781493926862",
series = "Methods Molecular Biology",
publisher = "Springer Humana Press",
number = "1311",
pages = "171--184",
editor = "M. Lundgren and E. Charpentier and P.C. Fineran",
booktitle = "CRISPR : Methods and Protocols",
}