Efficient somatic embryogenesis in Alstroemeria

J.B. Kim, C.J.J.M. Raemakers, E. Jacobsen, R.G.F. Visser

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

In Alstroemeria high frequencies of compact embryogenic callus (CEC) induction (40%) and friable embryogenic callus (FEC) induction (15%) were obtained from nodes with axil tissue cultured first on a Murashige and Skoog (MS) medium supplemented with 10 ¿M thidiazuron and 0.5 ¿M indole-3-butyric acid and after that on a Schenk and Hildebrandt (SH) medium supplemented with 9.1 ¿M 2,4-dichlorophenoxy acetic acid and 2.2 ¿M benzylaminopurine (BA). Both types of callus were maintained on modified MS medium supplemented with 20.8 ¿M picloram. CEC and FEC formed somatic embryos and subsequently plants when transferred to MS medium supplemented with 2.2 ¿M BA. Plants were produced after 12 weeks (CEC) or after 16 weeks (FEC) of culture. Regenerated plants were established in the greenhouse and flowered normally
Original languageEnglish
Pages (from-to)233-238
JournalPlant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
Volume86
Issue number2
DOIs
Publication statusPublished - 2006

Keywords

  • plant-regeneration
  • genetic-transformation
  • callus
  • induction
  • cultures
  • embryos
  • cassava
  • growth

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