Efficient somatic embryogenesis in Alstroemeria

J.B. Kim; C.J.J.M. Raemakers; E. Jacobsen; R.G.F. Visser

doi:10.1007/s11240-006-9110-6

Efficient somatic embryogenesis in Alstroemeria

J.B. Kim, C.J.J.M. Raemakers, E. Jacobsen, R.G.F. Visser

Plant Breeding

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

In Alstroemeria high frequencies of compact embryogenic callus (CEC) induction (40%) and friable embryogenic callus (FEC) induction (15%) were obtained from nodes with axil tissue cultured first on a Murashige and Skoog (MS) medium supplemented with 10 ¿M thidiazuron and 0.5 ¿M indole-3-butyric acid and after that on a Schenk and Hildebrandt (SH) medium supplemented with 9.1 ¿M 2,4-dichlorophenoxy acetic acid and 2.2 ¿M benzylaminopurine (BA). Both types of callus were maintained on modified MS medium supplemented with 20.8 ¿M picloram. CEC and FEC formed somatic embryos and subsequently plants when transferred to MS medium supplemented with 2.2 ¿M BA. Plants were produced after 12 weeks (CEC) or after 16 weeks (FEC) of culture. Regenerated plants were established in the greenhouse and flowered normally

Original language	English
Pages (from-to)	233-238
Journal	Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
Volume	86
Issue number	2
DOIs	https://doi.org/10.1007/s11240-006-9110-6
Publication status	Published - 2006

Keywords

plant-regeneration
genetic-transformation
callus
induction
cultures
embryos
cassava
growth

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10.1007/s11240-006-9110-6

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title = "Efficient somatic embryogenesis in Alstroemeria",

abstract = "In Alstroemeria high frequencies of compact embryogenic callus (CEC) induction (40%) and friable embryogenic callus (FEC) induction (15%) were obtained from nodes with axil tissue cultured first on a Murashige and Skoog (MS) medium supplemented with 10 ¿M thidiazuron and 0.5 ¿M indole-3-butyric acid and after that on a Schenk and Hildebrandt (SH) medium supplemented with 9.1 ¿M 2,4-dichlorophenoxy acetic acid and 2.2 ¿M benzylaminopurine (BA). Both types of callus were maintained on modified MS medium supplemented with 20.8 ¿M picloram. CEC and FEC formed somatic embryos and subsequently plants when transferred to MS medium supplemented with 2.2 ¿M BA. Plants were produced after 12 weeks (CEC) or after 16 weeks (FEC) of culture. Regenerated plants were established in the greenhouse and flowered normally",

keywords = "plant-regeneration, genetic-transformation, callus, induction, cultures, embryos, cassava, growth",

author = "J.B. Kim and C.J.J.M. Raemakers and E. Jacobsen and R.G.F. Visser",

year = "2006",

doi = "10.1007/s11240-006-9110-6",

language = "English",

volume = "86",

pages = "233--238",

journal = "Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants",

issn = "0167-6857",

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TY - JOUR

T1 - Efficient somatic embryogenesis in Alstroemeria

AU - Kim, J.B.

AU - Raemakers, C.J.J.M.

AU - Jacobsen, E.

AU - Visser, R.G.F.

PY - 2006

Y1 - 2006

N2 - In Alstroemeria high frequencies of compact embryogenic callus (CEC) induction (40%) and friable embryogenic callus (FEC) induction (15%) were obtained from nodes with axil tissue cultured first on a Murashige and Skoog (MS) medium supplemented with 10 ¿M thidiazuron and 0.5 ¿M indole-3-butyric acid and after that on a Schenk and Hildebrandt (SH) medium supplemented with 9.1 ¿M 2,4-dichlorophenoxy acetic acid and 2.2 ¿M benzylaminopurine (BA). Both types of callus were maintained on modified MS medium supplemented with 20.8 ¿M picloram. CEC and FEC formed somatic embryos and subsequently plants when transferred to MS medium supplemented with 2.2 ¿M BA. Plants were produced after 12 weeks (CEC) or after 16 weeks (FEC) of culture. Regenerated plants were established in the greenhouse and flowered normally

AB - In Alstroemeria high frequencies of compact embryogenic callus (CEC) induction (40%) and friable embryogenic callus (FEC) induction (15%) were obtained from nodes with axil tissue cultured first on a Murashige and Skoog (MS) medium supplemented with 10 ¿M thidiazuron and 0.5 ¿M indole-3-butyric acid and after that on a Schenk and Hildebrandt (SH) medium supplemented with 9.1 ¿M 2,4-dichlorophenoxy acetic acid and 2.2 ¿M benzylaminopurine (BA). Both types of callus were maintained on modified MS medium supplemented with 20.8 ¿M picloram. CEC and FEC formed somatic embryos and subsequently plants when transferred to MS medium supplemented with 2.2 ¿M BA. Plants were produced after 12 weeks (CEC) or after 16 weeks (FEC) of culture. Regenerated plants were established in the greenhouse and flowered normally

KW - plant-regeneration

KW - genetic-transformation

KW - callus

KW - induction

KW - cultures

KW - embryos

KW - cassava

KW - growth

U2 - 10.1007/s11240-006-9110-6

DO - 10.1007/s11240-006-9110-6

M3 - Article

SN - 0167-6857

VL - 86

SP - 233

EP - 238

JO - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants

JF - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants

IS - 2

ER -

Efficient somatic embryogenesis in Alstroemeria

Abstract

Keywords

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