Efficacy of three candidate Rift Valley fever vaccines in sheep

J.A. Kortekaas, A.F.G. Antonis, H.C.M. Kant-Eenbergen, R.P.M. Vloet, A. Vogel-Brink, N.D. Oreshkova, S.M. de Boer, B.J. Bosch, R.J.M. Moormann

Research output: Contribution to journalArticleAcademicpeer-review

49 Citations (Scopus)

Abstract

Rift Valley fever virus (RVFV) is a mosquito-transmitted Bunyavirus that causes high morbidity and mortality among ruminants and humans. The virus is endemic to the African continent and the Arabian Peninsula and continues to spread into new areas. The explosive nature of RVF outbreaks requires that vaccines provide swift protection after a single vaccination. We recently developed several candidate vaccines and here report their efficacy in lambs within three weeks after a single vaccination. The first vaccine comprises the purified ectodomain of the Gn structural glycoprotein formulated in a water-in-oil adjuvant. The second vaccine is based on a Newcastle disease virus-based vector that produces both RVFV structural glycoproteins Gn and Gc. The third vaccine comprises a recently developed nonspreading RVFV. The latter two vaccines were administered without adjuvant. The inactivated whole virus-based vaccine produced by Onderstepoort Biological Products was used as a positive control. Five out of six mock-vaccinated lambs developed high viremia and fever and one lamb succumbed to the challenge infection. A single vaccination with each vaccine resulted in a neutralizing antibody response within three weeks after vaccination and protected lambs from viremia, pyrexia and mortality.
Original languageEnglish
Pages (from-to)3423-3429
JournalVaccine
Volume30
Issue number23
DOIs
Publication statusPublished - 2012

Keywords

  • north-american mosquitos
  • enzootic hepatitis
  • rhesus macaques
  • virus
  • disease
  • protein
  • transcription
  • protection
  • challenge
  • africa

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  • Projects

    RVF Schapen (BO-08-010-023)

    Moormann, R.

    1/01/0931/12/11

    Project: EZproject

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