Efficacy of clarithromycin on biofilm formation of methicillin-resistant Staphylococcus pseudintermedius

Matthew DiCicco, Suresh Neethirajan*, Ameet Singh, J.S. Weese

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)

Abstract

Background: Surgical site infections (SSIs) caused by biofilm-forming methicillin-resistant Staphylococcus pseudintermedius (MRSP) have emerged as the most common hospital-acquired infections in companion animals. No methods currently exist for the therapeutic remediation of SSIs caused by MRSP in biofilms. Clarithromycin (CLA) has been shown to prevent biofilm formation by Staphylococcus aureus. This study aims to assess the in vitro activity of CLA in eradicating MRSP biofilm formation on various materials.Results: Quantitative assay results (P = 0.5126) suggest that CLA does not eradicate MRSP biofilm formation on polystyrene after 4 - 24 h growth periods. Scanning electron micrographs confirmed that CLA did not eradicate MRSP biofilm formed on orthopaedic implants.Conclusions: By determining the in vitro characteristics and activities of MRSP isolates alone and against antibiotics, in vitro models of biofilm related infections can be made. In vitro data suggests that CLA does not effectively eradicate S. pseudintermedius biofilms in therapeutic doses.

Original languageEnglish
Article number225
JournalBMC Veterinary Research
Volume8
DOIs
Publication statusPublished - 21 Nov 2012
Externally publishedYes

Keywords

  • Bacteria
  • Biofilm
  • Macrolide, Clarithromycin
  • Methicillin-resistant
  • MRSP
  • S. pseudintermedius
  • Therapeutic

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