Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemcally infected swine herd

P. Jirawattanapong, N. Stockhofe-Zurwieden, L.A.M.G. van Leengoed, G.P. Binnendijk, H.J. Wisselink, R. Raymakers, T. Cruijsen, C.M.C. van der Peet-Schwering, A. van Nes, M. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

Objective: To evaluate lung lesions at slaughter after three-dose vaccination with a subunit Actinobacillus pleuropneumoniae vaccine containing ApxI, ApxII, ApxIII, and an outer membrane protein. Materials and methods: A total of 430 newborn piglets in a herd endemically infected with A pleuropneumoniae were assigned to control and treatment groups by block randomization at litter level. Pigs vaccinated at 6, 10, and 14 weeks of age and unvaccinated controls were housed in three rooms containing 12 pens each, with treated and control groups mixed in pens. Individual pig data included average daily gain (ADG), number of antimicrobial treatments, and clinical disease. Sera of vaccinated (n = 5) and control pigs (n = 6) collected at 6, 10, 14, 18, and 23 weeks of age were tested for A pleuropneumoniae antibodies using the complement fixation test and ELISAs for Apx toxins and outer membrane protein. At slaughter, lungs were examined for gross and microscopic lesions and cultured for A pleuropneumoniae. Differences in ADG and occurrence of A pleuropneumoniae-related lesions were compared between treatment groups using multilevel mixed models with room and pen as random effects. Results: Actinobacillus pleuropneumoniae-related lesions and ADG did not differ between control and treatment groups. Maternal immunity against A pleuropneumoniae was detected until 10 weeks of age. Moderate vaccine titres were observed after the third vaccination. Implications: Maternal antibody may interfere with the response to subunit A pleuropneumoniae vaccines. Postponing vaccination until 10 to 14 weeks of age might be advisable in herds with high maternal immunity.
LanguageEnglish
Pages193-199
JournalJournal of Swine Health and Production
Volume16
Issue number4
Publication statusPublished - 2008

Fingerprint

Pleuropneumonia
Actinobacillus pleuropneumoniae
Subunit Vaccines
subunit vaccines
Swine
herds
lesions (animal)
swine
maternal immunity
average daily gain
Vaccination
Vaccines
outer membrane proteins
vaccination
Mothers
vaccines
Control Groups
Immunity
slaughter
Membrane Proteins

Keywords

  • endobronchial challenge
  • apx toxins
  • pigs
  • protection
  • antibodies
  • pathogenesis
  • serovar-15
  • serotype-9
  • virulence

Cite this

Jirawattanapong, P., Stockhofe-Zurwieden, N., van Leengoed, L. A. M. G., Binnendijk, G. P., Wisselink, H. J., Raymakers, R., ... Nielen, M. (2008). Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemcally infected swine herd. Journal of Swine Health and Production, 16(4), 193-199.
Jirawattanapong, P. ; Stockhofe-Zurwieden, N. ; van Leengoed, L.A.M.G. ; Binnendijk, G.P. ; Wisselink, H.J. ; Raymakers, R. ; Cruijsen, T. ; van der Peet-Schwering, C.M.C. ; van Nes, A. ; Nielen, M. / Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemcally infected swine herd. In: Journal of Swine Health and Production. 2008 ; Vol. 16, No. 4. pp. 193-199.
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title = "Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemcally infected swine herd",
abstract = "Objective: To evaluate lung lesions at slaughter after three-dose vaccination with a subunit Actinobacillus pleuropneumoniae vaccine containing ApxI, ApxII, ApxIII, and an outer membrane protein. Materials and methods: A total of 430 newborn piglets in a herd endemically infected with A pleuropneumoniae were assigned to control and treatment groups by block randomization at litter level. Pigs vaccinated at 6, 10, and 14 weeks of age and unvaccinated controls were housed in three rooms containing 12 pens each, with treated and control groups mixed in pens. Individual pig data included average daily gain (ADG), number of antimicrobial treatments, and clinical disease. Sera of vaccinated (n = 5) and control pigs (n = 6) collected at 6, 10, 14, 18, and 23 weeks of age were tested for A pleuropneumoniae antibodies using the complement fixation test and ELISAs for Apx toxins and outer membrane protein. At slaughter, lungs were examined for gross and microscopic lesions and cultured for A pleuropneumoniae. Differences in ADG and occurrence of A pleuropneumoniae-related lesions were compared between treatment groups using multilevel mixed models with room and pen as random effects. Results: Actinobacillus pleuropneumoniae-related lesions and ADG did not differ between control and treatment groups. Maternal immunity against A pleuropneumoniae was detected until 10 weeks of age. Moderate vaccine titres were observed after the third vaccination. Implications: Maternal antibody may interfere with the response to subunit A pleuropneumoniae vaccines. Postponing vaccination until 10 to 14 weeks of age might be advisable in herds with high maternal immunity.",
keywords = "endobronchial challenge, apx toxins, pigs, protection, antibodies, pathogenesis, serovar-15, serotype-9, virulence",
author = "P. Jirawattanapong and N. Stockhofe-Zurwieden and {van Leengoed}, L.A.M.G. and G.P. Binnendijk and H.J. Wisselink and R. Raymakers and T. Cruijsen and {van der Peet-Schwering}, C.M.C. and {van Nes}, A. and M. Nielen",
year = "2008",
language = "English",
volume = "16",
pages = "193--199",
journal = "Journal of Swine Health and Production",
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Jirawattanapong, P, Stockhofe-Zurwieden, N, van Leengoed, LAMG, Binnendijk, GP, Wisselink, HJ, Raymakers, R, Cruijsen, T, van der Peet-Schwering, CMC, van Nes, A & Nielen, M 2008, 'Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemcally infected swine herd', Journal of Swine Health and Production, vol. 16, no. 4, pp. 193-199.

Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemcally infected swine herd. / Jirawattanapong, P.; Stockhofe-Zurwieden, N.; van Leengoed, L.A.M.G.; Binnendijk, G.P.; Wisselink, H.J.; Raymakers, R.; Cruijsen, T.; van der Peet-Schwering, C.M.C.; van Nes, A.; Nielen, M.

In: Journal of Swine Health and Production, Vol. 16, No. 4, 2008, p. 193-199.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Efficacy of a subunit vaccine against Actinobacillus pleuropneumoniae in an endemcally infected swine herd

AU - Jirawattanapong, P.

AU - Stockhofe-Zurwieden, N.

AU - van Leengoed, L.A.M.G.

AU - Binnendijk, G.P.

AU - Wisselink, H.J.

AU - Raymakers, R.

AU - Cruijsen, T.

AU - van der Peet-Schwering, C.M.C.

AU - van Nes, A.

AU - Nielen, M.

PY - 2008

Y1 - 2008

N2 - Objective: To evaluate lung lesions at slaughter after three-dose vaccination with a subunit Actinobacillus pleuropneumoniae vaccine containing ApxI, ApxII, ApxIII, and an outer membrane protein. Materials and methods: A total of 430 newborn piglets in a herd endemically infected with A pleuropneumoniae were assigned to control and treatment groups by block randomization at litter level. Pigs vaccinated at 6, 10, and 14 weeks of age and unvaccinated controls were housed in three rooms containing 12 pens each, with treated and control groups mixed in pens. Individual pig data included average daily gain (ADG), number of antimicrobial treatments, and clinical disease. Sera of vaccinated (n = 5) and control pigs (n = 6) collected at 6, 10, 14, 18, and 23 weeks of age were tested for A pleuropneumoniae antibodies using the complement fixation test and ELISAs for Apx toxins and outer membrane protein. At slaughter, lungs were examined for gross and microscopic lesions and cultured for A pleuropneumoniae. Differences in ADG and occurrence of A pleuropneumoniae-related lesions were compared between treatment groups using multilevel mixed models with room and pen as random effects. Results: Actinobacillus pleuropneumoniae-related lesions and ADG did not differ between control and treatment groups. Maternal immunity against A pleuropneumoniae was detected until 10 weeks of age. Moderate vaccine titres were observed after the third vaccination. Implications: Maternal antibody may interfere with the response to subunit A pleuropneumoniae vaccines. Postponing vaccination until 10 to 14 weeks of age might be advisable in herds with high maternal immunity.

AB - Objective: To evaluate lung lesions at slaughter after three-dose vaccination with a subunit Actinobacillus pleuropneumoniae vaccine containing ApxI, ApxII, ApxIII, and an outer membrane protein. Materials and methods: A total of 430 newborn piglets in a herd endemically infected with A pleuropneumoniae were assigned to control and treatment groups by block randomization at litter level. Pigs vaccinated at 6, 10, and 14 weeks of age and unvaccinated controls were housed in three rooms containing 12 pens each, with treated and control groups mixed in pens. Individual pig data included average daily gain (ADG), number of antimicrobial treatments, and clinical disease. Sera of vaccinated (n = 5) and control pigs (n = 6) collected at 6, 10, 14, 18, and 23 weeks of age were tested for A pleuropneumoniae antibodies using the complement fixation test and ELISAs for Apx toxins and outer membrane protein. At slaughter, lungs were examined for gross and microscopic lesions and cultured for A pleuropneumoniae. Differences in ADG and occurrence of A pleuropneumoniae-related lesions were compared between treatment groups using multilevel mixed models with room and pen as random effects. Results: Actinobacillus pleuropneumoniae-related lesions and ADG did not differ between control and treatment groups. Maternal immunity against A pleuropneumoniae was detected until 10 weeks of age. Moderate vaccine titres were observed after the third vaccination. Implications: Maternal antibody may interfere with the response to subunit A pleuropneumoniae vaccines. Postponing vaccination until 10 to 14 weeks of age might be advisable in herds with high maternal immunity.

KW - endobronchial challenge

KW - apx toxins

KW - pigs

KW - protection

KW - antibodies

KW - pathogenesis

KW - serovar-15

KW - serotype-9

KW - virulence

M3 - Article

VL - 16

SP - 193

EP - 199

JO - Journal of Swine Health and Production

T2 - Journal of Swine Health and Production

JF - Journal of Swine Health and Production

SN - 1537-209X

IS - 4

ER -