Effects of Sulfur Assimilation in Pseudomonas fluorescens SS101 on Growth, Defense, and Metabolome of Different Brassicaceae

Je-Seung Jeon, D.W. Etalo, N. Carreno Quintero, C.H. de Vos, J.M. Raaijmakers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Genome-wide analysis of plant-growth-promoting Pseudomonas fluorescens strain SS101 (Pf SS101) followed by site-directed mutagenesis previously suggested that sulfur assimilation may play an important role in growth promotion and induced systemic resistance in Arabidopsis. Here, we
investigated the effects of sulfur metabolism in Pf SS101 on growth, defense, and shoot metabolomes of Arabidopsis and the Brassica crop, Broccoli. Root tips of seedlings of Arabidopsis and two Broccoli cultivars were treated with Pf SS101 or with a mutant disrupted in the adenylsulfate reductase cysH,
a key gene in cysteine and methionine biosynthesis. Phenotyping of plants treated with wild-type Pf SS101 or its cysH mutant revealed that sulfur assimilation in Pf SS101 was associated with enhanced growth of Arabidopsis but with a reduction in shoot biomass of two Broccoli cultivars. Untargeted
metabolomics revealed that cysH-mediated sulfur assimilation in Pf SS101 had significant effects on shoot chemistry of Arabidopsis, in particular on chain elongation of aliphatic glucosinolates (GLSs) and on indole metabolites, including camalexin and the growth hormone indole-3-acetic acid. In
Broccoli, Pf SS101 sulfur assimilation significantly upregulated the relative abundance of several shoot metabolites, in particular, indolic GLSs and phenylpropanoids. These metabolome changes in Broccoli plants coincided with Pf SS101-mediated suppression of leaf infections by Xanthomonas campestris.
Our study showed the metabolic interconnectedness of plants and their root-associated microbiota.
Original languageEnglish
Article number1704
JournalBiomolecules
Volume11
Issue number11
DOIs
Publication statusPublished - 16 Nov 2021

Keywords

  • Flavonoids
  • Glucosinolates (GLSs)
  • Induced systemic resistance
  • Plant growth promotion
  • Plant metabolomics
  • Pseudomonas fluorescens

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