Several long-chain polyunsaturated fatty acids can exert an inhibitory effect on the expression of lipogenic genes, including acetyl- CoA carboxylase (ACC), fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD1), in the bovine mammary gland. Nevertheless, the effect of the short-chain fatty acids acetate (Ac) and ß-hydroxybutyrate (BHBA), which are the main precursors for de novo fatty acid synthesis, on expression of these lipogenic genes remains unclear. To examine these effects, we used a bovine mammary epithelial cell line (MAC-T) incubated in fatty acid-free media (CON) and added either 5 mM Ac, 5 mM BHBA or a combination of 5 mM Ac + 5 mM BHBA. Furthermore, MAC-T cells were treated with either 100 µM palmitic acid (PA), 100 µM stearic acid (SA), 100 µM oleic acid (OA), 100 µM trans-vaccenic acid (TVA), 100 µM linoleic acid (LA) or 100 µM a-linolenic acid (ALA), which were complexed with BSA. Cells were grown to confluence in DMEM-F12 with 10% serum and all treatments were performed in triplicate. Fatty acids were added to the cells in serum-free DMEM-F12 including the hormones insulin, prolactin, apo-transferrin, progesterone and hydrocortisone. After 12 h of incubation, total RNA was extracted from the cells and mRNA expression of ACC, FAS and SCD1 was analyzed by real-time PCR. In comparison with CON, expression of ACC was increased by Ac (+44%) and was reduced by LA (-48%) and ALA (-49%). Expression of SCD1 was increased by Ac (+61%) and was reduced by OA (-61%), LA (-84%) and ALA (-88%). The treatments did not significantly affect expression of FAS compared with CON. Our results demonstrate that Ac upregulates the expression of ACC and SCD1 in MAC-T cells, which indicates that Ac may increase de novo synthesis and desaturation of fatty acids in the bovine mammary gland.
|Title of host publication||2011 annual Meeting abstracts|
|Publication status||Published - 2011|
|Event||ADSA/ASAS New Orleans, Louisiana - |
Duration: 10 Jul 2011 → 14 Jul 2011
|Conference||ADSA/ASAS New Orleans, Louisiana|
|Period||10/07/11 → 14/07/11|