Effects of short- and long-chain fatty acids on expression of lipgenic genes in bovine mammary epithelial cells

A.A.A. Jacobs, J.S. Liesman, M.J. van Haaren, J. Dijkstra, A.M. van Vuuren, J. van Baal

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

Several long-chain polyunsaturated fatty acids can exert an inhibitory effect on the expression of lipogenic genes, including acetyl- CoA carboxylase (ACC), fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD1), in the bovine mammary gland. Nevertheless, the effect of the short-chain fatty acids acetate (Ac) and ß-hydroxybutyrate (BHBA), which are the main precursors for de novo fatty acid synthesis, on expression of these lipogenic genes remains unclear. To examine these effects, we used a bovine mammary epithelial cell line (MAC-T) incubated in fatty acid-free media (CON) and added either 5 mM Ac, 5 mM BHBA or a combination of 5 mM Ac + 5 mM BHBA. Furthermore, MAC-T cells were treated with either 100 µM palmitic acid (PA), 100 µM stearic acid (SA), 100 µM oleic acid (OA), 100 µM trans-vaccenic acid (TVA), 100 µM linoleic acid (LA) or 100 µM a-linolenic acid (ALA), which were complexed with BSA. Cells were grown to confluence in DMEM-F12 with 10% serum and all treatments were performed in triplicate. Fatty acids were added to the cells in serum-free DMEM-F12 including the hormones insulin, prolactin, apo-transferrin, progesterone and hydrocortisone. After 12 h of incubation, total RNA was extracted from the cells and mRNA expression of ACC, FAS and SCD1 was analyzed by real-time PCR. In comparison with CON, expression of ACC was increased by Ac (+44%) and was reduced by LA (-48%) and ALA (-49%). Expression of SCD1 was increased by Ac (+61%) and was reduced by OA (-61%), LA (-84%) and ALA (-88%). The treatments did not significantly affect expression of FAS compared with CON. Our results demonstrate that Ac upregulates the expression of ACC and SCD1 in MAC-T cells, which indicates that Ac may increase de novo synthesis and desaturation of fatty acids in the bovine mammary gland.
Original languageEnglish
Title of host publication2011 annual Meeting abstracts
Pages750
Volume94
Publication statusPublished - 2011
EventADSA/ASAS New Orleans, Louisiana -
Duration: 10 Jul 201114 Jul 2011

Conference

ConferenceADSA/ASAS New Orleans, Louisiana
Period10/07/1114/07/11

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