Effects of Salmonella on spatial-temporal processes of jejunal development in chickens

D.J. Schokker, M.A. Smits, A.J.W. Hoekman, H.K. Parmentier, J.M.J. Rebel

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

To study effects of Salmonella enteritidis on morphological and functional changes in chicken jejunal development, we analysed gene expression profiles at seven points post-infection in 1-21 day-old broiler chickens. Nine clusters with different gene expression patterns were identified, and the genes in each cluster were further analyzed by a functional annotation clustering method (DAVID). Functional and morphological developmental processes dominated in all the nine clusters. Salmonella infection caused delays in several intestinal-morphological processes, whereas functional metabolic processes occurred in a similar spatial-temporal frame compared to normal jejunum development. A clear difference between normal developing- and Salmonella disturbed jejunum was the higher expression of genes involved in cell turn-over at early stages in the infected jejunum. Surprisingly, we found no clustered immune related processes in the infected birds. To compare the immunological processes between control and Salmonella infected chickens, the gene expression data was superimposed on known immunological KEGG pathways. Furthermore an in-depth analysis on the immune gene level was performed. As expected, we did find immunological processes in the Salmonella infected jejunum. Several of these processes could be verified by immunohistochemistry measurements of different immunological cell types. However, the well-ordered spatial-temporal development of the immune system, as observed in control non-infected animals, was completely abolished in the infected animals. Several immunological processes started much earlier in time, whereas other processes are disorganised. These data indicate that normal morphological and immunological development of jejunum is changed dramatically by a disturbance due to Salmonella infection. Due to the disturbance, the well-organized spatial-temporal development of morphological processes are delayed, those of the immunological development are scattered, whereas metabolic functional processes are almost not affected. This demonstrates the flexibility of developmental processes in the broiler chicken intestine. (C) 2010 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1090-1100
JournalDevelopmental and Comparative Immunology
Volume34
Issue number10
DOIs
Publication statusPublished - 2010

Fingerprint

Jejunum
Salmonella
Chickens
Salmonella Infections
Gene Expression
Salmonella enteritidis
Transcriptome
Genes
Intestines
Birds
Cluster Analysis
Immune System
Immunohistochemistry
Infection

Keywords

  • coagulation factor-xiii
  • regulatory t-cells
  • gene-expression
  • immune-responses
  • broiler-chickens
  • small-intestine
  • gastrointestinal-tract
  • kyoto encyclopedia
  • barrier function
  • receptor system

Cite this

@article{3011a8a9942641728debb22049f4a068,
title = "Effects of Salmonella on spatial-temporal processes of jejunal development in chickens",
abstract = "To study effects of Salmonella enteritidis on morphological and functional changes in chicken jejunal development, we analysed gene expression profiles at seven points post-infection in 1-21 day-old broiler chickens. Nine clusters with different gene expression patterns were identified, and the genes in each cluster were further analyzed by a functional annotation clustering method (DAVID). Functional and morphological developmental processes dominated in all the nine clusters. Salmonella infection caused delays in several intestinal-morphological processes, whereas functional metabolic processes occurred in a similar spatial-temporal frame compared to normal jejunum development. A clear difference between normal developing- and Salmonella disturbed jejunum was the higher expression of genes involved in cell turn-over at early stages in the infected jejunum. Surprisingly, we found no clustered immune related processes in the infected birds. To compare the immunological processes between control and Salmonella infected chickens, the gene expression data was superimposed on known immunological KEGG pathways. Furthermore an in-depth analysis on the immune gene level was performed. As expected, we did find immunological processes in the Salmonella infected jejunum. Several of these processes could be verified by immunohistochemistry measurements of different immunological cell types. However, the well-ordered spatial-temporal development of the immune system, as observed in control non-infected animals, was completely abolished in the infected animals. Several immunological processes started much earlier in time, whereas other processes are disorganised. These data indicate that normal morphological and immunological development of jejunum is changed dramatically by a disturbance due to Salmonella infection. Due to the disturbance, the well-organized spatial-temporal development of morphological processes are delayed, those of the immunological development are scattered, whereas metabolic functional processes are almost not affected. This demonstrates the flexibility of developmental processes in the broiler chicken intestine. (C) 2010 Elsevier Ltd. All rights reserved.",
keywords = "coagulation factor-xiii, regulatory t-cells, gene-expression, immune-responses, broiler-chickens, small-intestine, gastrointestinal-tract, kyoto encyclopedia, barrier function, receptor system",
author = "D.J. Schokker and M.A. Smits and A.J.W. Hoekman and H.K. Parmentier and J.M.J. Rebel",
year = "2010",
doi = "10.1016/j.dci.2010.05.013",
language = "English",
volume = "34",
pages = "1090--1100",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier",
number = "10",

}

Effects of Salmonella on spatial-temporal processes of jejunal development in chickens. / Schokker, D.J.; Smits, M.A.; Hoekman, A.J.W.; Parmentier, H.K.; Rebel, J.M.J.

In: Developmental and Comparative Immunology, Vol. 34, No. 10, 2010, p. 1090-1100.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effects of Salmonella on spatial-temporal processes of jejunal development in chickens

AU - Schokker, D.J.

AU - Smits, M.A.

AU - Hoekman, A.J.W.

AU - Parmentier, H.K.

AU - Rebel, J.M.J.

PY - 2010

Y1 - 2010

N2 - To study effects of Salmonella enteritidis on morphological and functional changes in chicken jejunal development, we analysed gene expression profiles at seven points post-infection in 1-21 day-old broiler chickens. Nine clusters with different gene expression patterns were identified, and the genes in each cluster were further analyzed by a functional annotation clustering method (DAVID). Functional and morphological developmental processes dominated in all the nine clusters. Salmonella infection caused delays in several intestinal-morphological processes, whereas functional metabolic processes occurred in a similar spatial-temporal frame compared to normal jejunum development. A clear difference between normal developing- and Salmonella disturbed jejunum was the higher expression of genes involved in cell turn-over at early stages in the infected jejunum. Surprisingly, we found no clustered immune related processes in the infected birds. To compare the immunological processes between control and Salmonella infected chickens, the gene expression data was superimposed on known immunological KEGG pathways. Furthermore an in-depth analysis on the immune gene level was performed. As expected, we did find immunological processes in the Salmonella infected jejunum. Several of these processes could be verified by immunohistochemistry measurements of different immunological cell types. However, the well-ordered spatial-temporal development of the immune system, as observed in control non-infected animals, was completely abolished in the infected animals. Several immunological processes started much earlier in time, whereas other processes are disorganised. These data indicate that normal morphological and immunological development of jejunum is changed dramatically by a disturbance due to Salmonella infection. Due to the disturbance, the well-organized spatial-temporal development of morphological processes are delayed, those of the immunological development are scattered, whereas metabolic functional processes are almost not affected. This demonstrates the flexibility of developmental processes in the broiler chicken intestine. (C) 2010 Elsevier Ltd. All rights reserved.

AB - To study effects of Salmonella enteritidis on morphological and functional changes in chicken jejunal development, we analysed gene expression profiles at seven points post-infection in 1-21 day-old broiler chickens. Nine clusters with different gene expression patterns were identified, and the genes in each cluster were further analyzed by a functional annotation clustering method (DAVID). Functional and morphological developmental processes dominated in all the nine clusters. Salmonella infection caused delays in several intestinal-morphological processes, whereas functional metabolic processes occurred in a similar spatial-temporal frame compared to normal jejunum development. A clear difference between normal developing- and Salmonella disturbed jejunum was the higher expression of genes involved in cell turn-over at early stages in the infected jejunum. Surprisingly, we found no clustered immune related processes in the infected birds. To compare the immunological processes between control and Salmonella infected chickens, the gene expression data was superimposed on known immunological KEGG pathways. Furthermore an in-depth analysis on the immune gene level was performed. As expected, we did find immunological processes in the Salmonella infected jejunum. Several of these processes could be verified by immunohistochemistry measurements of different immunological cell types. However, the well-ordered spatial-temporal development of the immune system, as observed in control non-infected animals, was completely abolished in the infected animals. Several immunological processes started much earlier in time, whereas other processes are disorganised. These data indicate that normal morphological and immunological development of jejunum is changed dramatically by a disturbance due to Salmonella infection. Due to the disturbance, the well-organized spatial-temporal development of morphological processes are delayed, those of the immunological development are scattered, whereas metabolic functional processes are almost not affected. This demonstrates the flexibility of developmental processes in the broiler chicken intestine. (C) 2010 Elsevier Ltd. All rights reserved.

KW - coagulation factor-xiii

KW - regulatory t-cells

KW - gene-expression

KW - immune-responses

KW - broiler-chickens

KW - small-intestine

KW - gastrointestinal-tract

KW - kyoto encyclopedia

KW - barrier function

KW - receptor system

U2 - 10.1016/j.dci.2010.05.013

DO - 10.1016/j.dci.2010.05.013

M3 - Article

VL - 34

SP - 1090

EP - 1100

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 10

ER -