Effects of rye inclusion in diets on broiler performance, gut morphology and microbiota composition

M.M. van Krimpen, Mehran Torki, S.M. Borgijink, D. Schokker, S.A. Vastenhouw, F.M. de Bree, A. Bossers, T. Fabri, Naomi de Bruijn, A.J.M. Jansman, J.M.J. Rebel, M.A. Smits, R.A. van Emous

Research output: Contribution to conferenceAbstractAcademic

Abstract

It has been hypothesized that dietary inclusion of rye would increase viscosity of intestinal
digesta, consequently resulting in an effect on nutrient absorption, gut wall morphology,
composition of microbiota, and immunity-related processes in the gut wall, and it might be a
helpful model ingredient to investigate the negative effects of nutrition on immune competence
parameters of the birds. In this experiment the effects of dietary inclusion of three levels (0, 5,
and 10%) of rye between 14 and 28 days of age on gut health, digesta microbiota composition,
expression of genes in the small intestinal tissue and performance in broilers were investigated.
A total of 960 day-old male Ross 308 chicks were allocated to 24 pens (40 birds per pen).
Inclusion of 10% rye in the diet did not affect feed intake, but decreased body weight gain, and
increased feed conversion ratio. Litter quality was inversely related to the level of rye inclusion
in the diet. Providing rye-rich diets resulted in increased jejunal villus height and crypt depth
during the first week of provision, whereas the villus-crypt ratio was not affected. During the
second week of the experiment, however, the level of rye inclusion had no effect on jejunal
gut morphology. Inclusion of rye into the diet did not affect the number and size of jejunal
goblet cells. Dietary inclusion of rye did not affect the diversity of the jejunal microbiota,
as determined by the Shannon index, although specific microbial strains were affected by
rye inclusion. Lactobacillus species made about 75-80% of the jejunal microbiota, and rye
inclusion resulted in an exchange between the different lactobacillus species. At d28, the share
of Lactobacillus reuteri, Staphylococcus saporphyticus and Aerococcaceae in the microbiota in jejunal digesta decreased with increasing dietary rye. Dietary rye inclusion affected expression of genes in the small intestinal tissue involved in cell cycle processes of the epithelial cells, including proliferation, differentiation, motility, and survival, as well as in the complement and coagulation cascade. At 28 d of age, effects were more pronounced in birds fed the 10% rye diet, compared to birds fed the 5% rye diet. In conclusion, inclusion of 5% or 10% rye to the grower diet of broilers have limited effects on performance. Ileal gut morphology, microbiota composition of jejunal digesta, and gene expression profiles of jejunal tissue; however, were affected by dietary rye inclusion level.
Original languageEnglish
Pages65
Number of pages1
Publication statusPublished - 20 Jun 2016
Event16th International Conference on Production Diseases in Farm Animals - Wageningen University (Hof van Wageningen Conference Centre), Wageningen, Netherlands
Duration: 22 Jun 201623 Jun 2016
Conference number: 16
http://www.wageningenur.nl/en/activity/16th-International-Conference-on-Production-Diseases-in-Farm-Animals-.htm

Conference

Conference16th International Conference on Production Diseases in Farm Animals
Abbreviated titleICPD 2016
CountryNetherlands
CityWageningen
Period22/06/1623/06/16
Internet address

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rye
digestive system
broiler chickens
diet
digesta
birds
villi
microbiome
Lactobacillus
gene expression
Lactobacillus reuteri
grower diets
coagulation
Staphylococcus
litters (young animals)
cell cycle
cell proliferation
complement
viscosity
epithelial cells

Cite this

van Krimpen, M. M., Torki, M., Borgijink, S. M., Schokker, D., Vastenhouw, S. A., de Bree, F. M., ... van Emous, R. A. (2016). Effects of rye inclusion in diets on broiler performance, gut morphology and microbiota composition. 65. Abstract from 16th International Conference on Production Diseases in Farm Animals , Wageningen, Netherlands.
van Krimpen, M.M. ; Torki, Mehran ; Borgijink, S.M. ; Schokker, D. ; Vastenhouw, S.A. ; de Bree, F.M. ; Bossers, A. ; Fabri, T. ; de Bruijn, Naomi ; Jansman, A.J.M. ; Rebel, J.M.J. ; Smits, M.A. ; van Emous, R.A. / Effects of rye inclusion in diets on broiler performance, gut morphology and microbiota composition. Abstract from 16th International Conference on Production Diseases in Farm Animals , Wageningen, Netherlands.1 p.
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title = "Effects of rye inclusion in diets on broiler performance, gut morphology and microbiota composition",
abstract = "It has been hypothesized that dietary inclusion of rye would increase viscosity of intestinaldigesta, consequently resulting in an effect on nutrient absorption, gut wall morphology,composition of microbiota, and immunity-related processes in the gut wall, and it might be ahelpful model ingredient to investigate the negative effects of nutrition on immune competenceparameters of the birds. In this experiment the effects of dietary inclusion of three levels (0, 5,and 10{\%}) of rye between 14 and 28 days of age on gut health, digesta microbiota composition,expression of genes in the small intestinal tissue and performance in broilers were investigated.A total of 960 day-old male Ross 308 chicks were allocated to 24 pens (40 birds per pen).Inclusion of 10{\%} rye in the diet did not affect feed intake, but decreased body weight gain, andincreased feed conversion ratio. Litter quality was inversely related to the level of rye inclusionin the diet. Providing rye-rich diets resulted in increased jejunal villus height and crypt depthduring the first week of provision, whereas the villus-crypt ratio was not affected. During thesecond week of the experiment, however, the level of rye inclusion had no effect on jejunalgut morphology. Inclusion of rye into the diet did not affect the number and size of jejunalgoblet cells. Dietary inclusion of rye did not affect the diversity of the jejunal microbiota,as determined by the Shannon index, although specific microbial strains were affected byrye inclusion. Lactobacillus species made about 75-80{\%} of the jejunal microbiota, and ryeinclusion resulted in an exchange between the different lactobacillus species. At d28, the shareof Lactobacillus reuteri, Staphylococcus saporphyticus and Aerococcaceae in the microbiota in jejunal digesta decreased with increasing dietary rye. Dietary rye inclusion affected expression of genes in the small intestinal tissue involved in cell cycle processes of the epithelial cells, including proliferation, differentiation, motility, and survival, as well as in the complement and coagulation cascade. At 28 d of age, effects were more pronounced in birds fed the 10{\%} rye diet, compared to birds fed the 5{\%} rye diet. In conclusion, inclusion of 5{\%} or 10{\%} rye to the grower diet of broilers have limited effects on performance. Ileal gut morphology, microbiota composition of jejunal digesta, and gene expression profiles of jejunal tissue; however, were affected by dietary rye inclusion level.",
author = "{van Krimpen}, M.M. and Mehran Torki and S.M. Borgijink and D. Schokker and S.A. Vastenhouw and {de Bree}, F.M. and A. Bossers and T. Fabri and {de Bruijn}, Naomi and A.J.M. Jansman and J.M.J. Rebel and M.A. Smits and {van Emous}, R.A.",
year = "2016",
month = "6",
day = "20",
language = "English",
pages = "65",
note = "16th International Conference on Production Diseases in Farm Animals , ICPD 2016 ; Conference date: 22-06-2016 Through 23-06-2016",
url = "http://www.wageningenur.nl/en/activity/16th-International-Conference-on-Production-Diseases-in-Farm-Animals-.htm",

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van Krimpen, MM, Torki, M, Borgijink, SM, Schokker, D, Vastenhouw, SA, de Bree, FM, Bossers, A, Fabri, T, de Bruijn, N, Jansman, AJM, Rebel, JMJ, Smits, MA & van Emous, RA 2016, 'Effects of rye inclusion in diets on broiler performance, gut morphology and microbiota composition' 16th International Conference on Production Diseases in Farm Animals , Wageningen, Netherlands, 22/06/16 - 23/06/16, pp. 65.

Effects of rye inclusion in diets on broiler performance, gut morphology and microbiota composition. / van Krimpen, M.M.; Torki, Mehran; Borgijink, S.M.; Schokker, D.; Vastenhouw, S.A.; de Bree, F.M.; Bossers, A.; Fabri, T.; de Bruijn, Naomi; Jansman, A.J.M.; Rebel, J.M.J.; Smits, M.A.; van Emous, R.A.

2016. 65 Abstract from 16th International Conference on Production Diseases in Farm Animals , Wageningen, Netherlands.

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Effects of rye inclusion in diets on broiler performance, gut morphology and microbiota composition

AU - van Krimpen, M.M.

AU - Torki, Mehran

AU - Borgijink, S.M.

AU - Schokker, D.

AU - Vastenhouw, S.A.

AU - de Bree, F.M.

AU - Bossers, A.

AU - Fabri, T.

AU - de Bruijn, Naomi

AU - Jansman, A.J.M.

AU - Rebel, J.M.J.

AU - Smits, M.A.

AU - van Emous, R.A.

PY - 2016/6/20

Y1 - 2016/6/20

N2 - It has been hypothesized that dietary inclusion of rye would increase viscosity of intestinaldigesta, consequently resulting in an effect on nutrient absorption, gut wall morphology,composition of microbiota, and immunity-related processes in the gut wall, and it might be ahelpful model ingredient to investigate the negative effects of nutrition on immune competenceparameters of the birds. In this experiment the effects of dietary inclusion of three levels (0, 5,and 10%) of rye between 14 and 28 days of age on gut health, digesta microbiota composition,expression of genes in the small intestinal tissue and performance in broilers were investigated.A total of 960 day-old male Ross 308 chicks were allocated to 24 pens (40 birds per pen).Inclusion of 10% rye in the diet did not affect feed intake, but decreased body weight gain, andincreased feed conversion ratio. Litter quality was inversely related to the level of rye inclusionin the diet. Providing rye-rich diets resulted in increased jejunal villus height and crypt depthduring the first week of provision, whereas the villus-crypt ratio was not affected. During thesecond week of the experiment, however, the level of rye inclusion had no effect on jejunalgut morphology. Inclusion of rye into the diet did not affect the number and size of jejunalgoblet cells. Dietary inclusion of rye did not affect the diversity of the jejunal microbiota,as determined by the Shannon index, although specific microbial strains were affected byrye inclusion. Lactobacillus species made about 75-80% of the jejunal microbiota, and ryeinclusion resulted in an exchange between the different lactobacillus species. At d28, the shareof Lactobacillus reuteri, Staphylococcus saporphyticus and Aerococcaceae in the microbiota in jejunal digesta decreased with increasing dietary rye. Dietary rye inclusion affected expression of genes in the small intestinal tissue involved in cell cycle processes of the epithelial cells, including proliferation, differentiation, motility, and survival, as well as in the complement and coagulation cascade. At 28 d of age, effects were more pronounced in birds fed the 10% rye diet, compared to birds fed the 5% rye diet. In conclusion, inclusion of 5% or 10% rye to the grower diet of broilers have limited effects on performance. Ileal gut morphology, microbiota composition of jejunal digesta, and gene expression profiles of jejunal tissue; however, were affected by dietary rye inclusion level.

AB - It has been hypothesized that dietary inclusion of rye would increase viscosity of intestinaldigesta, consequently resulting in an effect on nutrient absorption, gut wall morphology,composition of microbiota, and immunity-related processes in the gut wall, and it might be ahelpful model ingredient to investigate the negative effects of nutrition on immune competenceparameters of the birds. In this experiment the effects of dietary inclusion of three levels (0, 5,and 10%) of rye between 14 and 28 days of age on gut health, digesta microbiota composition,expression of genes in the small intestinal tissue and performance in broilers were investigated.A total of 960 day-old male Ross 308 chicks were allocated to 24 pens (40 birds per pen).Inclusion of 10% rye in the diet did not affect feed intake, but decreased body weight gain, andincreased feed conversion ratio. Litter quality was inversely related to the level of rye inclusionin the diet. Providing rye-rich diets resulted in increased jejunal villus height and crypt depthduring the first week of provision, whereas the villus-crypt ratio was not affected. During thesecond week of the experiment, however, the level of rye inclusion had no effect on jejunalgut morphology. Inclusion of rye into the diet did not affect the number and size of jejunalgoblet cells. Dietary inclusion of rye did not affect the diversity of the jejunal microbiota,as determined by the Shannon index, although specific microbial strains were affected byrye inclusion. Lactobacillus species made about 75-80% of the jejunal microbiota, and ryeinclusion resulted in an exchange between the different lactobacillus species. At d28, the shareof Lactobacillus reuteri, Staphylococcus saporphyticus and Aerococcaceae in the microbiota in jejunal digesta decreased with increasing dietary rye. Dietary rye inclusion affected expression of genes in the small intestinal tissue involved in cell cycle processes of the epithelial cells, including proliferation, differentiation, motility, and survival, as well as in the complement and coagulation cascade. At 28 d of age, effects were more pronounced in birds fed the 10% rye diet, compared to birds fed the 5% rye diet. In conclusion, inclusion of 5% or 10% rye to the grower diet of broilers have limited effects on performance. Ileal gut morphology, microbiota composition of jejunal digesta, and gene expression profiles of jejunal tissue; however, were affected by dietary rye inclusion level.

M3 - Abstract

SP - 65

ER -

van Krimpen MM, Torki M, Borgijink SM, Schokker D, Vastenhouw SA, de Bree FM et al. Effects of rye inclusion in diets on broiler performance, gut morphology and microbiota composition. 2016. Abstract from 16th International Conference on Production Diseases in Farm Animals , Wageningen, Netherlands.