TY - JOUR
T1 - Effects of processing technologies combined with cell wall degrading enzymes on in vitro degradability of barley
AU - de Vries, S.
AU - Pustjens, A.M.
AU - Schols, H.A.
AU - Hendriks, W.H.
AU - Gerrits, W.J.J.
PY - 2012
Y1 - 2012
N2 - Effects of processing technologies and cell wall degrading enzymes on in vitro degradation of barley were tested in a 5 × 2 factorial arrangement: 5 technologies (unprocessed, wet-milling, extrusion, autoclaving, and acid-autoclaving), with or without enzymes. Upper gastrointestinal tract digestion (Boisen incubation) and large intestinal fermentation (gas production technique) were simulated in duplicate. All technologies increased digestion of DM (13 to 43% units) and starch (22 to 51% units) during Boisen incubation, compared with the unprocessed control (P <0.01). Wet-milling, extrusion, and acid-autoclaving increased CP digestion by 29 to 33% units (P <0.01). Xylanase and ß-glucanase addition increased digestion of DM (~20% units), starch (~20% units), and CP (~10% units) in unprocessed and autoclaved barley (P <0.01). Wet-milling, extrusion, and acid-autoclaving, reduced the extent (50%) and maximum rate (60 to 75%) of fermentation (P <0.01), which appeared to reflect the reduced amount of starch present in the Boisen residues. In conclusion, wet-milling, extrusion, and acid-autoclaving improved in vitro starch and CP digestion in barley, which is related to the cell wall matrix disruption. Addition of xylanases and ß-glucanases improved in vitro starch and CP digestion only in unprocessed barley or barley poorly affected by processing.
AB - Effects of processing technologies and cell wall degrading enzymes on in vitro degradation of barley were tested in a 5 × 2 factorial arrangement: 5 technologies (unprocessed, wet-milling, extrusion, autoclaving, and acid-autoclaving), with or without enzymes. Upper gastrointestinal tract digestion (Boisen incubation) and large intestinal fermentation (gas production technique) were simulated in duplicate. All technologies increased digestion of DM (13 to 43% units) and starch (22 to 51% units) during Boisen incubation, compared with the unprocessed control (P <0.01). Wet-milling, extrusion, and acid-autoclaving increased CP digestion by 29 to 33% units (P <0.01). Xylanase and ß-glucanase addition increased digestion of DM (~20% units), starch (~20% units), and CP (~10% units) in unprocessed and autoclaved barley (P <0.01). Wet-milling, extrusion, and acid-autoclaving, reduced the extent (50%) and maximum rate (60 to 75%) of fermentation (P <0.01), which appeared to reflect the reduced amount of starch present in the Boisen residues. In conclusion, wet-milling, extrusion, and acid-autoclaving improved in vitro starch and CP digestion in barley, which is related to the cell wall matrix disruption. Addition of xylanases and ß-glucanases improved in vitro starch and CP digestion only in unprocessed barley or barley poorly affected by processing.
KW - kinetics
U2 - 10.2527/jas.51416
DO - 10.2527/jas.51416
M3 - Article
SN - 0021-8812
VL - 90
SP - 331
EP - 333
JO - Journal of Animal Science
JF - Journal of Animal Science
IS - supplement 4
ER -