Effects of Long- and Short-Chain Fatty Acids on the Release of Gastrointestinal Hormones using an ex Vivo Porcine Intestinal Tissue Model

T. Voortman, H.F.J. Hendriks, R.F. Witkamp, H.M. Wortelboer

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

Gastrointestinal (GI) peptide hormones play an important role in short-term regulation of food intake and blood glucose levels. Modulating their release is of potential relevance for weight management and possibly diabetes. As currently available models are hard to extrapolate to the human situation, the use of porcine intestinal tissue, collected from slaughter pigs, was investigated for this purpose. Intestinal tissue disks showed a predicted regional release pattern of GI peptides. Various long-chain fatty acids differentially stimulated release of glucagon-like peptide 1 (GLP-1) (up to 500%) and glucagon-like peptide 2 (GLP-2) (up to 200%) from ileal tissue disks, but effects on peptide YY (PYY) did not reach significance. Short-chain fatty acids had no effects on the release of GLP-1, GLP-2, and PYY in either the ileum or colon. In conclusion, this porcine tissue model shows to be of advantageous use in a tiered approach to study the potential of satiety-inducing compounds to be selected for studies in humans.
Original languageEnglish
Pages (from-to)9035-9042
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number36
DOIs
Publication statusPublished - 2012

Fingerprint

gastrointestinal hormones
Gastrointestinal Hormones
Volatile Fatty Acids
long chain fatty acids
short chain fatty acids
Swine
Glucagon-Like Peptide 2
peptide YY
Tissue
Peptide YY
glucagon-like peptide 1
swine
Glucagon-Like Peptide 1
Appetite Regulation
peptide hormones
Peptide Hormones
weight control
Medical problems
satiety
Ileum

Keywords

  • glucagon-like peptide-1
  • enteroendocrine cell-line
  • food-intake
  • cholecystokinin secretion
  • glucose-homeostasis
  • yy
  • glp-1
  • humans
  • stimulation
  • protein

Cite this

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title = "Effects of Long- and Short-Chain Fatty Acids on the Release of Gastrointestinal Hormones using an ex Vivo Porcine Intestinal Tissue Model",
abstract = "Gastrointestinal (GI) peptide hormones play an important role in short-term regulation of food intake and blood glucose levels. Modulating their release is of potential relevance for weight management and possibly diabetes. As currently available models are hard to extrapolate to the human situation, the use of porcine intestinal tissue, collected from slaughter pigs, was investigated for this purpose. Intestinal tissue disks showed a predicted regional release pattern of GI peptides. Various long-chain fatty acids differentially stimulated release of glucagon-like peptide 1 (GLP-1) (up to 500{\%}) and glucagon-like peptide 2 (GLP-2) (up to 200{\%}) from ileal tissue disks, but effects on peptide YY (PYY) did not reach significance. Short-chain fatty acids had no effects on the release of GLP-1, GLP-2, and PYY in either the ileum or colon. In conclusion, this porcine tissue model shows to be of advantageous use in a tiered approach to study the potential of satiety-inducing compounds to be selected for studies in humans.",
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author = "T. Voortman and H.F.J. Hendriks and R.F. Witkamp and H.M. Wortelboer",
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Effects of Long- and Short-Chain Fatty Acids on the Release of Gastrointestinal Hormones using an ex Vivo Porcine Intestinal Tissue Model. / Voortman, T.; Hendriks, H.F.J.; Witkamp, R.F.; Wortelboer, H.M.

In: Journal of Agricultural and Food Chemistry, Vol. 60, No. 36, 2012, p. 9035-9042.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Hendriks, H.F.J.

AU - Witkamp, R.F.

AU - Wortelboer, H.M.

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N2 - Gastrointestinal (GI) peptide hormones play an important role in short-term regulation of food intake and blood glucose levels. Modulating their release is of potential relevance for weight management and possibly diabetes. As currently available models are hard to extrapolate to the human situation, the use of porcine intestinal tissue, collected from slaughter pigs, was investigated for this purpose. Intestinal tissue disks showed a predicted regional release pattern of GI peptides. Various long-chain fatty acids differentially stimulated release of glucagon-like peptide 1 (GLP-1) (up to 500%) and glucagon-like peptide 2 (GLP-2) (up to 200%) from ileal tissue disks, but effects on peptide YY (PYY) did not reach significance. Short-chain fatty acids had no effects on the release of GLP-1, GLP-2, and PYY in either the ileum or colon. In conclusion, this porcine tissue model shows to be of advantageous use in a tiered approach to study the potential of satiety-inducing compounds to be selected for studies in humans.

AB - Gastrointestinal (GI) peptide hormones play an important role in short-term regulation of food intake and blood glucose levels. Modulating their release is of potential relevance for weight management and possibly diabetes. As currently available models are hard to extrapolate to the human situation, the use of porcine intestinal tissue, collected from slaughter pigs, was investigated for this purpose. Intestinal tissue disks showed a predicted regional release pattern of GI peptides. Various long-chain fatty acids differentially stimulated release of glucagon-like peptide 1 (GLP-1) (up to 500%) and glucagon-like peptide 2 (GLP-2) (up to 200%) from ileal tissue disks, but effects on peptide YY (PYY) did not reach significance. Short-chain fatty acids had no effects on the release of GLP-1, GLP-2, and PYY in either the ileum or colon. In conclusion, this porcine tissue model shows to be of advantageous use in a tiered approach to study the potential of satiety-inducing compounds to be selected for studies in humans.

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