Effects of gut microbiota manipulation on ex vivo lipolysis in human abdominal subcutaneous adipocytes

Johan W.E. Jocken*, Dorien Reijnders, Emanuel E. Canfora, Mark V. Boekschoten, Joghum Plat, Gijs H. Goossens, Ellen E. Blaak

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The intestinal microbiota may contribute to the development of obesity by affecting host lipid metabolism and insulin sensitivity. To investigate the effects of microbiota manipulation on ex vivo basal and β-adrenergically-stimulated lipolysis in human adipocytes, 36 obese men were randomized to amoxicillin (broad-spectrum antibiotic), vancomycin (narrow-spectrum antibiotic) or placebo treatment (7 d, 1500 mg/d). Before and after treatment, ex vivo adipose tissue lipolysis was assessed under basal conditions and during stimulation with the non-selective β-agonist isoprenaline using freshly isolated mature adipocytes. Gene (targeted microarray) and protein expression were analyzed to investigate underlying pathways. Antibiotics treatment did not significantly affect basal and maximal isoprenaline-mediated glycerol release from adipocytes. Adipose tissue β-adrenoceptor expression or post-receptor signalling was also not different between groups. In conclusion, 7 d oral antibiotics treatment has no effect on ex vivo lipolysis in mature adipocytes derived from adipose tissue of obese insulin resistant men.

Original languageEnglish
Pages (from-to)106-112
JournalAdipocyte
Volume7
Issue number2
DOIs
Publication statusPublished - 25 Apr 2018

Keywords

  • Adipose Tissue
  • Fatty acid metabolism
  • Insulin resistance
  • Lipolysis
  • Microbiota
  • Obesity

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