Effects of far-red light on fluorescence induction in infiltrated pea leaves under diminished ¿pH and ¿f components of the proton motive force

A.A. Bulychev, V.A. Osipov, D.N. Matorin, W.J. Vredenberg

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Chlorophyll fluorescence induction curves induced by an actinic pulse of red light follow different kinetics in dark-adapted plant leaves and leaves preilluminated with far-red light. This influence of far-red light was abolished in leaves infiltrated with valinomycin known to eliminate the electrical (¿f) component of the proton-motive force and was strongly enhanced in leaves infiltrated with nigericin that abolishes the ¿pH component. The supposed influence of ionophores on different components of the proton motive force was supported by differential effects of these ionophores on the induction curves of the millisecond component of chlorophyll delayed fluorescence. Comparison of fluorescence induction curves with the kinetics of P700 oxidation in the absence and presence of ionophores suggests that valinomycin facilitates a build-up of a rate-limiting step for electron transport at the site of plastoquinone oxidation, whereas nigericin effectively removes limitations at this site. Far-red light was found to be a particularly effective modulator of electron flows in chloroplasts in the absence of ¿pH backpressure on operation of the electron-transport chain.
Original languageEnglish
Pages (from-to)37-45
JournalJournal of Bioenergetics and Biomembranes
Volume45
Issue number1-2
DOIs
Publication statusPublished - 2013

Keywords

  • photoprotective energy-dissipation
  • electron-transport
  • chlorophyll fluorescence
  • delayed fluorescence
  • photosystem-i
  • photosynthetic control
  • charge separation
  • chloroplasts
  • kinetics
  • activation

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