Effects of environmental and natural estrogens on vitellogenin production in hepatocytes of the brown frog (Rana temporaria)

T. Rouhani Rankouhi; J.T. Sanderson; I. van Holsteijn; P. van Kooten; A.T.C. Bosveld; M. van den Berg

doi:10.1016/j.aquatox.2004.09.009

Effects of environmental and natural estrogens on vitellogenin production in hepatocytes of the brown frog (Rana temporaria)

T. Rouhani Rankouhi, J.T. Sanderson, I. van Holsteijn, P. van Kooten, A.T.C. Bosveld, M. van den Berg

Wageningen Environmental Research

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The objective of this study was to investigate the ability of the natural estrogens and synthetic estrogens as well as the estrogen mimics to induce estrogen-receptor mediated vitellogenesis in primary hepatocytes of the brown frog (Rana temporaria). Based on EC50 values the following order was determined for the potency of the estrogens: 17beta-estradiol (EC50: 19-43 nM) approximate to ethynylestradiol (EC50: 13-80 nM) > estrone (EC50: 218-241 nM) > DES (EC50: 338-3537 nM). Exposure to bisphenol A and methoxychlor concentrations up to 100 muM did not have any effect on in vitro vitellogenesis. (C) 2004 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	97-101
Journal	Aquatic Toxicology
Volume	71
Issue number	1
DOIs	https://doi.org/10.1016/j.aquatox.2004.09.009
Publication status	Published - 2005

Keywords

endocrine disruptors
population declines
ethynyl estradiol
in-vitro
amphibians
chemicals
exposure
assay
model
vivo

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10.1016/j.aquatox.2004.09.009

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title = "Effects of environmental and natural estrogens on vitellogenin production in hepatocytes of the brown frog (Rana temporaria)",

abstract = "The objective of this study was to investigate the ability of the natural estrogens and synthetic estrogens as well as the estrogen mimics to induce estrogen-receptor mediated vitellogenesis in primary hepatocytes of the brown frog (Rana temporaria). Based on EC50 values the following order was determined for the potency of the estrogens: 17beta-estradiol (EC50: 19-43 nM) approximate to ethynylestradiol (EC50: 13-80 nM) > estrone (EC50: 218-241 nM) > DES (EC50: 338-3537 nM). Exposure to bisphenol A and methoxychlor concentrations up to 100 muM did not have any effect on in vitro vitellogenesis. (C) 2004 Elsevier B.V. All rights reserved.",

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journal = "Aquatic Toxicology",

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T1 - Effects of environmental and natural estrogens on vitellogenin production in hepatocytes of the brown frog (Rana temporaria)

AU - Rouhani Rankouhi, T.

AU - Sanderson, J.T.

AU - van Holsteijn, I.

AU - van Kooten, P.

AU - Bosveld, A.T.C.

AU - van den Berg, M.

PY - 2005

Y1 - 2005

N2 - The objective of this study was to investigate the ability of the natural estrogens and synthetic estrogens as well as the estrogen mimics to induce estrogen-receptor mediated vitellogenesis in primary hepatocytes of the brown frog (Rana temporaria). Based on EC50 values the following order was determined for the potency of the estrogens: 17beta-estradiol (EC50: 19-43 nM) approximate to ethynylestradiol (EC50: 13-80 nM) > estrone (EC50: 218-241 nM) > DES (EC50: 338-3537 nM). Exposure to bisphenol A and methoxychlor concentrations up to 100 muM did not have any effect on in vitro vitellogenesis. (C) 2004 Elsevier B.V. All rights reserved.

AB - The objective of this study was to investigate the ability of the natural estrogens and synthetic estrogens as well as the estrogen mimics to induce estrogen-receptor mediated vitellogenesis in primary hepatocytes of the brown frog (Rana temporaria). Based on EC50 values the following order was determined for the potency of the estrogens: 17beta-estradiol (EC50: 19-43 nM) approximate to ethynylestradiol (EC50: 13-80 nM) > estrone (EC50: 218-241 nM) > DES (EC50: 338-3537 nM). Exposure to bisphenol A and methoxychlor concentrations up to 100 muM did not have any effect on in vitro vitellogenesis. (C) 2004 Elsevier B.V. All rights reserved.

KW - endocrine disruptors

KW - population declines

KW - ethynyl estradiol

KW - in-vitro

KW - amphibians

KW - chemicals

KW - exposure

KW - assay

KW - model

KW - vivo

U2 - 10.1016/j.aquatox.2004.09.009

DO - 10.1016/j.aquatox.2004.09.009

M3 - Article

SN - 0166-445X

VL - 71

SP - 97

EP - 101

JO - Aquatic Toxicology

JF - Aquatic Toxicology

IS - 1

ER -

Effects of environmental and natural estrogens on vitellogenin production in hepatocytes of the brown frog (Rana temporaria)

Abstract

Keywords

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