Effects of environmental and natural estrogens on vitellogenin production in hepatocytes of the brown frog (Rana temporaria)

T. Rouhani Rankouhi, J.T. Sanderson, I. van Holsteijn, P. van Kooten, A.T.C. Bosveld, M. van den Berg

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27 Citations (Scopus)

Abstract

The objective of this study was to investigate the ability of the natural estrogens and synthetic estrogens as well as the estrogen mimics to induce estrogen-receptor mediated vitellogenesis in primary hepatocytes of the brown frog (Rana temporaria). Based on EC50 values the following order was determined for the potency of the estrogens: 17beta-estradiol (EC50: 19-43 nM) approximate to ethynylestradiol (EC50: 13-80 nM) > estrone (EC50: 218-241 nM) > DES (EC50: 338-3537 nM). Exposure to bisphenol A and methoxychlor concentrations up to 100 muM did not have any effect on in vitro vitellogenesis. (C) 2004 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)97-101
JournalAquatic Toxicology
Volume71
Issue number1
DOIs
Publication statusPublished - 2005

Keywords

  • endocrine disruptors
  • population declines
  • ethynyl estradiol
  • in-vitro
  • amphibians
  • chemicals
  • exposure
  • assay
  • model
  • vivo

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