Effects of dietary supplementation of Gracilaria sp. extracts on fillet quality, oxidative stress, and immune responses in European seabass (Dicentrarchus labrax)

Maria J. Peixoto, Leonardo Magnoni, José F.M. Gonçalves, Robert H. Twijnstra, Anake Kijjoa, Rui Pereira, Arjan P. Palstra, Rodrigo O.A. Ozório*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

The current study evaluated the effects of two different fractions derived from the methanolic extraction of the red seaweed Gracilaria sp. supplemented in European seabass (Dicentrarchus labrax) diets. With that purpose, three experimental diets were prepared: a basal diet (control), a control supplemented with the methanolic extract (GE, 0.5% diet) and a control supplemented with the insoluble residue of the GE extraction (GR, 4.5%). Seabass with an average initial weight of 16.5 ± 0.6 g were fed the experimental diets for 42 days, and the following parameters were evaluated: growth indicators, digestive enzyme activities, immune and oxidative stress responses, fillet pH and color (L*, a*, and b* values), and skin color. The dietary supplementation of GE or GR had no effect on growth performance, digestive enzyme activities, fillet pH, and color. Skin color was significantly lighter (L*) in fish-fed GE (83.9 ± 1.9) and GR (84.3 ± 2.3) diets when compared with the control group (81.9 ± 3.8). The dietary treatments did not affect the oxidative stress biomarkers. Alternative complement pathway (ACH50) was significantly higher in fish-fed GE diet (168.2 ± 13.4 EU mL−1) than in the control diet (113.1 ± 31.4 EU mL−1). No dietary effect was observed on peroxidase and lysozyme activities. The current study indicates that dietary supplementation of Gracilaria sp. methanolic extracts may have little influence on the innate immune system and skin color in seabass.

Original languageEnglish
Pages (from-to)761-770
JournalJournal of Applied Phycology
Volume31
Issue number1
DOIs
Publication statusPublished - Feb 2019

Fingerprint

fillet quality
Gracilaria
Dicentrarchus labrax
immune response
dietary supplements
stress response
oxidative stress
diet
extracts
skin (animal)
color
digestive enzymes
fillets
skin
fish feeds
experimental diets
enzyme activity
edible seaweed
lysozyme
effect

Keywords

  • Dicentrarchus labrax
  • Dietary seaweed supplementation
  • Fillet quality
  • Fish welfare
  • Gracilaria sp. extract
  • Rhodophyta

Cite this

Peixoto, Maria J. ; Magnoni, Leonardo ; Gonçalves, José F.M. ; Twijnstra, Robert H. ; Kijjoa, Anake ; Pereira, Rui ; Palstra, Arjan P. ; Ozório, Rodrigo O.A. / Effects of dietary supplementation of Gracilaria sp. extracts on fillet quality, oxidative stress, and immune responses in European seabass (Dicentrarchus labrax). In: Journal of Applied Phycology. 2019 ; Vol. 31, No. 1. pp. 761-770.
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title = "Effects of dietary supplementation of Gracilaria sp. extracts on fillet quality, oxidative stress, and immune responses in European seabass (Dicentrarchus labrax)",
abstract = "The current study evaluated the effects of two different fractions derived from the methanolic extraction of the red seaweed Gracilaria sp. supplemented in European seabass (Dicentrarchus labrax) diets. With that purpose, three experimental diets were prepared: a basal diet (control), a control supplemented with the methanolic extract (GE, 0.5{\%} diet) and a control supplemented with the insoluble residue of the GE extraction (GR, 4.5{\%}). Seabass with an average initial weight of 16.5 ± 0.6 g were fed the experimental diets for 42 days, and the following parameters were evaluated: growth indicators, digestive enzyme activities, immune and oxidative stress responses, fillet pH and color (L*, a*, and b* values), and skin color. The dietary supplementation of GE or GR had no effect on growth performance, digestive enzyme activities, fillet pH, and color. Skin color was significantly lighter (L*) in fish-fed GE (83.9 ± 1.9) and GR (84.3 ± 2.3) diets when compared with the control group (81.9 ± 3.8). The dietary treatments did not affect the oxidative stress biomarkers. Alternative complement pathway (ACH50) was significantly higher in fish-fed GE diet (168.2 ± 13.4 EU mL−1) than in the control diet (113.1 ± 31.4 EU mL−1). No dietary effect was observed on peroxidase and lysozyme activities. The current study indicates that dietary supplementation of Gracilaria sp. methanolic extracts may have little influence on the innate immune system and skin color in seabass.",
keywords = "Dicentrarchus labrax, Dietary seaweed supplementation, Fillet quality, Fish welfare, Gracilaria sp. extract, Rhodophyta",
author = "Peixoto, {Maria J.} and Leonardo Magnoni and Gon{\cc}alves, {Jos{\'e} F.M.} and Twijnstra, {Robert H.} and Anake Kijjoa and Rui Pereira and Palstra, {Arjan P.} and Oz{\'o}rio, {Rodrigo O.A.}",
year = "2019",
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language = "English",
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Effects of dietary supplementation of Gracilaria sp. extracts on fillet quality, oxidative stress, and immune responses in European seabass (Dicentrarchus labrax). / Peixoto, Maria J.; Magnoni, Leonardo; Gonçalves, José F.M.; Twijnstra, Robert H.; Kijjoa, Anake; Pereira, Rui; Palstra, Arjan P.; Ozório, Rodrigo O.A.

In: Journal of Applied Phycology, Vol. 31, No. 1, 02.2019, p. 761-770.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effects of dietary supplementation of Gracilaria sp. extracts on fillet quality, oxidative stress, and immune responses in European seabass (Dicentrarchus labrax)

AU - Peixoto, Maria J.

AU - Magnoni, Leonardo

AU - Gonçalves, José F.M.

AU - Twijnstra, Robert H.

AU - Kijjoa, Anake

AU - Pereira, Rui

AU - Palstra, Arjan P.

AU - Ozório, Rodrigo O.A.

PY - 2019/2

Y1 - 2019/2

N2 - The current study evaluated the effects of two different fractions derived from the methanolic extraction of the red seaweed Gracilaria sp. supplemented in European seabass (Dicentrarchus labrax) diets. With that purpose, three experimental diets were prepared: a basal diet (control), a control supplemented with the methanolic extract (GE, 0.5% diet) and a control supplemented with the insoluble residue of the GE extraction (GR, 4.5%). Seabass with an average initial weight of 16.5 ± 0.6 g were fed the experimental diets for 42 days, and the following parameters were evaluated: growth indicators, digestive enzyme activities, immune and oxidative stress responses, fillet pH and color (L*, a*, and b* values), and skin color. The dietary supplementation of GE or GR had no effect on growth performance, digestive enzyme activities, fillet pH, and color. Skin color was significantly lighter (L*) in fish-fed GE (83.9 ± 1.9) and GR (84.3 ± 2.3) diets when compared with the control group (81.9 ± 3.8). The dietary treatments did not affect the oxidative stress biomarkers. Alternative complement pathway (ACH50) was significantly higher in fish-fed GE diet (168.2 ± 13.4 EU mL−1) than in the control diet (113.1 ± 31.4 EU mL−1). No dietary effect was observed on peroxidase and lysozyme activities. The current study indicates that dietary supplementation of Gracilaria sp. methanolic extracts may have little influence on the innate immune system and skin color in seabass.

AB - The current study evaluated the effects of two different fractions derived from the methanolic extraction of the red seaweed Gracilaria sp. supplemented in European seabass (Dicentrarchus labrax) diets. With that purpose, three experimental diets were prepared: a basal diet (control), a control supplemented with the methanolic extract (GE, 0.5% diet) and a control supplemented with the insoluble residue of the GE extraction (GR, 4.5%). Seabass with an average initial weight of 16.5 ± 0.6 g were fed the experimental diets for 42 days, and the following parameters were evaluated: growth indicators, digestive enzyme activities, immune and oxidative stress responses, fillet pH and color (L*, a*, and b* values), and skin color. The dietary supplementation of GE or GR had no effect on growth performance, digestive enzyme activities, fillet pH, and color. Skin color was significantly lighter (L*) in fish-fed GE (83.9 ± 1.9) and GR (84.3 ± 2.3) diets when compared with the control group (81.9 ± 3.8). The dietary treatments did not affect the oxidative stress biomarkers. Alternative complement pathway (ACH50) was significantly higher in fish-fed GE diet (168.2 ± 13.4 EU mL−1) than in the control diet (113.1 ± 31.4 EU mL−1). No dietary effect was observed on peroxidase and lysozyme activities. The current study indicates that dietary supplementation of Gracilaria sp. methanolic extracts may have little influence on the innate immune system and skin color in seabass.

KW - Dicentrarchus labrax

KW - Dietary seaweed supplementation

KW - Fillet quality

KW - Fish welfare

KW - Gracilaria sp. extract

KW - Rhodophyta

U2 - 10.1007/s10811-018-1519-x

DO - 10.1007/s10811-018-1519-x

M3 - Article

VL - 31

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EP - 770

JO - Journal of Applied Phycology

JF - Journal of Applied Phycology

SN - 0921-8971

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ER -