Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 3675-3687 |
| Number of pages | 13 |
| Journal | Journal of Experimental Biology |
| Volume | 208 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - 2005 |
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Keywords
- mutations affecting development
- teleost brachydanio-rerio
- mammalian skeletal-muscle
- growth-factor-i
- acetylcholine-receptors
- neuromuscular activity
- myostatin expression
- myogenin gene
- fast-starts
- fish
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Effects of decreased muscle activity on developing axial musculature in nic b107 mutant zebrafish (Danio rerio). / van der Meulen, T.; Schipper, H.; van Leeuwen, J.L.; Kranenbarg, S.
In: Journal of Experimental Biology, Vol. 208, No. 19, 2005, p. 3675-3687.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Effects of decreased muscle activity on developing axial musculature in nic b107 mutant zebrafish (Danio rerio)
AU - van der Meulen, T.
AU - Schipper, H.
AU - van Leeuwen, J.L.
AU - Kranenbarg, S.
PY - 2005
Y1 - 2005
N2 - The present paper discusses the effects of decreased muscle activity (DMA) on embryonic development in the zebrafish. Wild-type zebrafish embryos become mobile around 18 h post-fertilisation, long before the axial musculature is fully differentiated. As a model for DMA, the nicb107 mutant was used. In nicb107 mutant embryos, muscle fibres are mechanically intact and able to contract, but neuronal signalling is defective and the fibres are not activated, rendering the embryos immobile. Despite the immobility, distinguished slow and fast muscle fibres developed at the correct location in the axial muscles, helical muscle fibre arrangements were detected and sarcomere architecture was generated. However, in nicb107 mutant embryos the notochord is flatter and the cross-sectional body shape more rounded, also affecting muscle fibre orientation. The stacking of sarcomeres and myofibril arrangement show a less regular pattern. Finally, expression levels of several genes were changed. Together, these changes in expression indicate that muscle growth is not impeded and energy metabolism is not changed by the decrease in muscle activity but that the composition of muscle is altered. In addition, skin stiffness is affected. In conclusion, the lack of muscle fibre activity did not prevent the basal muscle components developing but influenced further organisation and differentiation of these components.
AB - The present paper discusses the effects of decreased muscle activity (DMA) on embryonic development in the zebrafish. Wild-type zebrafish embryos become mobile around 18 h post-fertilisation, long before the axial musculature is fully differentiated. As a model for DMA, the nicb107 mutant was used. In nicb107 mutant embryos, muscle fibres are mechanically intact and able to contract, but neuronal signalling is defective and the fibres are not activated, rendering the embryos immobile. Despite the immobility, distinguished slow and fast muscle fibres developed at the correct location in the axial muscles, helical muscle fibre arrangements were detected and sarcomere architecture was generated. However, in nicb107 mutant embryos the notochord is flatter and the cross-sectional body shape more rounded, also affecting muscle fibre orientation. The stacking of sarcomeres and myofibril arrangement show a less regular pattern. Finally, expression levels of several genes were changed. Together, these changes in expression indicate that muscle growth is not impeded and energy metabolism is not changed by the decrease in muscle activity but that the composition of muscle is altered. In addition, skin stiffness is affected. In conclusion, the lack of muscle fibre activity did not prevent the basal muscle components developing but influenced further organisation and differentiation of these components.
KW - mutations affecting development
KW - teleost brachydanio-rerio
KW - mammalian skeletal-muscle
KW - growth-factor-i
KW - acetylcholine-receptors
KW - neuromuscular activity
KW - myostatin expression
KW - myogenin gene
KW - fast-starts
KW - fish
U2 - 10.1242/jeb.01826
DO - 10.1242/jeb.01826
M3 - Article
VL - 208
SP - 3675
EP - 3687
JO - Journal of Experimental Biology
JF - Journal of Experimental Biology
SN - 0022-0949
IS - 19
ER -