Effects of alpha-tocopherol on superoxide production and plasma intercellular adhesion molecule-1 and antibodies to oxidized LDL in chronic smokers

L.J. van Tits, F. de Waart, H.L.M. Hak-Lemmers, P. Heijst, J. de Graaf, P.N. Demacker, A.F. Stalenhoef

Research output: Contribution to journalArticleAcademicpeer-review

36 Citations (Scopus)

Abstract

Antioxidants have been postulated to exert beneficial effects in atherosclerosis. Atherosclerosis is associated with raised plasma levels of soluble intercellular adhesion molecule-1 (sICAM-1) and autoantibodies against oxidized low-density lipoprotein (oxLDL). It is not known whether antioxidants affect these plasma factors in chronic smokers. In a randomized double-blind placebo-controlled study involving 128 male normolipidemic chronic smokers the effect of a 2-year a-tocopherol treatment (400 IU dL-a-tocopherol daily) on plasma levels of sICAM-1 and autoantibodies against oxLDL was evaluated. In addition, we monitored production of superoxide by leukocytes ex vivo. It was found that compared to nonsmokers (n = 33) plasma levels of IgG but not IgM autoantibodies against oxLDL and concentrations of sICAM-1 in smokers were significantly elevated (30 and 42%, respectively). After supplementation with a-tocopherol concentration of TBARS in plasma and in vitro oxidizability of LDL had decreased, but autoantibodies and sICAM-1 had not changed. Production of superoxide was not different between a-tocopherol- and placebo-treated smokers. It is concluded that in chronic smokers, long-term treatment with a-tocopherol does not normalize the raised levels of sICAM-1 and autoantibodies against oxLDL, both risk factors for initiation or progression of cardiovascular disease, despite a decrease in in vitro oxidizability of LDL.
Original languageEnglish
Pages (from-to)1122-1129
JournalFree Radical Biology and Medicine
Volume30
Issue number10
DOIs
Publication statusPublished - 2001

Keywords

  • low-density-lipoprotein
  • coronary-artery disease
  • human endothelial-cells
  • apparently healthy-men
  • protein-kinase-c
  • cigarette-smoking
  • vitamin-e
  • carotid atherosclerosis
  • lipid-peroxidation
  • heart-disease

Fingerprint Dive into the research topics of 'Effects of alpha-tocopherol on superoxide production and plasma intercellular adhesion molecule-1 and antibodies to oxidized LDL in chronic smokers'. Together they form a unique fingerprint.

Cite this