Effects and detection of Nandrosol and ractopamine administration in veal calves

Sara Divari*, Enrica Berio, Paola Pregel, Alessandra Sereno, Luca Chiesa, Radmila Pavlovic, Sara Panseri, Toine F.H. Bovee, Bartolomeo Biolatti, Francesca T. Cannizzo

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

The present study describes different effects of the selective androgen receptor modulator (SARM) nandrolone phenylpropionate (Nandrosol) and the β-agonist ractopamine administration in veal calves, and it investigates different strategies applied to trace these molecules. Morphological changes of gonads and accessory glands attributed to androgen effects, such as testicular atrophy, seminiferous tubule diameter reduction and hyperplasia of prostate epithelium, were detected, although SARMs are not described to cause these lesions. The gene expression analysis showed an anabolic activity of Nandrosol in Longissimus dorsi muscle, where myosin heavy chain (MYH) was significantly up-regulated. An IGF1 increase was weakly significant only in Vastus lateralis muscle. In conclusion, the anatomo-histopathological observations and the MYH mRNA up-regulation in Longissimus dorsi muscle confirm the androgenic treatment in experimental animals. The biosensor assay was not enough sensitive to detect residues in urines and only the direct chemical analysis of urine samples confirmed both β-agonist and SARM treatment.

Original languageEnglish
Pages (from-to)706-713
JournalFood Chemistry
Volume221
DOIs
Publication statusPublished - 2017

Keywords

  • Bioassay
  • Gene expression
  • Histopathology
  • LC-MS/MS
  • Nandrosol
  • Ractopamine
  • SARM
  • Veal calves

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    Divari, S., Berio, E., Pregel, P., Sereno, A., Chiesa, L., Pavlovic, R., ... Cannizzo, F. T. (2017). Effects and detection of Nandrosol and ractopamine administration in veal calves. Food Chemistry, 221, 706-713. https://doi.org/10.1016/j.foodchem.2016.11.116