@inbook{3999365136db4402825d17e1dafecd68,
title = "Effectoromics-based identification of cell surface receptors in potato",
abstract = "In modern resistance breeding, effectors have emerged as tools for accelerating and improving the identification of immune receptors. Effector-assisted breeding was pioneered for identifying resistance genes (R genes) against Phytophthora infestans in potato (Solanum tuberosum). Here we show that effectoromics approaches are also well suitable for identifying pathogen recognition receptors (PRRs) that recognize apoplastic effectors. To detect genotypes that recognize apoplastic proteins of P. infestans, routine agroinfiltration and potato virus X (PVX) agroinfection methods can be applied. In addition, protein infiltrations are feasible for assessing responses to apoplastic effectors and aid in confirming results obtained from the aforementioned methods. Protocols for the effectoromics pipeline are provided, starting from phenotyping for effector responses, up to genotyping and PRR gene identification.",
keywords = "Agroinfiltration, Apoplastic effector, Effectoromics, Genetic mapping, Pattern recognition receptors (PRRs), Protein infiltration, PVX agroinfection, Solanum, Yeast protein production",
author = "Emmanouil Domazakis and Xiao Lin and Carolina Aguilera-Galvez and Doret Wouters and Gerard Bijsterbosch and Wolters, {Pieter J.} and Vleeshouwers, {Vivianne G.A.A.}",
year = "2017",
doi = "10.1007/978-1-4939-6859-6_29",
language = "English",
volume = "1578",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "337--353",
editor = "Libo Shan and Ping He",
booktitle = "Plant Pattern Recognition Receptors",
address = "United States",
}