Effectoromics-based identification of cell surface receptors in potato

Emmanouil Domazakis, Xiao Lin, Carolina Aguilera-Galvez, Doret Wouters, Gerard Bijsterbosch, Pieter J. Wolters, Vivianne G.A.A. Vleeshouwers*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

11 Citations (Scopus)


In modern resistance breeding, effectors have emerged as tools for accelerating and improving the identification of immune receptors. Effector-assisted breeding was pioneered for identifying resistance genes (R genes) against Phytophthora infestans in potato (Solanum tuberosum). Here we show that effectoromics approaches are also well suitable for identifying pathogen recognition receptors (PRRs) that recognize apoplastic effectors. To detect genotypes that recognize apoplastic proteins of P. infestans, routine agroinfiltration and potato virus X (PVX) agroinfection methods can be applied. In addition, protein infiltrations are feasible for assessing responses to apoplastic effectors and aid in confirming results obtained from the aforementioned methods. Protocols for the effectoromics pipeline are provided, starting from phenotyping for effector responses, up to genotyping and PRR gene identification.

Original languageEnglish
Title of host publicationPlant Pattern Recognition Receptors
Subtitle of host publicationMethods and Protocols
EditorsLibo Shan, Ping He
PublisherHumana Press Inc.
ISBN (Electronic)9781493968596
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Agroinfiltration
  • Apoplastic effector
  • Effectoromics
  • Genetic mapping
  • Pattern recognition receptors (PRRs)
  • Protein infiltration
  • PVX agroinfection
  • Solanum
  • Yeast protein production

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