Effectoromics-based identification of cell surface receptors in potato

Emmanouil Domazakis, Xiao Lin, Carolina Aguilera-Galvez, Doret Wouters, Gerard Bijsterbosch, Pieter J. Wolters, Vivianne G.A.A. Vleeshouwers*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

In modern resistance breeding, effectors have emerged as tools for accelerating and improving the identification of immune receptors. Effector-assisted breeding was pioneered for identifying resistance genes (R genes) against Phytophthora infestans in potato (Solanum tuberosum). Here we show that effectoromics approaches are also well suitable for identifying pathogen recognition receptors (PRRs) that recognize apoplastic effectors. To detect genotypes that recognize apoplastic proteins of P. infestans, routine agroinfiltration and potato virus X (PVX) agroinfection methods can be applied. In addition, protein infiltrations are feasible for assessing responses to apoplastic effectors and aid in confirming results obtained from the aforementioned methods. Protocols for the effectoromics pipeline are provided, starting from phenotyping for effector responses, up to genotyping and PRR gene identification.

Original languageEnglish
Title of host publicationPlant Pattern Recognition Receptors
Subtitle of host publicationMethods and Protocols
EditorsLibo Shan, Ping He
PublisherHumana Press Inc.
Pages337-353
Volume1578
ISBN (Electronic)9781493968596
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1578
ISSN (Print)1064-3745

Keywords

  • Agroinfiltration
  • Apoplastic effector
  • Effectoromics
  • Genetic mapping
  • Pattern recognition receptors (PRRs)
  • Protein infiltration
  • PVX agroinfection
  • Solanum
  • Yeast protein production

Fingerprint Dive into the research topics of 'Effectoromics-based identification of cell surface receptors in potato'. Together they form a unique fingerprint.

  • Cite this

    Domazakis, E., Lin, X., Aguilera-Galvez, C., Wouters, D., Bijsterbosch, G., Wolters, P. J., & Vleeshouwers, V. G. A. A. (2017). Effectoromics-based identification of cell surface receptors in potato. In L. Shan, & P. He (Eds.), Plant Pattern Recognition Receptors: Methods and Protocols (Vol. 1578, pp. 337-353). (Methods in Molecular Biology; Vol. 1578). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6859-6_29