Effectoromics-based identification of cell surface receptors in potato

Emmanouil Domazakis, Xiao Lin, Carolina Aguilera-Galvez, Doret Wouters, Gerard Bijsterbosch, Pieter J. Wolters, Vivianne G.A.A. Vleeshouwers*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

2 Citations (Scopus)

Abstract

In modern resistance breeding, effectors have emerged as tools for accelerating and improving the identification of immune receptors. Effector-assisted breeding was pioneered for identifying resistance genes (R genes) against Phytophthora infestans in potato (Solanum tuberosum). Here we show that effectoromics approaches are also well suitable for identifying pathogen recognition receptors (PRRs) that recognize apoplastic effectors. To detect genotypes that recognize apoplastic proteins of P. infestans, routine agroinfiltration and potato virus X (PVX) agroinfection methods can be applied. In addition, protein infiltrations are feasible for assessing responses to apoplastic effectors and aid in confirming results obtained from the aforementioned methods. Protocols for the effectoromics pipeline are provided, starting from phenotyping for effector responses, up to genotyping and PRR gene identification.

Original languageEnglish
Title of host publicationPlant Pattern Recognition Receptors
Subtitle of host publicationMethods and Protocols
EditorsLibo Shan, Ping He
PublisherHumana Press Inc.
Pages337-353
Volume1578
ISBN (Electronic)9781493968596
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1578
ISSN (Print)1064-3745

Fingerprint

Phytophthora infestans
Cell Surface Receptors
Solanum tuberosum
Breeding
Potexvirus
Genes
Proteins
Genotype

Keywords

  • Agroinfiltration
  • Apoplastic effector
  • Effectoromics
  • Genetic mapping
  • Pattern recognition receptors (PRRs)
  • Protein infiltration
  • PVX agroinfection
  • Solanum
  • Yeast protein production

Cite this

Domazakis, E., Lin, X., Aguilera-Galvez, C., Wouters, D., Bijsterbosch, G., Wolters, P. J., & Vleeshouwers, V. G. A. A. (2017). Effectoromics-based identification of cell surface receptors in potato. In L. Shan, & P. He (Eds.), Plant Pattern Recognition Receptors: Methods and Protocols (Vol. 1578, pp. 337-353). (Methods in Molecular Biology; Vol. 1578). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6859-6_29
Domazakis, Emmanouil ; Lin, Xiao ; Aguilera-Galvez, Carolina ; Wouters, Doret ; Bijsterbosch, Gerard ; Wolters, Pieter J. ; Vleeshouwers, Vivianne G.A.A. / Effectoromics-based identification of cell surface receptors in potato. Plant Pattern Recognition Receptors: Methods and Protocols. editor / Libo Shan ; Ping He. Vol. 1578 Humana Press Inc., 2017. pp. 337-353 (Methods in Molecular Biology).
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abstract = "In modern resistance breeding, effectors have emerged as tools for accelerating and improving the identification of immune receptors. Effector-assisted breeding was pioneered for identifying resistance genes (R genes) against Phytophthora infestans in potato (Solanum tuberosum). Here we show that effectoromics approaches are also well suitable for identifying pathogen recognition receptors (PRRs) that recognize apoplastic effectors. To detect genotypes that recognize apoplastic proteins of P. infestans, routine agroinfiltration and potato virus X (PVX) agroinfection methods can be applied. In addition, protein infiltrations are feasible for assessing responses to apoplastic effectors and aid in confirming results obtained from the aforementioned methods. Protocols for the effectoromics pipeline are provided, starting from phenotyping for effector responses, up to genotyping and PRR gene identification.",
keywords = "Agroinfiltration, Apoplastic effector, Effectoromics, Genetic mapping, Pattern recognition receptors (PRRs), Protein infiltration, PVX agroinfection, Solanum, Yeast protein production",
author = "Emmanouil Domazakis and Xiao Lin and Carolina Aguilera-Galvez and Doret Wouters and Gerard Bijsterbosch and Wolters, {Pieter J.} and Vleeshouwers, {Vivianne G.A.A.}",
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Domazakis, E, Lin, X, Aguilera-Galvez, C, Wouters, D, Bijsterbosch, G, Wolters, PJ & Vleeshouwers, VGAA 2017, Effectoromics-based identification of cell surface receptors in potato. in L Shan & P He (eds), Plant Pattern Recognition Receptors: Methods and Protocols. vol. 1578, Methods in Molecular Biology, vol. 1578, Humana Press Inc., pp. 337-353. https://doi.org/10.1007/978-1-4939-6859-6_29

Effectoromics-based identification of cell surface receptors in potato. / Domazakis, Emmanouil; Lin, Xiao; Aguilera-Galvez, Carolina; Wouters, Doret; Bijsterbosch, Gerard; Wolters, Pieter J.; Vleeshouwers, Vivianne G.A.A.

Plant Pattern Recognition Receptors: Methods and Protocols. ed. / Libo Shan; Ping He. Vol. 1578 Humana Press Inc., 2017. p. 337-353 (Methods in Molecular Biology; Vol. 1578).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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AU - Wolters, Pieter J.

AU - Vleeshouwers, Vivianne G.A.A.

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AB - In modern resistance breeding, effectors have emerged as tools for accelerating and improving the identification of immune receptors. Effector-assisted breeding was pioneered for identifying resistance genes (R genes) against Phytophthora infestans in potato (Solanum tuberosum). Here we show that effectoromics approaches are also well suitable for identifying pathogen recognition receptors (PRRs) that recognize apoplastic effectors. To detect genotypes that recognize apoplastic proteins of P. infestans, routine agroinfiltration and potato virus X (PVX) agroinfection methods can be applied. In addition, protein infiltrations are feasible for assessing responses to apoplastic effectors and aid in confirming results obtained from the aforementioned methods. Protocols for the effectoromics pipeline are provided, starting from phenotyping for effector responses, up to genotyping and PRR gene identification.

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KW - PVX agroinfection

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KW - Yeast protein production

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Domazakis E, Lin X, Aguilera-Galvez C, Wouters D, Bijsterbosch G, Wolters PJ et al. Effectoromics-based identification of cell surface receptors in potato. In Shan L, He P, editors, Plant Pattern Recognition Receptors: Methods and Protocols. Vol. 1578. Humana Press Inc. 2017. p. 337-353. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6859-6_29