Effect of theobromine consumption on serum lipoprotein profiles in apparently healthy humans with low HDL-cholesterol concentrations

Doris M. Jacobs*, Lotte Smolders, Yuguang Lin, Niels de Roo, Elke A. Trautwein, John van Duynhoven, Ronald P. Mensink, Jogchum Plat, Velitchka V. Mihaleva

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Scope: Theobromine is a major active compound in cocoa with allegedly beneficial effect on high-density-lipoprotein-cholesterol (HDL-CH). We have investigated the effect of theobromine (TB) consumption on the concentrations of triglyceride (TG) and cholesterol (CH) in various lipoprotein (LP) subclasses. Methods: In a randomized, double-blind, placebo-controlled, cross-over study, 44 apparently healthy women and men (age: 60 ± 6 years, BMI: 29 ± 3 kg/m2) with low baseline HDL-CH concentrations consumed a drink supplemented with 500 mg/d theobromine for 4 weeks. TG and CH concentrations in 15 LP subclasses were predicted from diffusion-edited 1H NMR spectra of fasting serum. Results: The LP phenotype of the subjects was characterized by low CH concentrations in the large HDL particles and high TG concentrations in large VLDL and chylomicron (CM) particles, which clearly differed from a LP phenotype of subjects with normal HDL-CH. TB only reduced CH concentrations in the LDL particles by 3.64 and 6.79%, but had no effect on TG and CH in any of the HDL, VLDL and CM subclasses. Conclusion: TB was not effective on HDL-CH in subjects with a LP phenotype characterized by low HDL-CH and high TG in VLDL.
Original languageEnglish
Article number59
JournalFrontiers in Molecular Biosciences
Volume4
Issue numberAUG
DOIs
Publication statusPublished - 24 Aug 2017

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Theobromine
HDL Cholesterol
Lipoproteins
Triglycerides
Cholesterol
Chylomicrons
Serum
Phenotype
Cocoa
LDL Cholesterol
Cross-Over Studies
Fasting
Placebos
Nuclear magnetic resonance

Keywords

  • HDL
  • Lipoprotein
  • NMR
  • PLS model
  • Theobromine

Cite this

Jacobs, Doris M. ; Smolders, Lotte ; Lin, Yuguang ; de Roo, Niels ; Trautwein, Elke A. ; van Duynhoven, John ; Mensink, Ronald P. ; Plat, Jogchum ; Mihaleva, Velitchka V. / Effect of theobromine consumption on serum lipoprotein profiles in apparently healthy humans with low HDL-cholesterol concentrations. In: Frontiers in Molecular Biosciences. 2017 ; Vol. 4, No. AUG.
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title = "Effect of theobromine consumption on serum lipoprotein profiles in apparently healthy humans with low HDL-cholesterol concentrations",
abstract = "Scope: Theobromine is a major active compound in cocoa with allegedly beneficial effect on high-density-lipoprotein-cholesterol (HDL-CH). We have investigated the effect of theobromine (TB) consumption on the concentrations of triglyceride (TG) and cholesterol (CH) in various lipoprotein (LP) subclasses. Methods: In a randomized, double-blind, placebo-controlled, cross-over study, 44 apparently healthy women and men (age: 60 ± 6 years, BMI: 29 ± 3 kg/m2) with low baseline HDL-CH concentrations consumed a drink supplemented with 500 mg/d theobromine for 4 weeks. TG and CH concentrations in 15 LP subclasses were predicted from diffusion-edited 1H NMR spectra of fasting serum. Results: The LP phenotype of the subjects was characterized by low CH concentrations in the large HDL particles and high TG concentrations in large VLDL and chylomicron (CM) particles, which clearly differed from a LP phenotype of subjects with normal HDL-CH. TB only reduced CH concentrations in the LDL particles by 3.64 and 6.79{\%}, but had no effect on TG and CH in any of the HDL, VLDL and CM subclasses. Conclusion: TB was not effective on HDL-CH in subjects with a LP phenotype characterized by low HDL-CH and high TG in VLDL.",
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author = "Jacobs, {Doris M.} and Lotte Smolders and Yuguang Lin and {de Roo}, Niels and Trautwein, {Elke A.} and {van Duynhoven}, John and Mensink, {Ronald P.} and Jogchum Plat and Mihaleva, {Velitchka V.}",
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Effect of theobromine consumption on serum lipoprotein profiles in apparently healthy humans with low HDL-cholesterol concentrations. / Jacobs, Doris M.; Smolders, Lotte; Lin, Yuguang; de Roo, Niels; Trautwein, Elke A.; van Duynhoven, John; Mensink, Ronald P.; Plat, Jogchum; Mihaleva, Velitchka V.

In: Frontiers in Molecular Biosciences, Vol. 4, No. AUG, 59, 24.08.2017.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effect of theobromine consumption on serum lipoprotein profiles in apparently healthy humans with low HDL-cholesterol concentrations

AU - Jacobs, Doris M.

AU - Smolders, Lotte

AU - Lin, Yuguang

AU - de Roo, Niels

AU - Trautwein, Elke A.

AU - van Duynhoven, John

AU - Mensink, Ronald P.

AU - Plat, Jogchum

AU - Mihaleva, Velitchka V.

PY - 2017/8/24

Y1 - 2017/8/24

N2 - Scope: Theobromine is a major active compound in cocoa with allegedly beneficial effect on high-density-lipoprotein-cholesterol (HDL-CH). We have investigated the effect of theobromine (TB) consumption on the concentrations of triglyceride (TG) and cholesterol (CH) in various lipoprotein (LP) subclasses. Methods: In a randomized, double-blind, placebo-controlled, cross-over study, 44 apparently healthy women and men (age: 60 ± 6 years, BMI: 29 ± 3 kg/m2) with low baseline HDL-CH concentrations consumed a drink supplemented with 500 mg/d theobromine for 4 weeks. TG and CH concentrations in 15 LP subclasses were predicted from diffusion-edited 1H NMR spectra of fasting serum. Results: The LP phenotype of the subjects was characterized by low CH concentrations in the large HDL particles and high TG concentrations in large VLDL and chylomicron (CM) particles, which clearly differed from a LP phenotype of subjects with normal HDL-CH. TB only reduced CH concentrations in the LDL particles by 3.64 and 6.79%, but had no effect on TG and CH in any of the HDL, VLDL and CM subclasses. Conclusion: TB was not effective on HDL-CH in subjects with a LP phenotype characterized by low HDL-CH and high TG in VLDL.

AB - Scope: Theobromine is a major active compound in cocoa with allegedly beneficial effect on high-density-lipoprotein-cholesterol (HDL-CH). We have investigated the effect of theobromine (TB) consumption on the concentrations of triglyceride (TG) and cholesterol (CH) in various lipoprotein (LP) subclasses. Methods: In a randomized, double-blind, placebo-controlled, cross-over study, 44 apparently healthy women and men (age: 60 ± 6 years, BMI: 29 ± 3 kg/m2) with low baseline HDL-CH concentrations consumed a drink supplemented with 500 mg/d theobromine for 4 weeks. TG and CH concentrations in 15 LP subclasses were predicted from diffusion-edited 1H NMR spectra of fasting serum. Results: The LP phenotype of the subjects was characterized by low CH concentrations in the large HDL particles and high TG concentrations in large VLDL and chylomicron (CM) particles, which clearly differed from a LP phenotype of subjects with normal HDL-CH. TB only reduced CH concentrations in the LDL particles by 3.64 and 6.79%, but had no effect on TG and CH in any of the HDL, VLDL and CM subclasses. Conclusion: TB was not effective on HDL-CH in subjects with a LP phenotype characterized by low HDL-CH and high TG in VLDL.

KW - HDL

KW - Lipoprotein

KW - NMR

KW - PLS model

KW - Theobromine

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DO - 10.3389/fmolb.2017.00059

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ER -