Effect of the DGAT1 K232A genotype of dairy cows on the milk metabolome and proteome

J. Lu, S. Boeren, A.C.M. van Hooijdonk, J.J.M. Vervoort, K.A. Hettinga

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Diglyceride O-acyltransferase 1 (DGAT1) is the enzyme that catalyzes the synthesis of triglycerides from diglycerides and acyl-coenzyme A. The DGAT1 K232A polymorphism was previously shown to have a significant influence on bovine milk production characteristics (milk yield, protein content, fat content, and fatty acid composition). The mechanism of this influence has, however, not been elucidated. In this study, metabolomics (1H-nuclear magnetic resonance) and proteomics (laser chromatography-tandem mass spectrometry) were applied to determine the serum and lipid metabolite composition and milk fat globule membrane proteome of milk samples from cows with the DGAT1 KK and AA genotypes. The milk samples from cows with the DGAT1 KK genotype contained more stomatin, sphingomyelin, choline, and carnitine, and less citrate, creatine or phosphocreatine, glycerol-phosphocholine, mannose-like sugar, acetyl sugar phosphate, uridine diphosphate (UDP)-related sugar, and orotic acid compared with milk samples from cows with the DGAT1 AA genotype. Based on these results, we propose that the differences between the DGAT1 genotypes may be related to stomatin-sphingomyelin lipid rafts as well as structural (cell membrane) differences in epithelial cells of the mammary gland. In conclusion, our study shows that, in addition to previously described changes in triglyceride composition, cows differing in DGAT1 polymorphism differ in their milk proteome and metabolome, which may help in further understanding the effect of the DGAT1 K232A polymorphism on milk production characteristics.
LanguageEnglish
Pages3460-3469
JournalJournal of Dairy Science
Volume98
Issue number5
DOIs
Publication statusPublished - 2015

Fingerprint

Diacylglycerol O-Acyltransferase
diacylglycerol acyltransferase
metabolome
Metabolome
Proteome
proteome
Milk
dairy cows
Genotype
milk
genotype
cows
sphingomyelins
Sphingomyelins
genetic polymorphism
milk production
Triglycerides
Uridine Diphosphate Sugars
triacylglycerols
uridine diphosphate

Keywords

  • h-1-nmr spectroscopy
  • sample preparation
  • identification
  • stomatin
  • membrane
  • proteins
  • gene
  • cattle
  • yield

Cite this

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title = "Effect of the DGAT1 K232A genotype of dairy cows on the milk metabolome and proteome",
abstract = "Diglyceride O-acyltransferase 1 (DGAT1) is the enzyme that catalyzes the synthesis of triglycerides from diglycerides and acyl-coenzyme A. The DGAT1 K232A polymorphism was previously shown to have a significant influence on bovine milk production characteristics (milk yield, protein content, fat content, and fatty acid composition). The mechanism of this influence has, however, not been elucidated. In this study, metabolomics (1H-nuclear magnetic resonance) and proteomics (laser chromatography-tandem mass spectrometry) were applied to determine the serum and lipid metabolite composition and milk fat globule membrane proteome of milk samples from cows with the DGAT1 KK and AA genotypes. The milk samples from cows with the DGAT1 KK genotype contained more stomatin, sphingomyelin, choline, and carnitine, and less citrate, creatine or phosphocreatine, glycerol-phosphocholine, mannose-like sugar, acetyl sugar phosphate, uridine diphosphate (UDP)-related sugar, and orotic acid compared with milk samples from cows with the DGAT1 AA genotype. Based on these results, we propose that the differences between the DGAT1 genotypes may be related to stomatin-sphingomyelin lipid rafts as well as structural (cell membrane) differences in epithelial cells of the mammary gland. In conclusion, our study shows that, in addition to previously described changes in triglyceride composition, cows differing in DGAT1 polymorphism differ in their milk proteome and metabolome, which may help in further understanding the effect of the DGAT1 K232A polymorphism on milk production characteristics.",
keywords = "h-1-nmr spectroscopy, sample preparation, identification, stomatin, membrane, proteins, gene, cattle, yield",
author = "J. Lu and S. Boeren and {van Hooijdonk}, A.C.M. and J.J.M. Vervoort and K.A. Hettinga",
year = "2015",
doi = "10.3168/jds.2014-8872",
language = "English",
volume = "98",
pages = "3460--3469",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "American Dairy Science Association",
number = "5",

}

Effect of the DGAT1 K232A genotype of dairy cows on the milk metabolome and proteome. / Lu, J.; Boeren, S.; van Hooijdonk, A.C.M.; Vervoort, J.J.M.; Hettinga, K.A.

In: Journal of Dairy Science, Vol. 98, No. 5, 2015, p. 3460-3469.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effect of the DGAT1 K232A genotype of dairy cows on the milk metabolome and proteome

AU - Lu, J.

AU - Boeren, S.

AU - van Hooijdonk, A.C.M.

AU - Vervoort, J.J.M.

AU - Hettinga, K.A.

PY - 2015

Y1 - 2015

N2 - Diglyceride O-acyltransferase 1 (DGAT1) is the enzyme that catalyzes the synthesis of triglycerides from diglycerides and acyl-coenzyme A. The DGAT1 K232A polymorphism was previously shown to have a significant influence on bovine milk production characteristics (milk yield, protein content, fat content, and fatty acid composition). The mechanism of this influence has, however, not been elucidated. In this study, metabolomics (1H-nuclear magnetic resonance) and proteomics (laser chromatography-tandem mass spectrometry) were applied to determine the serum and lipid metabolite composition and milk fat globule membrane proteome of milk samples from cows with the DGAT1 KK and AA genotypes. The milk samples from cows with the DGAT1 KK genotype contained more stomatin, sphingomyelin, choline, and carnitine, and less citrate, creatine or phosphocreatine, glycerol-phosphocholine, mannose-like sugar, acetyl sugar phosphate, uridine diphosphate (UDP)-related sugar, and orotic acid compared with milk samples from cows with the DGAT1 AA genotype. Based on these results, we propose that the differences between the DGAT1 genotypes may be related to stomatin-sphingomyelin lipid rafts as well as structural (cell membrane) differences in epithelial cells of the mammary gland. In conclusion, our study shows that, in addition to previously described changes in triglyceride composition, cows differing in DGAT1 polymorphism differ in their milk proteome and metabolome, which may help in further understanding the effect of the DGAT1 K232A polymorphism on milk production characteristics.

AB - Diglyceride O-acyltransferase 1 (DGAT1) is the enzyme that catalyzes the synthesis of triglycerides from diglycerides and acyl-coenzyme A. The DGAT1 K232A polymorphism was previously shown to have a significant influence on bovine milk production characteristics (milk yield, protein content, fat content, and fatty acid composition). The mechanism of this influence has, however, not been elucidated. In this study, metabolomics (1H-nuclear magnetic resonance) and proteomics (laser chromatography-tandem mass spectrometry) were applied to determine the serum and lipid metabolite composition and milk fat globule membrane proteome of milk samples from cows with the DGAT1 KK and AA genotypes. The milk samples from cows with the DGAT1 KK genotype contained more stomatin, sphingomyelin, choline, and carnitine, and less citrate, creatine or phosphocreatine, glycerol-phosphocholine, mannose-like sugar, acetyl sugar phosphate, uridine diphosphate (UDP)-related sugar, and orotic acid compared with milk samples from cows with the DGAT1 AA genotype. Based on these results, we propose that the differences between the DGAT1 genotypes may be related to stomatin-sphingomyelin lipid rafts as well as structural (cell membrane) differences in epithelial cells of the mammary gland. In conclusion, our study shows that, in addition to previously described changes in triglyceride composition, cows differing in DGAT1 polymorphism differ in their milk proteome and metabolome, which may help in further understanding the effect of the DGAT1 K232A polymorphism on milk production characteristics.

KW - h-1-nmr spectroscopy

KW - sample preparation

KW - identification

KW - stomatin

KW - membrane

KW - proteins

KW - gene

KW - cattle

KW - yield

U2 - 10.3168/jds.2014-8872

DO - 10.3168/jds.2014-8872

M3 - Article

VL - 98

SP - 3460

EP - 3469

JO - Journal of Dairy Science

T2 - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 5

ER -