Effect of sucrose on adventitious root regeneration in apple

A. Calamar; G.J.M. de Klerk

doi:10.1023/A:1016356123367

Effect of sucrose on adventitious root regeneration in apple

A. Calamar, G.J.M. de Klerk

Research output: Contribution to journal › Article › Academic › peer-review

57 Citations (Scopus)

Abstract

We have examined the effect of sucrose on adventitious root formation in apple microcuttings and in 1-mm stem slices cut from apple microcuttings. The sucrose concentration influenced the number of adventitious roots, but at a broad range of sucrose concentrations (1¿9%) the effect was small. In addition, there was an interaction between sucrose and auxin: increasing the sucrose concentration shifted the dose¿response curve of auxin to the right. When slices were cultured on medium without sucrose for the initial period (0¿48 h), rooting was reduced whereas 48-h culture without application of sucrose had hardly any effect or even a slight promotive effect in a later period (48¿120 h). The results show that during adventitious root formation, applied sucrose is used as a source of energy and building blocks but they are also in accordance with a possible regulatory role of sucrose

Original language	English
Pages (from-to)	207-212
Journal	Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
Volume	70
Issue number	2
DOIs	https://doi.org/10.1023/A:1016356123367
Publication status	Published - 2002

Keywords

forming tobacco callus
somatic embryogenesis
developing cotyledons
starch accumulation
auxin
part

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title = "Effect of sucrose on adventitious root regeneration in apple",

abstract = "We have examined the effect of sucrose on adventitious root formation in apple microcuttings and in 1-mm stem slices cut from apple microcuttings. The sucrose concentration influenced the number of adventitious roots, but at a broad range of sucrose concentrations (1¿9%) the effect was small. In addition, there was an interaction between sucrose and auxin: increasing the sucrose concentration shifted the dose¿response curve of auxin to the right. When slices were cultured on medium without sucrose for the initial period (0¿48 h), rooting was reduced whereas 48-h culture without application of sucrose had hardly any effect or even a slight promotive effect in a later period (48¿120 h). The results show that during adventitious root formation, applied sucrose is used as a source of energy and building blocks but they are also in accordance with a possible regulatory role of sucrose",

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TY - JOUR

T1 - Effect of sucrose on adventitious root regeneration in apple

AU - Calamar, A.

AU - de Klerk, G.J.M.

N1 - 000176817600011

PY - 2002

Y1 - 2002

N2 - We have examined the effect of sucrose on adventitious root formation in apple microcuttings and in 1-mm stem slices cut from apple microcuttings. The sucrose concentration influenced the number of adventitious roots, but at a broad range of sucrose concentrations (1¿9%) the effect was small. In addition, there was an interaction between sucrose and auxin: increasing the sucrose concentration shifted the dose¿response curve of auxin to the right. When slices were cultured on medium without sucrose for the initial period (0¿48 h), rooting was reduced whereas 48-h culture without application of sucrose had hardly any effect or even a slight promotive effect in a later period (48¿120 h). The results show that during adventitious root formation, applied sucrose is used as a source of energy and building blocks but they are also in accordance with a possible regulatory role of sucrose

AB - We have examined the effect of sucrose on adventitious root formation in apple microcuttings and in 1-mm stem slices cut from apple microcuttings. The sucrose concentration influenced the number of adventitious roots, but at a broad range of sucrose concentrations (1¿9%) the effect was small. In addition, there was an interaction between sucrose and auxin: increasing the sucrose concentration shifted the dose¿response curve of auxin to the right. When slices were cultured on medium without sucrose for the initial period (0¿48 h), rooting was reduced whereas 48-h culture without application of sucrose had hardly any effect or even a slight promotive effect in a later period (48¿120 h). The results show that during adventitious root formation, applied sucrose is used as a source of energy and building blocks but they are also in accordance with a possible regulatory role of sucrose

KW - forming tobacco callus

KW - somatic embryogenesis

KW - developing cotyledons

KW - starch accumulation

KW - auxin

KW - part

U2 - 10.1023/A:1016356123367

DO - 10.1023/A:1016356123367

M3 - Article

VL - 70

SP - 207

EP - 212

JO - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants

JF - Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants

SN - 0167-6857

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ER -