Effect of sucrose on adventitious root regeneration in apple

A. Calamar, G.J.M. de Klerk

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    57 Citations (Scopus)

    Abstract

    We have examined the effect of sucrose on adventitious root formation in apple microcuttings and in 1-mm stem slices cut from apple microcuttings. The sucrose concentration influenced the number of adventitious roots, but at a broad range of sucrose concentrations (1¿9%) the effect was small. In addition, there was an interaction between sucrose and auxin: increasing the sucrose concentration shifted the dose¿response curve of auxin to the right. When slices were cultured on medium without sucrose for the initial period (0¿48 h), rooting was reduced whereas 48-h culture without application of sucrose had hardly any effect or even a slight promotive effect in a later period (48¿120 h). The results show that during adventitious root formation, applied sucrose is used as a source of energy and building blocks but they are also in accordance with a possible regulatory role of sucrose
    Original languageEnglish
    Pages (from-to)207-212
    JournalPlant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants
    Volume70
    Issue number2
    DOIs
    Publication statusPublished - 2002

    Keywords

    • forming tobacco callus
    • somatic embryogenesis
    • developing cotyledons
    • starch accumulation
    • auxin
    • part

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