Effect of sample pre-treatment on the determination of steroid esters in hair of bovine calves

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Abstract

The effect of three sample pre-treatment steps, washing, cutting and grinding on the determination of steroid esters in hair is studied. The study is performed by using hair samples obtained after pour-on application of steroid esters to bovine calves. After sample pre-treatment the hair is treated with a mild reducing agent [tris(2-carboxyethyl)phosphine hydrochloride] to extract the steroid esters. After a solid-phase extraction clean-up step the extracts are analysed by using liquid chromatography combined with triple–quadrupole mass spectrometric detection. For the washing step the use of non-organic washing solvents like (warm) water and a solution of 0.1% sodium dodecyl phosphate and organic solutions containing different percentages of methanol are tested. By using the non-organic solvents and the organic solvents with a percentage of methanol
Original languageEnglish
Pages (from-to)8233-8239
JournalJournal of Chromatography. A, Including electrophoresis and other separation methods
Volume1216
Issue number46
DOIs
Publication statusPublished - 2009

Keywords

  • tandem mass-spectrometry
  • drugs

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