Abstract
The effect of three sample pre-treatment steps, washing, cutting and grinding on the determination of steroid esters in hair is studied. The study is performed by using hair samples obtained after pour-on application of steroid esters to bovine calves. After sample pre-treatment the hair is treated with a mild reducing agent [tris(2-carboxyethyl)phosphine hydrochloride] to extract the steroid esters. After a solid-phase extraction clean-up step the extracts are analysed by using liquid chromatography combined with triple–quadrupole mass spectrometric detection. For the washing step the use of non-organic washing solvents like (warm) water and a solution of 0.1% sodium dodecyl phosphate and organic solutions containing different percentages of methanol are tested. By using the non-organic solvents and the organic solvents with a percentage of methanol
| Original language | English |
|---|---|
| Pages (from-to) | 8233-8239 |
| Journal | Journal of Chromatography. A, Including electrophoresis and other separation methods |
| Volume | 1216 |
| Issue number | 46 |
| DOIs | |
| Publication status | Published - 2009 |
Keywords
- tandem mass-spectrometry
- drugs