TY - JOUR
T1 - Effect of salinity on nitrification efficiency and structure of ammonia-oxidizing bacterial communities in a submerged fixed bed bioreactor
AU - Cortés-Lorenzo, C.
AU - Rodríguez-Díaz, M.
AU - Sipkema, D.
AU - Juárez-Jiménez, B.
AU - Rodelas, B.
AU - Smidt, H.
AU - González-López, J.
PY - 2015
Y1 - 2015
N2 - The effect of salt (NaCl) on biological nitrogen removal and community structure of ammonia-oxidizing bacteria (AOB) was investigated in a submerged fixed bed bioreactor (SFBBR). Influent wastewater was supplemented with NaCl at 0 (control), 3.7, 24.1 and 44.1 g/L, and the rate of ammonia removal efficiency was measured by ion chromatography. The structure of the AOB community was profiled by 454-pyrosequencing, based on the amplification of partial ammonia-monooxygenase subunit A (amoA) genes. Salinity did not inhibit nitrification at 3.7 g/L, while ammonia oxidation activity significantly decreased and nitrite was consequently accumulated in the SFBBR when the salt concentration was ¿24.1 g/L. The sequencing of amoA genes revealed that many of the OTUs found in the control experiment were still present at the full range of NaCl studied, while concentrations of 24.1 and 44.1 g of NaCl/L promoted the emergence of new OTUs phylogenetically related to AOB described in saline environments
AB - The effect of salt (NaCl) on biological nitrogen removal and community structure of ammonia-oxidizing bacteria (AOB) was investigated in a submerged fixed bed bioreactor (SFBBR). Influent wastewater was supplemented with NaCl at 0 (control), 3.7, 24.1 and 44.1 g/L, and the rate of ammonia removal efficiency was measured by ion chromatography. The structure of the AOB community was profiled by 454-pyrosequencing, based on the amplification of partial ammonia-monooxygenase subunit A (amoA) genes. Salinity did not inhibit nitrification at 3.7 g/L, while ammonia oxidation activity significantly decreased and nitrite was consequently accumulated in the SFBBR when the salt concentration was ¿24.1 g/L. The sequencing of amoA genes revealed that many of the OTUs found in the control experiment were still present at the full range of NaCl studied, while concentrations of 24.1 and 44.1 g of NaCl/L promoted the emergence of new OTUs phylogenetically related to AOB described in saline environments
KW - 454-Pyrosequencing
KW - Ammonia-oxidizing bacteria
KW - Biological nitrogen removal
KW - Saline wastewater
KW - Submerged fixed bed bioreactor
U2 - 10.1016/j.cej.2014.12.083
DO - 10.1016/j.cej.2014.12.083
M3 - Article
SN - 1385-8947
VL - 266
SP - 233
EP - 240
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
ER -