Effect of removal of bacteria on the biomass and extracellular carbohydrate productivity of Botryococcus braunii

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Abstract

Botryococcus braunii CCALA778 is a green microalga that can produce large amounts of extracellular carbohydrates and therefore is a potential host for industrial applications such as materials, food and pharmaceutical products. The downside of B. braunii is its slow growth and therefore, improvements on the biomass productivity or carbohydrate production will make this microalga more attractive for industrial exploitation. Microalgae grow naturally in the presence of bacteria and these can be beneficial or antagonistic. In outdoor cultivation systems, contamination by bacteria is common. The role or effects of bacteria present in B. braunii CCALA778 are not yet fully elucidated. We used UV-C treatment to reduce bacterial abundance in B. braunii CCALA778 cultures and 16S rRNA gene amplicon MiSeq sequencing for bacterial community analysis. The effect of the reduced amount of bacteria on biomass growth and production of extracellular carbohydrates was analysed. It is shown that UV-C treatment can reduce the bacterial population substantially without harming B. braunii. Bacteria removed by UV-C were antagonistic to B. braunii CCALA778 as in their absence production of biomass and extracellular carbohydrates was enhanced significantly. CCALA778 treated with UV-C accumulated 826 ± 61 mg L−1 of extracellular carbohydrates by day 15 compared with 422 ± 135 mg L−1 accumulated extracellular carbohydrates in the untreated culture.

LanguageEnglish
Number of pages11
JournalJournal of Applied Phycology
DOIs
Publication statusE-pub ahead of print - 26 Jun 2019

Fingerprint

carbohydrate
carbohydrates
productivity
bacterium
biomass
bacteria
microalga
industrial applications
microalgae
bacterial communities
removal
effect
Botryococcus braunii
biomass production
foods
drug
ribosomal RNA
drugs
food
gene

Keywords

  • 16S analysis
  • Bacteria
  • Botryococcus braunii
  • Chlorophyceae
  • Exopolysaccharides
  • Microalgae
  • UV light

Cite this

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title = "Effect of removal of bacteria on the biomass and extracellular carbohydrate productivity of Botryococcus braunii",
abstract = "Botryococcus braunii CCALA778 is a green microalga that can produce large amounts of extracellular carbohydrates and therefore is a potential host for industrial applications such as materials, food and pharmaceutical products. The downside of B. braunii is its slow growth and therefore, improvements on the biomass productivity or carbohydrate production will make this microalga more attractive for industrial exploitation. Microalgae grow naturally in the presence of bacteria and these can be beneficial or antagonistic. In outdoor cultivation systems, contamination by bacteria is common. The role or effects of bacteria present in B. braunii CCALA778 are not yet fully elucidated. We used UV-C treatment to reduce bacterial abundance in B. braunii CCALA778 cultures and 16S rRNA gene amplicon MiSeq sequencing for bacterial community analysis. The effect of the reduced amount of bacteria on biomass growth and production of extracellular carbohydrates was analysed. It is shown that UV-C treatment can reduce the bacterial population substantially without harming B. braunii. Bacteria removed by UV-C were antagonistic to B. braunii CCALA778 as in their absence production of biomass and extracellular carbohydrates was enhanced significantly. CCALA778 treated with UV-C accumulated 826 ± 61 mg L−1 of extracellular carbohydrates by day 15 compared with 422 ± 135 mg L−1 accumulated extracellular carbohydrates in the untreated culture.",
keywords = "16S analysis, Bacteria, Botryococcus braunii, Chlorophyceae, Exopolysaccharides, Microalgae, UV light",
author = "Gouveia, {Joao D.} and Antoine Moers and Yvonne Griekspoor and {van den Broek}, {Lambertus A.M.} and Jan Springer and Lolke Sijtsma and Detmer Sipkema and Wijffels, {Rene H.} and Barbosa, {Maria J.}",
year = "2019",
month = "6",
day = "26",
doi = "10.1007/s10811-019-01847-0",
language = "English",
journal = "Journal of Applied Phycology",
issn = "0921-8971",
publisher = "Springer Verlag",

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TY - JOUR

T1 - Effect of removal of bacteria on the biomass and extracellular carbohydrate productivity of Botryococcus braunii

AU - Gouveia, Joao D.

AU - Moers, Antoine

AU - Griekspoor, Yvonne

AU - van den Broek, Lambertus A.M.

AU - Springer, Jan

AU - Sijtsma, Lolke

AU - Sipkema, Detmer

AU - Wijffels, Rene H.

AU - Barbosa, Maria J.

PY - 2019/6/26

Y1 - 2019/6/26

N2 - Botryococcus braunii CCALA778 is a green microalga that can produce large amounts of extracellular carbohydrates and therefore is a potential host for industrial applications such as materials, food and pharmaceutical products. The downside of B. braunii is its slow growth and therefore, improvements on the biomass productivity or carbohydrate production will make this microalga more attractive for industrial exploitation. Microalgae grow naturally in the presence of bacteria and these can be beneficial or antagonistic. In outdoor cultivation systems, contamination by bacteria is common. The role or effects of bacteria present in B. braunii CCALA778 are not yet fully elucidated. We used UV-C treatment to reduce bacterial abundance in B. braunii CCALA778 cultures and 16S rRNA gene amplicon MiSeq sequencing for bacterial community analysis. The effect of the reduced amount of bacteria on biomass growth and production of extracellular carbohydrates was analysed. It is shown that UV-C treatment can reduce the bacterial population substantially without harming B. braunii. Bacteria removed by UV-C were antagonistic to B. braunii CCALA778 as in their absence production of biomass and extracellular carbohydrates was enhanced significantly. CCALA778 treated with UV-C accumulated 826 ± 61 mg L−1 of extracellular carbohydrates by day 15 compared with 422 ± 135 mg L−1 accumulated extracellular carbohydrates in the untreated culture.

AB - Botryococcus braunii CCALA778 is a green microalga that can produce large amounts of extracellular carbohydrates and therefore is a potential host for industrial applications such as materials, food and pharmaceutical products. The downside of B. braunii is its slow growth and therefore, improvements on the biomass productivity or carbohydrate production will make this microalga more attractive for industrial exploitation. Microalgae grow naturally in the presence of bacteria and these can be beneficial or antagonistic. In outdoor cultivation systems, contamination by bacteria is common. The role or effects of bacteria present in B. braunii CCALA778 are not yet fully elucidated. We used UV-C treatment to reduce bacterial abundance in B. braunii CCALA778 cultures and 16S rRNA gene amplicon MiSeq sequencing for bacterial community analysis. The effect of the reduced amount of bacteria on biomass growth and production of extracellular carbohydrates was analysed. It is shown that UV-C treatment can reduce the bacterial population substantially without harming B. braunii. Bacteria removed by UV-C were antagonistic to B. braunii CCALA778 as in their absence production of biomass and extracellular carbohydrates was enhanced significantly. CCALA778 treated with UV-C accumulated 826 ± 61 mg L−1 of extracellular carbohydrates by day 15 compared with 422 ± 135 mg L−1 accumulated extracellular carbohydrates in the untreated culture.

KW - 16S analysis

KW - Bacteria

KW - Botryococcus braunii

KW - Chlorophyceae

KW - Exopolysaccharides

KW - Microalgae

KW - UV light

U2 - 10.1007/s10811-019-01847-0

DO - 10.1007/s10811-019-01847-0

M3 - Article

JO - Journal of Applied Phycology

T2 - Journal of Applied Phycology

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SN - 0921-8971

ER -