Effect of (+)-pulegone and other oil components of Mentha x piperita on cucumber respiration

M. Mucciarelli, W. Camusso, C.M. Bertea, S. Bossi, M. Maffei

    Research output: Contribution to journalArticleAcademicpeer-review

    49 Citations (Scopus)

    Abstract

    Peppermint (Menthaxpiperita L.) essential oil and main components were assessed for their ability to interfere with plant respiratory functions. Tests were conducted on both root segments and mitochondria isolated by etiolated seedlings of cucumber (Cucumis sativus L.). Total essential oil inhibited 50% of root and mitochondrial respiration (IC50) when used at 324 and 593 ppm, respectively. ( )-Pulegone was the most toxic compound, with a 0.08 and 0.12 mM IC50 for root and mitochondrial respiration, respectively. (−)-Menthone, followed ( )-pulegone in its inhibitory action (IC50 values of 1.11 and 2.30 mM for root and mitochondrial respiration respectively), whereas (−)-menthol was the less inhibitory compound (IC50 values of 1.85 and 3.80 mM respectively). A positive correlation was found for ( )-pulegone, (−)-menthone and (−)-menthol between water solubility and respiratory inhibition. The uncoupling agent, carbonyl-cyanide-m-chlorophenyl-hydrazone (CCCP), lowered (−)-menthol and (−)-menthone inhibition and annulled ( )-pulegone inhibition of mitochondrial respiration, whereas salicyl-hydroxamic acid (SHAM) 2-hydroxybenzohydroxamic acid, the alternative oxidase (AO) inhibitor, increased (−)-menthone inhibition and annulled both ( )-pulegone and (−)-menthol inhibitory activity. The possible interaction of ( )-pulegone and (−)-menthol with AO and the mechanism of action of ( )-pulegone, (−)-menthone and (−)-menthol on mitochondrial respiration are discussed.
    Original languageEnglish
    Pages (from-to)91-98
    JournalPhytochemistry
    Volume57
    DOIs
    Publication statusPublished - 2001

    Fingerprint

    Mentha piperita
    Cucumis sativus
    menthol
    Mentha
    Menthol
    menthone
    cucumbers
    breathing
    Respiration
    Oils
    oils
    inhibitory concentration 50
    Inhibitory Concentration 50
    Volatile Oils
    essential oils
    hydroxamic acids
    Uncoupling Agents
    Carbonyl Cyanide m-Chlorophenyl Hydrazone
    Mentha piperita nothosubsp. piperita
    Hydroxamic Acids

    Cite this

    Mucciarelli, M. ; Camusso, W. ; Bertea, C.M. ; Bossi, S. ; Maffei, M. / Effect of (+)-pulegone and other oil components of Mentha x piperita on cucumber respiration. In: Phytochemistry. 2001 ; Vol. 57. pp. 91-98.
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    abstract = "Peppermint (Menthaxpiperita L.) essential oil and main components were assessed for their ability to interfere with plant respiratory functions. Tests were conducted on both root segments and mitochondria isolated by etiolated seedlings of cucumber (Cucumis sativus L.). Total essential oil inhibited 50{\%} of root and mitochondrial respiration (IC50) when used at 324 and 593 ppm, respectively. ( )-Pulegone was the most toxic compound, with a 0.08 and 0.12 mM IC50 for root and mitochondrial respiration, respectively. (−)-Menthone, followed ( )-pulegone in its inhibitory action (IC50 values of 1.11 and 2.30 mM for root and mitochondrial respiration respectively), whereas (−)-menthol was the less inhibitory compound (IC50 values of 1.85 and 3.80 mM respectively). A positive correlation was found for ( )-pulegone, (−)-menthone and (−)-menthol between water solubility and respiratory inhibition. The uncoupling agent, carbonyl-cyanide-m-chlorophenyl-hydrazone (CCCP), lowered (−)-menthol and (−)-menthone inhibition and annulled ( )-pulegone inhibition of mitochondrial respiration, whereas salicyl-hydroxamic acid (SHAM) 2-hydroxybenzohydroxamic acid, the alternative oxidase (AO) inhibitor, increased (−)-menthone inhibition and annulled both ( )-pulegone and (−)-menthol inhibitory activity. The possible interaction of ( )-pulegone and (−)-menthol with AO and the mechanism of action of ( )-pulegone, (−)-menthone and (−)-menthol on mitochondrial respiration are discussed.",
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    Effect of (+)-pulegone and other oil components of Mentha x piperita on cucumber respiration. / Mucciarelli, M.; Camusso, W.; Bertea, C.M.; Bossi, S.; Maffei, M.

    In: Phytochemistry, Vol. 57, 2001, p. 91-98.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Effect of (+)-pulegone and other oil components of Mentha x piperita on cucumber respiration

    AU - Mucciarelli, M.

    AU - Camusso, W.

    AU - Bertea, C.M.

    AU - Bossi, S.

    AU - Maffei, M.

    PY - 2001

    Y1 - 2001

    N2 - Peppermint (Menthaxpiperita L.) essential oil and main components were assessed for their ability to interfere with plant respiratory functions. Tests were conducted on both root segments and mitochondria isolated by etiolated seedlings of cucumber (Cucumis sativus L.). Total essential oil inhibited 50% of root and mitochondrial respiration (IC50) when used at 324 and 593 ppm, respectively. ( )-Pulegone was the most toxic compound, with a 0.08 and 0.12 mM IC50 for root and mitochondrial respiration, respectively. (−)-Menthone, followed ( )-pulegone in its inhibitory action (IC50 values of 1.11 and 2.30 mM for root and mitochondrial respiration respectively), whereas (−)-menthol was the less inhibitory compound (IC50 values of 1.85 and 3.80 mM respectively). A positive correlation was found for ( )-pulegone, (−)-menthone and (−)-menthol between water solubility and respiratory inhibition. The uncoupling agent, carbonyl-cyanide-m-chlorophenyl-hydrazone (CCCP), lowered (−)-menthol and (−)-menthone inhibition and annulled ( )-pulegone inhibition of mitochondrial respiration, whereas salicyl-hydroxamic acid (SHAM) 2-hydroxybenzohydroxamic acid, the alternative oxidase (AO) inhibitor, increased (−)-menthone inhibition and annulled both ( )-pulegone and (−)-menthol inhibitory activity. The possible interaction of ( )-pulegone and (−)-menthol with AO and the mechanism of action of ( )-pulegone, (−)-menthone and (−)-menthol on mitochondrial respiration are discussed.

    AB - Peppermint (Menthaxpiperita L.) essential oil and main components were assessed for their ability to interfere with plant respiratory functions. Tests were conducted on both root segments and mitochondria isolated by etiolated seedlings of cucumber (Cucumis sativus L.). Total essential oil inhibited 50% of root and mitochondrial respiration (IC50) when used at 324 and 593 ppm, respectively. ( )-Pulegone was the most toxic compound, with a 0.08 and 0.12 mM IC50 for root and mitochondrial respiration, respectively. (−)-Menthone, followed ( )-pulegone in its inhibitory action (IC50 values of 1.11 and 2.30 mM for root and mitochondrial respiration respectively), whereas (−)-menthol was the less inhibitory compound (IC50 values of 1.85 and 3.80 mM respectively). A positive correlation was found for ( )-pulegone, (−)-menthone and (−)-menthol between water solubility and respiratory inhibition. The uncoupling agent, carbonyl-cyanide-m-chlorophenyl-hydrazone (CCCP), lowered (−)-menthol and (−)-menthone inhibition and annulled ( )-pulegone inhibition of mitochondrial respiration, whereas salicyl-hydroxamic acid (SHAM) 2-hydroxybenzohydroxamic acid, the alternative oxidase (AO) inhibitor, increased (−)-menthone inhibition and annulled both ( )-pulegone and (−)-menthol inhibitory activity. The possible interaction of ( )-pulegone and (−)-menthol with AO and the mechanism of action of ( )-pulegone, (−)-menthone and (−)-menthol on mitochondrial respiration are discussed.

    U2 - 10.1016/S0031-9422(00)00393-9

    DO - 10.1016/S0031-9422(00)00393-9

    M3 - Article

    VL - 57

    SP - 91

    EP - 98

    JO - Phytochemistry

    JF - Phytochemistry

    SN - 0031-9422

    ER -