Effect of prior drought and pathogen stress on Arabidopsis transcriptome changes to caterpillar herbivory

Nelson H. Davila Olivas, Silvia Coolen, Pingping Huang, Edouard Severing, Marcel C. van Verk, Richard Hickman, Alexander H.J. Wittenberg, Martin de Vos, Marcel Prins, Joop J.A. van Loon, Mark G.M. Aarts, Saskia C.M. van Wees, Corné M.J. Pieterse, Marcel Dicke*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

30 Citations (Scopus)

Abstract

In nature, plants are exposed to biotic and abiotic stresses that often occur simultaneously. Therefore, plant responses to combinations of stresses are most representative of how plants respond to stresses. We used RNAseq to assess temporal changes in the transcriptome of Arabidopsis thaliana to herbivory by Pieris rapae caterpillars, either alone or in combination with prior exposure to drought or infection with the necrotrophic fungus Botrytis cinerea. Pre-exposure to drought stress or Botrytis infection resulted in a significantly different timing of the caterpillar-induced transcriptional changes. Additionally, the combination of drought and P. rapae induced an extensive downregulation of A. thaliana genes involved in defence against pathogens. Despite a more substantial growth reduction observed for plants exposed to drought plus P. rapae feeding compared with P. rapae feeding alone, this did not affect weight increase of this specialist caterpillar. Plants respond to combined stresses with phenotypic and transcriptional changes that differ from the single stress situation. The effect of a previous exposure to drought or B. cinerea infection on transcriptional changes to caterpillars is largely overridden by the stress imposed by caterpillars, indicating that plants shift their response to the most recent stress applied.

Original languageEnglish
Pages (from-to)1344-1356
JournalNew Phytologist
Volume210
Issue number4
DOIs
Publication statusPublished - 2016

Keywords

  • Abiotic stress
  • Botrytis cinerea
  • Combined stresses
  • Insect herbivory
  • Multiple stresses
  • Pieris rapae
  • RNAseq
  • Transcriptome
  • 016-3939

Fingerprint

Dive into the research topics of 'Effect of prior drought and pathogen stress on Arabidopsis transcriptome changes to caterpillar herbivory'. Together they form a unique fingerprint.

Cite this