Effect of Oxidative Enzymes on Bulk Rheological Properties of Wheat Flour Doughs

B. Dunnewind, T. van Vliet, R. Orsel

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62 Citations (Scopus)


The use of enzymes such as peroxidases or glucose oxidase instead of chemical oxidants is a very interesting option for improving breadmaking performance of doughs. In this study the effect of such enzymes on bulk rheological properties of dough was quantified and their influence on the polymer network in dough deduced. Small deformation oscillation and relaxation tests (strain 0·001) are not suitable for discriminating between doughs prepared in the presence of the different enzymes. Flow relaxation tests at high deformation and long relaxation times showed a clear distinction between the effect of peroxidase and glucose oxidase. Peroxidase increases only the number or lifetime of transient bonds, whereas glucose oxidase additionally produced cross-links that were permanent on time scales up to 3 h. Peroxidase probably introduced a second, more transient structure (arabinoxylan network) through the gluten network, whereas glucose oxidase may also have strengthened the gluten network. A higher water addition could not compensate for the effect of peroxidase; on longer time scales the stress remained at a higher level. Similar results were obtained in large deformation biaxial and uniaxial extension tests. Peroxidases only increased stress levels. The addition of glucose oxidase resulted in a higher stress and more intense strain hardening. Only in biaxial extension was an influence of pH observed. An increase in stress level was accompanied by a decrease in fracture strain, making predictions of the effects on bread structure complicated. Author Keywords: dough rheology, relaxation, peroxidase, glucose oxidase
Original languageEnglish
Pages (from-to)357-366
JournalJournal of Cereal Science
Issue number3
Publication statusPublished - 2002


  • water-soluble pentosans
  • glucose-oxidase
  • ferulic acid
  • gelation
  • gluten
  • performance


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