Effect of O2:CO2 Ratio on the Primary Metabolism of Chlamydomonas reinhardtii

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Abstract

High oxygen:carbon dioxide ratios may have a negative effect on growth and productivity of microalgae. To investigate the effect of O2 and CO2 concentrations and the ratio between these on the metabolism of Chlamydomonas reinhardtii we performed turbidostat experiments at different O2:CO2 ratios. These experiments showed that elevated O2 concentrations and the corresponding increase in the ratio of O2:CO2 common in photobioreactors led to a reduction of growth and biomass yield on light with 20–30%. This is most probably related to the oxygenase activity of Rubisco and the resulting process of photorespiration. Using metabolic flux modeling with measured rates for each experiment we were able to quantify the ratio of the oxygenase reaction to the carboxylase reaction of Rubisco and could demonstrate that photorespiration indeed can cause the reduction in biomass yield on light. The calculated ratio of the oxygenase reaction to the carboxylase reaction was 16.6% and 20.5% for air with 2% CO2 and 1% CO2, respectively. Thus photorespiration has a significant impact on the biomass yield on light already at conditions common in photobioreactors (air with 2% CO2).
Original languageEnglish
Pages (from-to)2390-2402
JournalBiotechnology and Bioengineering
Volume108
Issue number10
DOIs
Publication statusPublished - 2011

Fingerprint

Chlamydomonas reinhardtii
Oxygenases
Photobioreactors
Metabolism
Biomass
Ribulose-Bisphosphate Carboxylase
Light
Air
Microalgae
Experiments
Growth
Carbon Dioxide
Carbon dioxide
Productivity
Oxygen
Fluxes

Keywords

  • quantum requirement
  • energy-production
  • escherichia-coli
  • photosynthesis
  • light
  • microalgae
  • growth
  • pathways
  • yield
  • photobioreactors

Cite this

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title = "Effect of O2:CO2 Ratio on the Primary Metabolism of Chlamydomonas reinhardtii",
abstract = "High oxygen:carbon dioxide ratios may have a negative effect on growth and productivity of microalgae. To investigate the effect of O2 and CO2 concentrations and the ratio between these on the metabolism of Chlamydomonas reinhardtii we performed turbidostat experiments at different O2:CO2 ratios. These experiments showed that elevated O2 concentrations and the corresponding increase in the ratio of O2:CO2 common in photobioreactors led to a reduction of growth and biomass yield on light with 20–30{\%}. This is most probably related to the oxygenase activity of Rubisco and the resulting process of photorespiration. Using metabolic flux modeling with measured rates for each experiment we were able to quantify the ratio of the oxygenase reaction to the carboxylase reaction of Rubisco and could demonstrate that photorespiration indeed can cause the reduction in biomass yield on light. The calculated ratio of the oxygenase reaction to the carboxylase reaction was 16.6{\%} and 20.5{\%} for air with 2{\%} CO2 and 1{\%} CO2, respectively. Thus photorespiration has a significant impact on the biomass yield on light already at conditions common in photobioreactors (air with 2{\%} CO2).",
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author = "A.M.J. Kliphuis and D.E. Martens and M.G.J. Janssen and R.H. Wijffels",
year = "2011",
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Effect of O2:CO2 Ratio on the Primary Metabolism of Chlamydomonas reinhardtii. / Kliphuis, A.M.J.; Martens, D.E.; Janssen, M.G.J.; Wijffels, R.H.

In: Biotechnology and Bioengineering, Vol. 108, No. 10, 2011, p. 2390-2402.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effect of O2:CO2 Ratio on the Primary Metabolism of Chlamydomonas reinhardtii

AU - Kliphuis, A.M.J.

AU - Martens, D.E.

AU - Janssen, M.G.J.

AU - Wijffels, R.H.

PY - 2011

Y1 - 2011

N2 - High oxygen:carbon dioxide ratios may have a negative effect on growth and productivity of microalgae. To investigate the effect of O2 and CO2 concentrations and the ratio between these on the metabolism of Chlamydomonas reinhardtii we performed turbidostat experiments at different O2:CO2 ratios. These experiments showed that elevated O2 concentrations and the corresponding increase in the ratio of O2:CO2 common in photobioreactors led to a reduction of growth and biomass yield on light with 20–30%. This is most probably related to the oxygenase activity of Rubisco and the resulting process of photorespiration. Using metabolic flux modeling with measured rates for each experiment we were able to quantify the ratio of the oxygenase reaction to the carboxylase reaction of Rubisco and could demonstrate that photorespiration indeed can cause the reduction in biomass yield on light. The calculated ratio of the oxygenase reaction to the carboxylase reaction was 16.6% and 20.5% for air with 2% CO2 and 1% CO2, respectively. Thus photorespiration has a significant impact on the biomass yield on light already at conditions common in photobioreactors (air with 2% CO2).

AB - High oxygen:carbon dioxide ratios may have a negative effect on growth and productivity of microalgae. To investigate the effect of O2 and CO2 concentrations and the ratio between these on the metabolism of Chlamydomonas reinhardtii we performed turbidostat experiments at different O2:CO2 ratios. These experiments showed that elevated O2 concentrations and the corresponding increase in the ratio of O2:CO2 common in photobioreactors led to a reduction of growth and biomass yield on light with 20–30%. This is most probably related to the oxygenase activity of Rubisco and the resulting process of photorespiration. Using metabolic flux modeling with measured rates for each experiment we were able to quantify the ratio of the oxygenase reaction to the carboxylase reaction of Rubisco and could demonstrate that photorespiration indeed can cause the reduction in biomass yield on light. The calculated ratio of the oxygenase reaction to the carboxylase reaction was 16.6% and 20.5% for air with 2% CO2 and 1% CO2, respectively. Thus photorespiration has a significant impact on the biomass yield on light already at conditions common in photobioreactors (air with 2% CO2).

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KW - energy-production

KW - escherichia-coli

KW - photosynthesis

KW - light

KW - microalgae

KW - growth

KW - pathways

KW - yield

KW - photobioreactors

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