The effect of omitting nickel from the influent on methanol conversion in an Upflow Anaerobic Sludge Bed (UASB) reactor was investigated. The UASB reactor (30°C, pH 7) was operated for 261 days at a 12-h hydraulic retention time (HRT) and at organic loading rates (OLRs) ranging from 2.6 to 7.8 g COD l reactorm1 daym1. The nickel content of the sludge decreased by 66 during the 261-day reactor run because of washout and doubling of the sludge bed volume. Nickel deprivation initially had a strong impact on the methanogenic activity of the sludge with methanol; e.g., after 89 days of operation, this activity was doubled by adding 2 wM nickel. Upon prolonged UASB reactor operation, methanol and VFA effluent concentrations decreased whereas the sludge lost its response to nickel addition in activity tests. This suggests that a less nickel-dependent methanol-converting sludge had developed in the UASB reactor.
|Journal||Journal of Industrial Microbiology and Biotechnology|
|Publication status||Published - 2002|
Zandvoort, M. H., Osuna, M. B., Geerts, R., Lettinga, G., & Lens, P. N. L. (2002). Effect of nickel deprivation on methanol degradation in a methanogenic granular sludge bioreactor. Journal of Industrial Microbiology and Biotechnology, 29, 268-274. https://doi.org/10.1038/sj.jim.7000311