Effect of nickel deprivation on methanol degradation in a methanogenic granular sludge bioreactor

M.H. Zandvoort, M.B. Osuna, R. Geerts, G. Lettinga, P.N.L. Lens

Research output: Contribution to journalArticleAcademicpeer-review

30 Citations (Scopus)

Abstract

The effect of omitting nickel from the influent on methanol conversion in an Upflow Anaerobic Sludge Bed (UASB) reactor was investigated. The UASB reactor (30°C, pH 7) was operated for 261 days at a 12-h hydraulic retention time (HRT) and at organic loading rates (OLRs) ranging from 2.6 to 7.8 g COD l reactorm1 daym1. The nickel content of the sludge decreased by 66 during the 261-day reactor run because of washout and doubling of the sludge bed volume. Nickel deprivation initially had a strong impact on the methanogenic activity of the sludge with methanol; e.g., after 89 days of operation, this activity was doubled by adding 2 wM nickel. Upon prolonged UASB reactor operation, methanol and VFA effluent concentrations decreased whereas the sludge lost its response to nickel addition in activity tests. This suggests that a less nickel-dependent methanol-converting sludge had developed in the UASB reactor.
Original languageEnglish
Pages (from-to)268-274
JournalJournal of Industrial Microbiology and Biotechnology
Volume29
DOIs
Publication statusPublished - 2002

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